Morphometric evaluation of wound healing in burns treated with Ulmo (Eucryphia cordifolia) honey alone and supplemented with ascorbic acid in guinea pig (Cavia porcellus)

Carolina Schencke1,2, Adriana Vasconcellos3, Cristian Sandoval1,4, Paulina Torres5, Francisca Acevedo5, Mariano del Sol3,6
1Doctoral Program in Morphological Sciences, Universidad de La Frontera, Temuco, Chile
2CONICYT-PCHA/National Doctorate Programme/2014-21141130 Santiago, Chile
3Facultad de Medicina, Universidad de la Frontera, Temuco, Chile
4CONICYT-PCHA/Doctorado Nacional/2015-21150991 Santiago, Chile
5Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Temuco, Chile
6Centro de Investigación en Ciencias Biomédicas Universidad Autónoma de Chile Temuco, Chile

Tóm tắt

AbstractBackgroundIn the context of the search for cost-efficient treatments, Ulmo (Eurcyphia cordifolia) honey is an excellent alternative for treating burn wounds and could have a profound medical, social, and economic impact. Ascorbic acid is an enzymatic co-factor necessary for the synthesis of collagen and the proliferation of fibroblasts and has been proposed as a coadjuvant to strengthen the healing effects of honey. The aim of this work was to evaluate by morphometric studies the healing wounds caused by burns treated with Ulmo honey alone and supplemented with ascorbic acid in guinea pig (Cavia porcellus).MethodsFifteen guinea pigs were used and divided randomly into three groups: positive control (C+), experimental with unsupplemented honey (H), and experimental with supplemented honey (SH). A uniform deep burn covering 1 cm2 of the back skin was performed. The following indices were calculated for the morphometric study: superficial contraction index of the wound, deep contraction index of the wound, wound severity index, global healing index, global contraction index, and dermal proliferation area.ResultsThe superficial contraction index of the wound, global healing index, global contraction, and dermal proliferation area values of the experimental with supplemented honey group were higher than the other groups (P < 0.05).ConclusionsAccording to these results, the combination of honey with an antioxidant (ascorbic acid) promotes an appropriate action to support the healing effect. This study showed that by supplementing the Ulmo honey with ascorbic acid, the healing and contraction effects can be strengthened in burn wounds compared to unsupplemented honey. These results were proof of the synergy between honey and ascorbic acid in healing burn wounds.

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