MultiPrimer

Chungnan Lee1, Jain-Shing Wu1, Yow-Ling Shiue2, Hong-Long Liang1
1Department of Computer Science and Engineering, National Sun Yat-sen University, Kaohsiung, Taiwan
2Institute of Biomedical Sciences, National Sun Yat-sen University, Kaohsiung, Taiwan

Tóm tắt

This study presents a novel application, MultiPrimer, to assist users to design multiplex PCR primers. MultiPrimer adopts an algorithm based on a genetic algorithm and can efficiently solve the multi-objective optimisation problem. A match pattern model is proposed to speed up examination of the specificity constraint. MultiPrimer is able to find a set of primer pairs simultaneously for a set of gene family sequences and multiple targets for a single cDNA sequence. A public version of the MultiPrimer software is available from http://edith.cse.nsysu.edu.tw/new/software/MultiPrimer.htm .

Từ khóa


Tài liệu tham khảo

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