Mucosubstance histochemistry of pleomorphic adenoma of parotid and submandibular salivary glands of man: light and electron microscopy
Tóm tắt
Lumina and adluminal cells in human salivary gland pleomorphic adenomas were found to contain neutral, carboxylated, and occasionally sulphated glycoproteins. A variable component of luminal contents and secretory granules did not appear to contain glycoprotein and possibly consisted of protein. Glycosaminoglycans, which appeared to be hyaluronic acid and chondroitin sulphate, were demonstrated rarely in lumina, often between epithelial cells, and forming the matrix of myxoid tissue and, together with collagen, chondroid tissue. No differences were seen between tumours from parotid glands and those from submandibular glands. Glycoproteins demonstrated in the epithelium are similar to those of intercalary ducts of parotid and submandibular glands, and may represent a primitive form of salivary secretion. Glycosaminoglycans secreted intercellularly by epithelial cells cause their increasing separation to form myxoid or chondroid tissue. This stromalization extends to lumina to produce a loss of epithelium. Pleomorphic adenoma appears to be a manifest example of variable derepression of the genotype.
Tài liệu tham khảo
Auger, D. W. (1985) Ultrastructural and histochemical studies on pleomorphic adenoma of parotid and submandibular salivary glands of man. PhD Thesis, University of London.
Azzopardi, J. G. &Smith, O. D. (1959) Salivary gland tumours and their mucins.J. Pathol. 77, 131–40.
Azzopardi, J. G. &Zayid, I. (1972) Elastic tissue in tumours of salivary glands.J. Pathol. 107, 149–56.
Behnke, O. &Zelander, T. (1970) Preservation of intercellular substances by the cationic dye Alcian blue in preparative procedures for electron microscopy.J. Ultrastruct. Res. 31, 424–38.
Brandes, G. &Reale, E. (1990) The reaction of Acridine Orange with proteoglycans in the articular cartilage of the rat.Histochem. J. 22, 106–12.
Caselitz, J. (1987) Das pleomorphe Adenom der Speicheldrüsen. Histogenese, zelluläre Differenzierung, Tumormarker.Veroff. Pathol.126.
Chisholm, D. M., Waterhouse, J. P., Kraucunas, E. &Sciubba, J. J. (1974) A quantitative ultrastructural study of the pleomorphic adenoma (mixed tumor) of human minor salivary glands.Cancer 34, 1631–41.
Cutler, L. S., Christian, C. P. &Rendell, J. K. (1987) Glycosaminoglycan synthesis by adult rat submandibular salivary-gland secretory units.Arch. Oral Biol. 32, 413–9.
Dardick, I., Van Nostrand, A. W. P., Jeans, M. T. D., Rippstein, P. &Edwards, V. (1983a) Pleomorphic adenoma, I: ultrastructural organization of ‘epithelial’ regions.Hum. Pathol. 14, 780–97.
Dardick, I., Van Nostrand, A. W. P., Jeans, M. T. D., Rippstein, P. &Edwards, V. (1983b) Pleomorphic adenoma, II: ultrastructural organization of ‘stromal’ regions.Hum. Pathol. 14, 798–809.
David, R. &Buchner, A. (1982) Tannic acid-glutaraldehyde fixative and pleomorphic adenomas of the salivary gland: an ultrastructural studyJ. Oral Pathol. 11, 26–38.
Donath, K., Dietrich, H. &Seifert, G. (1978) Entwicklung und ultrastrukturelle Cytodifferenzierung der Parotis des Meschen.Virchows Arch. (A) 378, 297–314.
Erlandson, R. A., Cordon-Cardo, C. &Higgins, P. J. (1984) Histogenesis of benign pleomorphic adenoma (mixed tumor) of the major salivary glands. An ultrastructural and immunohistochemical study.Am J. Surg. Pathol. 8, 803–20.
Gibson, M. H. L. (1983) The prenatal human submandibular gland: a histological, histochemical and ultrastructural study.Anat. Anz. 153, 91–105.
Hamperl, H. (1970) The myothelia (myoepithelial cells). Normal state; regressive changes; hyperplasia; tumors.Curr. Top. Pathol. 53, 161–220.
Harrison, J. D. (1973) Odontogenic myxoma: ultrastructural and histochemical studies.J. Clin. Pathol. 26, 570–82.
Harrison, J. D. (1974) Cystic adenoma of a minor salivary gland: a histochemical study.J. Pathol. 114, 29–38.
Harrison, J. D. &Auger, D. W. (1982) Ultrastructural cytochemistry of phosphatases in the ductal component of pleomorphic adenoma of human parotid and submandibular salivary glands.Histochem. J. 14, 703–11.
Harrison, J. D. &Auger, D. W. (1989a) Ultrastructural morphology of secretory granules in pleomorphic adenoma of human parotid and submandibular salivary glands.Arch. Oral Biol. 34, 759–61.
Harrison, J. D. &Auger, D. W. (1989b) Ultrastructural observations on luminal structures of pleomorphic adenoma of parotid and submandibular salivary glands of man.Virchows Arch. (A) 415, 559–63.
Harrison, J. D., Auger, D. W., Badir, M. S. &Paterson, K. L. (1987a) Ultrastructural morphology of secretory granules of submandibular and parotid salivary glands of man.Arch. Oral Biol 32, 229–34.
Harrison, J. D., Auger, D. W., Paterson, K. L. &Rowley, P. S. A. (1987b) Mucin histochemistry of submandibular and parotid salivary glands of man: light and electron microscopyHistochem. J. 19, 555–64.
Harrison, J. D. &Rose, P. E. (1985) Myxoid meningioma: histochemistry and electron microscopy.Acta Neuropathol. (Berl.) 68, 80–2.
Hascall, V. C. &Hascall, G. K. (1981) Proteoglycans. InCell Biology of Extracellular Matrix (edited byHay, E. D.) pp. 39–63. New York: Plenum.
Karnovsky, M. J. (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy.J. Cell Biol. 27, 137A-8A.
Lam, R. M. Y. (1985) An electron microscopic histochemical study of the histogenesis of major salivary gland pleomorphic adenoma.Ultrastruct. Pathol. 8, 207–23.
Line, S. R. P., Torloni, H. &Junqueira, L. C. U. (1989) Diversity of collagen expression in the pleomorphic adenoma of the parotid gland.Virchows Arch. (A) 414, 477–83.
Palmer, R. M., Lucas, R. B., Knight, J. &Gusterson, E. (1985a) Immunocytochemical identification of cell types in pleomorphic adenoma, with particular reference to myoepithelial cells.J. Pathol. 146, 213–20.
Palmer, R. M., Lucas, R. B. &Langdon, J. D. (1985b) Ultrastructural analysis of salivary gland pleomorphic adenoma, with particular reference to myoepithelial cells.Histopathology 9, 1061–76.
Quintarelli, G. &Dellovo, M. C. (1966) Age changes in the localization and distribution of glycosaminoglycans in human hyaline cartilage.Histochemie 7, 141–67.
Quintarelli, G. &Robinson, L. (1967) The glycosaminoglycans of salivary gland tumors. A histochemical characterization and a critical evaluation.Am. J. Pathol. 51, 19–37.
Reale, E., Luciano, L. &Spitznas, M. (1986) Histochemical demonstration of hyaluronic acid molecules by Alcian Blue.Histochem. J. 18, 306–16.
Saku, T., Cheng, J., Okabe, H. &Koyama, Z. (1990) Immunolocalization of basement membrane molecules in the stroma of salivary gland pleomorphic adenoma.J. Oral Pathol. Med 19, 208–14.
Sannes, P. L., Katsuyama, T. &Spicer, S. S. (1978) Tannic acid-metal salt sequences for light and electron microscopic localization of complex carbohydrates.J. Histochem. Cytochem. 26, 55–61.
Scott, J. E. &Dorling, J. (1965) Differential staining of acid glycosaminoglycans (mucopolysaccharides) by Alcian Blue in salt solutions.Histochemie 5, 221–33.
Seipert, G., Miehlke, A., Haubrich, J. &Chilla, R. (1986) Pleomorphic adenoma. InDiseases of the Salivary Glands. Pathology, Diagnosis, Treatment, Facial Nerve Surgery (Translated byStell, P. M.) pp. 182–94. Stuttgart New York: Thieme.
Shimada, Y. &Oota, K. (1954) Histochemical studies of the so-called mixed tumors of salivary gland type and related tissues.Gann 45, 233–8.
Spicer, S. S., Horn, R. G. &Leppi, T. J. (1967) Histochemistry of connective tissue mucopolysaccharides. InThe Connective Tissue (edited byWagner, B. M. &Smith, D. E.) pp. 251–303. Baltimore: Williams & Wilkins.
Stoward, P. J. (1967) The histochemical properties of some periodate-reactive mucosubstances of the pregnant Syrian hamster before and after methylation with methanolic thionyl chloride.J. R. Microsc. Soc. 87, 77–103.
Takeuchi, J., Sobue, M., Yoshida, M., Esaki, T. &Katoh, Y. (1975) Pleomorphic adenoma of the salivary gland with special reference to histochemical and electron microscopic studies and biochemical analysis of glycosaminoglycans in vivo and in vitro.Cancer 36, 1771–84.
Takeuchi, J., Sobue, M., Yoshida, M. &Sato, E. (1978) Glycosaminoglycan-synthetic activity of pleomorphic adenoma, adenoid cystic carcinoma and nonneoplastic tubuloacinar cells of the salivary gland.Cancer 42, 202–8.
Tandler, B. (1971) Ultrastructure of adenoid cystic carcinoma of salivary gland origin.Lab. Invest 24, 504–12.
Thackray, A. C. &Lucas, R. B. (1974) Pleomorphic adenoma (mixed tumor). InTumors of the Major Salivary Glands pp. 16–39. Washington: Armed Forces Institute of Pathology.
Thiéry, J.-P. (1967) Mise en évidence des polysaccharides sur coupes fines en microscopie électronique.J. Microscopie 6, 987–1018.
Toolb, B. P. (1981) Glycosaminoglycans in morphogenesis InCell Biology of Extracellular Matrix (edited byHay, E. D.) pp. 259–94. New York: Plenum.
Yaku, Y. (1983) Ultrastructural studies on the development of human fetal salivary glands.Arch. Histol. Jpn. 46, 677–90.