Multidrug Resistant Listeria Species from Milk and Milk Products

Nitasha Sambyal1, M. Rashid1, S. K. Kotwal1, Majueeb U. Rehman1
1Division of Veterinary Public Health and Epidemiology, Sher-e Kashmir University of Agricultural Sciences and Technology, Jammu, India

Tóm tắt

A total of 295 samples from milk (110), kalaari (110), ice-cream (75) were analysed to reveal the prevalence of Listeria species in and around Jammu. The overall prevalence of Listeria species was 8 (2.71 %). Listeria monocytogenes was isolated from 7 samples (2.37 %) which included 3 (2.72 %) in milk, 4 (3.63 %) in kalaari and one isolate of Listeria ivanovii (4.54 %) from ice-cream. The prevalence was higher in winter months (p < 0.05). All isolates of L. monocytogenes (n = 7) and L. ivanovii (n = 1) were hemolytic and Christie, Atkins, Munch-Peterson tests were positive while 5 L. monocytogenes, one L. ivanovii were phosphatidylinositol specific phospholipases C positive. All Listeria isolates were sensitive to Amoxicillin + Sulbactam Ampicillin, Erythromycin and Enrofloxacin, Levofloxacin, Gentamycin and multidrug resistant to Amikacin, Cephalexin, Cefixime, Cefuroxime and Oleandomicin.

Tài liệu tham khảo

Low JC, Donachie W (1997) A review of Listeria monocytogenes and listeriosis. Vet J 153:9–29 Wing EJ, Gregory SH (2000) An updated model of cell mediated immunity- listeriosis clinical and research aspects. Allergy Asthma Proc 21:209–214 Mead P, Slutsker L, Dietz V, McCaig L, Bresee J, Shapiro C, Griffin P, Tauxe R (1999) Food-related illness and death in the United States. Emerg Infect Dis 5(5):607–625 Varnam AH (1991) Foodborne pathogens. Wolfe Publishing Ltd., London, pp 327–353 Bockserman R (2000). Listeria monocytogenes: recognized threat to food safety. Food Qual Mag. www.Fqmagazine.com Dawson SJ (2006) Listeria outbreak associated with sandwich consumption from a hospital retail shop, United Kingdom. Eur Surveill 11:89 Thunberg RL, Tran TT, Bennet RW, Mathews RN, Belay N (2002) Microbial evaluation of fresh produce obtained at retail markets. J Food Prod 65:677–682 Rawool DB, Malik SVS, Shakuntala I, Sahare AM, Barbuddhe SB (2007) Detection of multiple virulence-associated genes in Listeria monocytogenes isolated from bovine mastitis cases. Int J Food Microbiol 113(2):201–207 Notermans SHW, Dufrenne J, Leimeister-Wachter M, Domann E, Chakraborty T (1991) Phosphatidylinositol-specific phospholipase C activity as a marker to distinguish between pathogenic and non-pathogenic Listeria species. Appl Environ Microbiol 57:2666–2670 Notermans S, Dufrenne J, Chakraborty T, Steinmeyer S, Terplant G (1991) The chick embryo test agrees with the mouse bio-assay for assessment of the pathogenicity of Listeria species. Lett Appl Microbiol 13:161–164 Menudier A, Bosiraud C, Nicolas JA (1991) Virulence of Listeria monocytogenes serovars and Listeria spp. in experimental infection of mice. J Food Prod 54:917–921 Skovgaard N, Morgen CA (1988) Detection of Listeria species in faeces from animals, in feeds and in raw foods of animal origin. Int J Food Microbiol 6:229–242 Bhat SA, Willayat MM, Roy SS, Qureshi S, Sharief J (2011) Isolation and molecular characterisation of Listeria monocytogenes from Mutton and Paneer. J Vet Public Health 9(1):19–24 McClain D, Lee WH (1988) Development of USDA-FSIS method for isolation of Listeria monocytogenes from raw meat and poultry. J Assoc Off Anal Chem 71:660–664 Curtis GDW, Lee WH (1995) Culture media and methods for the isolation of Listeria monocytogenes strains. Int J Food Microbiol 26:1–13 Seeliger HPR, Jones D (1986) Genus Listeria pirie, 1940, 383AL. Bergey’s manual of systematic bacteriology, vol 2. Williams and Wilkins, Baltimore, pp 1235–1245 Holt JG, Krieg NR, Sneath PHA, Staley JT, Williams ST (1994) Bergey’s manual of determinative bacteriology, 9th edn. Williams and Wilkins, Baltimore, pp 566–570 Cruickshank R, Duguid JP, Marimon BP, Swain BP (1975) Medical microbiology, 12th edn. Churchill Livingstone, Edinburgh Barrow GI, Feltham RKA (1993) Cowan and steel’s manual for the identification of medical bacteria, 3rd edn. Cambridge University Press, Cambridge, pp 140–143 BIS (1994). Microbiology- general guidance for the detection of Listeria monocytogenes. Committee Draft, CD, 11290 Bauer AW, Kirby WMM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disc method. Am J Clin Pathol 45(4):493–496 Yadav MM, Roy A, Bhanderi B, Joshi C (2010) Phenol-genotypic characterization of L. monocytogenes from bovine clinical mastitis. Buffalo. Bulletin 29(1):28–39 Kalorey DR, Warke SR, Kurkure NV, Rawool DB, Barbuddhe SB (2008) Listeria spp. in bovine raw milk: a large survey of central India. Food Control 19:109–112 Shakuntala I, Malik SVS, Barbuddhe SB, Rawool DB (2006) Isolation of Listeria monocytogenes from buffaloes with reproductive disorders and its confirmation by polymerase chain reaction. Vet Microbiol 117:229–234 Kaur S, Malik SVS, Vaidya VM, Barbuddhe SB (2007) Listeria monocytogenes in spontaneous abortion in humans and its detection by multiplex PCR. J Appl Microbiol 103:1889–1896 MacGowan AP (1990) Listeriosis the therapeutic option. J Antimicrobiol Chemother 26:721–722 Riviera L, Dubini F, Belloti MG (1993) Listeria monocytogenes infections: the organism, its pathogenicity and antimicrobial drugs susceptibility. Microbiologica 16:198–204