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Các phương pháp multiplex qPCR để phân biệt các loài nấm mycorrhiza arbuscular riêng lẻ từ rễ và đất
Tóm tắt
Hiện nay, các phép đo sự định cư của nấm mycorrhiza arbuscular (AMF) yêu cầu nhuộm màu và quan sát dưới kính hiển vi, và việc xác định loài nấm ở cấp độ loài thông qua những quan sát này là không khả thi. Ở đây, chúng tôi trình bày các phương pháp multiplex PCR thời gian thực mới nhắm vào các gene glomalin của 11 loài AMF khác nhau thường gặp trong đất nông nghiệp ôn đới, cho phép phát hiện độc lập và đo lường sự phong phú của những nấm này bằng cách sử dụng chiết xuất DNA từ đất và/hoặc mô rễ. Sự có mặt của những công cụ này sẽ không chỉ tăng khả năng thông qua trong việc xác định mức độ định cư của rễ mà còn có thể cung cấp các mức độ định cư ở loài cụ thể từ một mẫu duy nhất. Điều này sẽ giúp xác định những loài AMF nào, hoặc sự kết hợp của các loài khác nhau, mang lại lợi ích cao nhất cho cây trồng, và sẽ hỗ trợ trong việc phát triển AMF để sử dụng như phân bón sinh học.
Từ khóa
#nấm mycorrhiza arbuscular #AMF #multiplex qPCR #glomalin #định cư của rễ #phân bón sinh họcTài liệu tham khảo
Bodenhausen N, Deslandes-Hérold G, Waelchli J, Held A, van der Heijden MG, Schlaeppi K (2021) Relative qPCR to quantify colonization of plant roots by arbuscular mycorrhizal fungi. Mycorrhiza 31(2):137–148
Bona E, Cantamessa S, Massa N, Manassero P, Marsano F, Copetta A, Lingua G, D’Agostino G, Gamalero E, Berta G (2017) Arbuscular mycorrhizal fungi and plant growth-promoting pseudomonads improve yield, quality and nutritional value of tomato: a field study. Mycorrhiza 27:1–11
Comas LH, Callahan HS, Midford PE (2014) Patterns in root traits of woody species hosting arbuscular and ectomycorrhizas: implications for the evolution of belowground strategies. Ecol Evol 4(15):2979–2990
Douds DD, Nagahashi G, Pfeffer PE, Reider C, Kayser WM (2006) On-farm production of AM fungus inoculum in mixtures of compost and vermiculite. Biores Technol 97(6):809–818. https://doi.org/10.1016/j.biortech.2005.04.015
Douds D Jr, Carr E, Shenk J, Ganser S (2017) Positive yield response of eggplant (Solanum melongena L.) to inoculation with AM fungi produced on-farm. Sci Hortic 224:48–52
Douds D Jr, Galvez L, Janke R, Wagoner P (1995) Effect of tillage and farming system upon populations and distribution of vesicular-arbuscular mycorrhizal fungi. Agr Ecosyst Environ 52(2–3):111–118
Douds D Jr, Lee J, Shenk J, Ganser S (2015) Inoculation of sweet potatoes with AM fungi produced on-farm increases yield in high P soil. J Appl Hortic 17(3):171–175
Douds D Jr, Reider C (2003) Inoculation with mycorrhizal fungi increases the yield of green peppers in a high P soil. Biol Agric Hortic 21(1):91–102
Douds DD Jr, Nagahashi G, Reider C, Hepperly PR (2007) Inoculation with arbuscular mycorrhizal fungi increases the yield of potatoes in a high P soil. Biol Agric Hortic 25(1):67–78
Douds DD Jr, Nagahashi G, Shenk JE, Demchak K (2008) Inoculation of strawberries with AM fungi produced on-farm increased yield. Biol Agric Hortic 26(3):209–219
Evans D, Miller M (1990) The role of the external mycelial network in the effect of soil disturbance upon vesicular—arbuscular mycorrhizal colonization of maize. New Phytol 114(1):65–71
Galvez L, Douds D, Drinkwater L, Wagoner P (2001) Effect of tillage and farming system upon VAM fungus populations and mycorrhizas and nutrient uptake of maize. Plant Soil 228(2):299–308
Gamper HA, Young JPW, Jones DL, Hodge A (2008) Real-time PCR and microscopy: are the two methods measuring the same unit of arbuscular mycorrhizal fungal abundance? Fungal Genet Biol 45(5):581–596
Giovannetti M, Mosse B (1980) An evaluation of techniques for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol 489–500
Jansa J, Mozafar A, Kuhn G, Anken T, Ruh R, Sanders I, Frossard E (2003) Soil tillage affects the community structure of mycorrhizal fungi in maize roots. Ecol Appl 13(4):1164–1176
Jansa J, Smith FA, Smith SE (2008) Are there benefits of simultaneous root colonization by different arbuscular mycorrhizal fungi? New Phytol 177(3):779–789
Jansa J, Wiemken A, Frossard E (2006) The effects of agricultural practices on arbuscular mycorrhizal fungi. Geological Society, London, Special Publications 266(1):89–115
Jasper D, Abbott L, Robson A (1989) Soil disturbance reduces the infectivity of external hyphae of vesicular—arbuscular mycorrhizal fungi. New Phytol 112(1):93–99
Kabir Z, O'halloran I, Widden P, Hamel C (1998) Vertical distribution of arbuscular mycorrhizal fungi under corn (Zea mays L) in no-till and conventional tillage systems. Mycorrhiza 8(1):53–55
Knegt B, Jansa J, Franken O, Engelmoer DJ, Werner GD, Bücking H, Kiers ET (2016) Host plant quality mediates competition between arbuscular mycorrhizal fungi. Fungal Ecol 20:233–240
Kokkoris V, Stefani F, Dalpé Y, Dettman J, Corradi N (2020) Nuclear dynamics in the arbuscular mycorrhizal fungi. Trends Plant Sci 25(8):765–778
Krüger M, Stockinger H, Krüger C, Schüßler A (2009) DNA-based species level detection of Glomeromycota: one PCR primer set for all arbuscular mycorrhizal fungi. New Phytol 183(1):212–223
Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 35(6):1547
Magurno F, Malicka M, Posta K, Wozniak G, Lumini E, Piotrowska-Seget Z (2019) Glomalin gene as molecular marker for functional diversity of arbuscular mycorrhizal fungi in soil. Biol Fertil Soils 55(4):411–417. https://doi.org/10.1007/s00374-019-01354-x
McGonigle T, Miller M, Evans D, Fairchild G, Swan J (1990) A new method which gives an objective measure of colonization of roots by vesicular—arbuscular mycorrhizal fungi. New Phytol 115(3):495–501
Morin E, Miyauchi S, San Clemente H, Chen EC, Pelin A, de la Providencia I, Ndikumana S, Beaudet D, Hainaut M, Drula E (2019) Comparative genomics of Rhizophagus irregularis, R. cerebriforme, R. diaphanus and Gigaspora rosea highlights specific genetic features in Glomeromycotina. New Phytol 222(3):1584–1598
Morton J, Bentivenga S, Wheeler W (1993) Germ plasm in the International Collection of Arbuscular and Vesicular-Arbuscular Mycorrhizal Fungi (INVAM) and procedures for culture development, documentation and storage. Mycotaxon 48(1):491–528
Rosier CL, Piotrowski JS, Hoye AT, Rillig MC (2008) Intraradical protein and glomalin as a tool for quantifying arbuscular mycorrhizal root colonization. Pedobiologia 52(1):41–50
Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4(4):406–425
Scholdberg TA, Norden TD, Nelson DD, Jenkins GR (2009) Evaluating precision and accuracy when quantifying different endogenous control reference genes in maize using real-time PCR. J Agric Food Chem 57(7):2903–2911
Schwarzott D, Schüßler A (2001) A simple and reliable method for SSU rRNA gene DNA extraction, amplification, and cloning from single AM fungal spores. Mycorrhiza 10(4):203–207
Six J, Bossuyt H, Degryze S, Denef K (2004) A history of research on the link between (micro) aggregates, soil biota, and soil organic matter dynamics. Soil and Tillage Research 79(1):7–31
Six J, Elliott E, Paustian K (2000) Soil macroaggregate turnover and microaggregate formation: a mechanism for C sequestration under no-tillage agriculture. Soil Biol Biochem 32(14):2099–2103
Smith SE, Read D (2008) Mycorrhizal Symbiosis. In: Smith SE, Read D (eds) Mycorrhizal Symbiosis (Third Edition). Academic Press, London, pp vii-ix. https://doi.org/10.1016/B978-012370526-6.50001-5
Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc Natl Acad Sci 101(30):11030–11035
Thonar C, Erb A, Jansa J (2012) Real-time PCR to quantify composition of arbuscular mycorrhizal fungal communities—marker design, verification, calibration and field validation. Mol Ecol Resour 12(2):219–232
Tisserant E, Malbreil M, Kuo A, Kohler A, Symeonidi A, Balestrini R, Charron P, Duensing N, Frei Dit Frey N, Gianinazzi-Pearson V, Gilbert LB, Handa Y, Herr JR, Hijri M, Koul R, Kawaguchi M, Krajinski F, Lammers PJ, Masclaux FG, Murat C, Morin E, Ndikumana S, Pagni M, Petitpierre D, Requena N, Rosikiewicz P, Riley R, Saito K, San Clemente H, Shapiro H, Van Tuinen D, Becard G, Bonfante P, Paszkowski U, Shachar-Hill YY, Tuskan GA, Young JPW, Sanders IR, Henrissat B, Rensing SA, Grigoriev IV, Corradi N, Roux C, Martin F (2013) Genome of an arbuscular mycorrhizal fungus provides insight into the oldest plant symbiosis. Proc Natl Acad Sci 110(50):20117–20122. https://doi.org/10.1073/pnas.1313452110
Venice F, Ghignone S, Salvioli di Fossalunga A, Amselem J, Novero M, Xianan X, Sędzielewska Toro K, Morin E, Lipzen A, Grigoriev IV (2020) At the nexus of three kingdoms: the genome of the mycorrhizal fungus Gigaspora margarita provides insights into plant, endobacterial and fungal interactions. Environ Microbiol 22(1):122–141
Wright SF, Upadhyaya A (1996) Extraction of an abundant and unusual protein from soil and comparison with hyphal protein of arbuscular mycorrhizal fungi. Soil Sci 161(9):575–586
