Multidrug‐resistance (MDR) phenotype of human osteosarcoma cells evaluated by quantitative morphological and electron microscopy analyses

Biology of the Cell - Tập 84 - Trang 195-204 - 1995
Nicoletta Zini1, Katia Scotlandi2, Nicola Baldini1, Giuseppe Nini2, Patrixia Sabatelli1, Nadir M Maraldi1,3,4
1Istituto di Citomorfologia Normale e Patologica, CNR, Chieti-Bologna, Sezione di Bologna, Italy
2Laboratorio di Ricerca Oncologica, IOR, via di Barbiano 1/10, 40136 Bologna, Italy
3Laboratorio di Biologia Cellulare e Microscopia Elettronica, IOR, via di Barbiano 1/10, 40136 Bologna, Italy
4Unità Complessa di Scienze Anatomiche Umane e Fisiopatologia dell'Apparato Locomotore, University of Bologna, via Irnerio 48, 40126 Bologna, Italy

Tóm tắt

Summary— Multidrug‐resistant (MDR) variants of a human osteosarcoma cell line (U‐2 OS) have been recently obtained by continuous exposure to doxorubicin (DX). The growth and phenotypic characteristics of these cell lines have been demonstrated to be related to the level of expression of P‐glycoprotein. In this work, the morphological changes associated with MDR have been evaluated by quantitative image analysis and transmission electron microscopy. Resistant cells present morphological changes with respect to sensitive cells at both cytoplasmic and nuclear level. Some of these changes appear to be related to the degree of resistance but not to the direct presence of DX, since deprived cells maintain some modified characters, while others are partly lost. These findings suggest that DX exposure affects cell metabolism causing progressive changes of the cell morphotype.

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Biology of the Cell

Tập 84

195-204

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