Multiple functions for NGF receptor in developing, aging and injured rat teeth are suggested by epithelial, mesenchymal and neural immunoreactivity

Development (Cambridge) - Tập 109 Số 2 - Trang 461-471 - 1990
Margaret R. Byers1, Gina C. Schatteman1, Mark Bothwell1
1University of Washington Departments of Anesthesiology, Biological Structure, Physiology and Biophysics , , RN-10 Seattle, WA 98195 USA

Tóm tắt

ABSTRACT We have used immunocytochemistry to analyse expression of nerve growth factor receptor (NGFR) in developing, aging and injured molar teeth of rats. The patterns of NGFR immunoreactivity (IR) in developing epithelia and mesenchyme matched the location of NGFR mRNA assayed by in situ hybridization with a complementary S35-labeled RNA probe. The following categories of NGFR expression were found. (1) There was NGFR-IR in the dental lamina epithelium and in adjacent mesenchyme during early stages of third molar formation. (2) NGFR-IR nerve fibers were posterior and close to the bud epithelium. (3) During crown morphogenesis NGFR expression was prominent in internal enamel epithelium and preodontoblasts; it faded as preameloblasts elongated and as odontoblasts began to make predentin matrix; and it was weak or absent from outer enamel epithelium, the cervical loop, and differentiated ameloblasts and odontoblasts. (4) When NGFR-IR nerve fibers entered the molars late in the bell stage, they innervated the most mature peripheral pulp and dentin in an asymmetric pattern which correlated more with asymmetric enamel synthesis than with mesenchymal NGFR-IR distribution. (5) The mesenchymal pulp cells continued to have intense NGFR expression in adult teeth, especially near coronal tubular dentin. (6) The pulpal NGFR-IR decreased in very old rats or subjacent to reparative dentin (naturally occurring or experimentally induced). (7) During root formation, the preodontoblasts had NGFR-IR but most root mesenchymal cells and Hertwig’s epithelial root sheath did not. This work suggests that there are important epithelial and mesenchymal targets of NGF regulation during molar morphogenesis that differ for crown and root development and that do not correlate with neural development. The continuing expression of NGFR-IR by pulpal mesenchymal cells in adult rats was most intense near coronal odontoblasts making tubular dentin; and it was lost during aging, or subjacent to sites of dentin injury that caused a phenotypic change in the odontoblast layer.

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Tài liệu tham khảo

Angerer, 1987, In situ hybridization with RNA probes: an annotated recipe. In In Situ Hybridization Applications to Neurobiology’, 42

Byers, 1984, Dental sensory receptors, Int. Rev. Neurobiol., 25, 39, 10.1016/S0074-7742(08)60677-7

Byers, 1990, Segregation of NGF-receptor in sensory receptors, nerves and local cells of teeth and periodontium demonstrated by EM-immunocytochemistry, J. Neurocytol. (In Press), 10.1007/BF01188044

Byers, 1988, Interactions of nerves and pulp cells in normal and injured teeth analysed by LM and EM immunocytochemistry for NGF-receptor, Neursci. Abstr., 14, 1167

Casasco, 1989, Immunocytochemical detection of proliferating cells in the rat molar germ by monoclonal antibodies against 5-bromo-2’-deoxyuridine, Arch, oral Biol., 34, 65, 10.1016/0003-9969(89)90047-2

Chandler, 1984, A monoclonal antibody modulates the interaction of nerve growth factor with PC 12 cells, J. biol. Chem., 259, 6882, 10.1016/S0021-9258(17)39810-1

Chesa, 1988, Immunohistochemical analysis of nerve growth factor receptor expression in normal and malignant human tissues, J. Histochem. Cytochem., 36, 383, 10.1177/36.4.2831267

Davies, 1987, Timing and site of nerve growth factor synthesis in developing skin in relation to innervation and expression of the receptor, Nature, 326, 353, 10.1038/326353a0

Davies, 1984, Relation of target encounter and neuronal death to nerve growth factor responsiveness in the developing mouse trigeminal ganglion, J. comp. Neurol., 223, 124, 10.1002/cne.902230110

Johnson, 1988, Expression and possible function of nerve growth factor receptors on Schwann cells, Trends in Neurosci., 7, 299, 10.1016/0166-2236(88)90090-2

Jontell, 1988, Dendritic cells and macrophages expressing class II antigens in the normal rat incisor pulp, J. dent. Res., 67, 1263, 10.1177/00220345880670100301

Koch, 1967, In vitro differentiation of tooth rudiments of embryonic mice. I. Transfilter interaction of embryonic incisor tissues, J. exp. Zool., 165, 155, 10.1002/jez.1401650202

Kollar, 1983, Epithelial-mesenchymal interactions in the mammalian integument: Tooth development as a model for instructive induction, Epithelial-Mesenchymal Interactions in Development, 27

Kollar, 1980, Tooth induction in chick epithelium: Expression of quiescent genes for enamel synthesis, Science, 207, 993, 10.1126/science.7352302

Kollar, 1979, Tooth morphogenesis: The role of the innervation during induction and pattern formation, J. Biol. Buccale, 7, 49

Levi-Montalcini, 1987, The nerve growth factor: 35 years later, Science 231. 1154-1162, 10.1126/science.3306916

Lumsden, 1988, Spatial organization of the epithelium and the role of neural crest cells in the initiation of the mammalian tooth germ, Development 103 (Supplement) 155-169, 10.1242/dev.103.Supplement.155

Lumsden, 1986, An experimental study of timing and topography of early tooth development in the mouse embryo with an analysis of the role of innervation, Arch, oral Biol., 31, 301, 10.1016/0003-9969(86)90044-0

Mina, 1987, The induction of odontogenesis in non-dental mesenchyme combined with early murine mandibular arch epithelium, Arch, oral Biol., 32, 123, 10.1016/0003-9969(87)90055-0

Partanen, 1984, Transferrin is required for early tooth morphogenesis, Differentiation, 27, 59, 10.1111/j.1432-0436.1984.tb01408.x

Partanen, 1987, Localization and quantification of 125I-epidermal growth factor binding in mouse embryonic tooth and other embryonic tissues at different developmental stages, Devi Biol., 120, 186, 10.1016/0012-1606(87)90117-5

Raivich, 1987, The spatial and temporal pattern of /3-NGF receptor expression in the developing chick embryo, EMBO J., 4, 637, 10.1002/j.1460-2075.1985.tb03677.x

Repressa, 1989, Nerve growth factor and serum differentially regulate development of the embryonic otic vesicle and cochleovestibular ganglion in vitro, Devi Biol., 134, 21, 10.1016/0012-1606(89)90074-2

Ross, 1984, Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies, Proc. natn. Acad. Sci. U.S.A., 81, 6681, 10.1073/pnas.81.21.6681

Ruch, 1985, Odontoblast differentiation and the formation of the odontoblast layer, J. dent. Res., 64, 489, 10.1177/002203458506400402

Silverman, 1987, An interpretation of dental innvervation based upon the pattern of calcitonin generelated peptide (CGRP) immunoreactive thin sensory axons, Somatos. Res., 5, 157, 10.3109/07367228709144624

Slavkin, 1988, Gene regulation in the development of oral tissues, J. dent. Res., 67, 1142, 10.1177/00220345880670090101

Slavkin, 1988, Molecular determinants of cranial neural crest-derived odontogenic ectomesenchyme during dentinogenesis, Am. J. med. Cen. Suppl, 4, 7

Taylor, 1988, Sprouting of CGRP nerve fibers in response to dentin injury, Brain Res, 461, 371, 10.1016/0006-8993(88)90270-3

Taylor, 1990, Response of CGRP-immunoreactive nerve fibers to pulpitis and healing in rat molars, Arch. Oral Biol., in press

Thesleff, 1981, Tissue interactions in tooth development, Differentiation, 18, 75, 10.1111/j.1432-0436.1981.tb01107.x

Thomas, 1985, The dentin-predentin complex and its permeability: an anatomical overview, J. dent. Res., 64, 607, 10.1177/002203458506400418

Thompson, 1989, A monoclonal antibody against nerve growth factor receptor: Immunohistochemical analysis of normal and neoplastic human tissue, Am. J. Clin. Pathol., 92, 415, 10.1093/ajcp/92.4.415

Vainio, 1989, Syndecan and tenascin expression is induced by epithelial-mesenchymal interactions in embryonic tooth mesenchyme, J. Cell Biol., 108, 1945, 10.1083/jcb.108.5.1945

Yan, 1988, An immunohistochemical study of the nerve growth factor receptor in developing rats, J. Neurosci., 8, 3481, 10.1523/JNEUROSCI.08-09-03481.1988

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Development (Cambridge)

Tập 109 Số 2

461-471

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