Các phương pháp multiplex qPCR để phân biệt các loài nấm mycorrhiza arbuscular riêng lẻ từ rễ và đất

Mycorrhiza - Tập 32 - Trang 155-164 - 2022
Wade P. Heller1, Joseph E. Carrara1
1USDA Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, USA

Tóm tắt

Hiện nay, các phép đo sự định cư của nấm mycorrhiza arbuscular (AMF) yêu cầu nhuộm màu và quan sát dưới kính hiển vi, và việc xác định loài nấm ở cấp độ loài thông qua những quan sát này là không khả thi. Ở đây, chúng tôi trình bày các phương pháp multiplex PCR thời gian thực mới nhắm vào các gene glomalin của 11 loài AMF khác nhau thường gặp trong đất nông nghiệp ôn đới, cho phép phát hiện độc lập và đo lường sự phong phú của những nấm này bằng cách sử dụng chiết xuất DNA từ đất và/hoặc mô rễ. Sự có mặt của những công cụ này sẽ không chỉ tăng khả năng thông qua trong việc xác định mức độ định cư của rễ mà còn có thể cung cấp các mức độ định cư ở loài cụ thể từ một mẫu duy nhất. Điều này sẽ giúp xác định những loài AMF nào, hoặc sự kết hợp của các loài khác nhau, mang lại lợi ích cao nhất cho cây trồng, và sẽ hỗ trợ trong việc phát triển AMF để sử dụng như phân bón sinh học.

Từ khóa

#nấm mycorrhiza arbuscular #AMF #multiplex qPCR #glomalin #định cư của rễ #phân bón sinh học

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