Morphological and molecular characterization of a new species Myxobolus gutturocola n. sp. (Myxozoa: Myxobolidae) from the throat of Hypophthalmichthys molitrix in China
Parasitology Research - 2019
Tóm tắt
Myxobolus gutturocola n. sp. was isolated from the throat of silver carp, Hypophthalmichthys molitrix, in Chongqing, China. Myxospore valves are unsymmetrical and smooth. Mature spores are ellipsoidal in frontal view, measuring 12.5 ± 0.2 μm (n = 25) in length, 8.4 ± 0.2 μm (n = 25) in width and 7.1 ± 0.2 μm (n = 25) in thickness. Each spore has two pyriform and unequal sizes polar capsules, the large one with 5.7 ± 0.2 μm in length × 3.6 ± 0.2 μm in width and the small one with 4.6 ± 0.2 μm in length × 2.6 ± 0.1 μm in width. Polar filaments are coiled seven or eight turns in the large polar capsule and four or five turns in the small polar capsule. The coils are arranged almost perpendicularly to the longitudinal axis of the polar capsule. Morphological analysis revealed that M. gutturocola n. sp. is distinct from related species of Myxobolus Bütschli, 1882. Molecular analysis has demonstrated that its SSU rDNA sequences do not match with any available sequences in GenBank. Phylogenetic analysis of the SSU rDNA sequences indicated this species clustered in a clade composed exclusively of parasites infecting the fishes of the Leucisini lineage and most closely related to Myxobolus pavlovskii isolated from the gill filaments of silver carp in Hungary.
Từ khóa
Tài liệu tham khảo
Abdel-Ghaffar F, Morsy K, El-Ganainy S, Ahmed M, Gamal S, Bashtar AR, Al-Quraishy S, Mehlhorn H (2015) Twelve myxosporean species of the family Myxobolidae infecting freshwater fishes of the River Nile, Egypt, with the description of four novel species. Parasitol Res 114:2985–2998
Abdel-Ghaffar F, Abdel-Gaber R, Maher S, El-Deeb N, Kamel R, Al-Quraishy S, Mehlhorn H (2017) Morphological and ultrastructural characteristics of Myxobolus ridibundae n. sp. (Myxosporea: Bivalvulida) infecting the testicular tissue of the marsh frog Rana ridibunda (Amphibia: Ranidae) in Egypt. Parasitol Res 116:133–141
Akhmerov AK (1954) Species complex of a new genus of Myxosporidia. Dok1 Akad.Nauk. SSSR, 97(6):1101–1103 (in Russian)
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410
Andree KB, Székely C, Molnár K, Gresoviac SJ, Hedrick RP (1999) Relationships among members of the genus Myxobolus (Myxozoa: Bilvalvidae) based on small subunit ribosomal DNA sequences. J Parasitol 85: 68–74
Atkinson SD, Bartošová-Sojková P, Whipps CM, Bartholomew JL (2015) Approaches for characterising myxozoan species. In: Myxozoan evolution, ecology and development. Springer International Publishing, pp 111–123
Barta JR, Martin DS, Liberator PA, Dashkevicz M, Anderson JW, Feighner SD, Elbrecht A, Perkins-Barrow A, Jenkins MC, Danforth HD, Ruff MD, Profous-Juchelka H (1997) Phylogenetic relationships among eight Eimeria species infecting the domestic fowl inferred using complete small subunit ribosomal DNA sequences. J Parasitol 83:262–271
Bartošová P, Fiala I (2011) Molecular evidence for the existence of cryptic species assemblages of several myxosporeans (myxozoa). Parasitol Res 108:573–583
Bartošová-Sojková P, Hrabcová M, Pecková H, Patra S, Kodádková A, Jurajda P, Tyml T, Holzer AS (2014) Hidden diversity and evolutionary trends in malacosporean parasites (Cnidaria: Myxozoa) identified using molecular phylogenetics. Int J Parasitol 44:565–577
Burland TG (2000) DNASTAR’s lasergene sequence analysis software. Methods Mol Biol 132:71–91
Carriero MM, Adriano EA, Silva MR, Ceccarelli PS, Maia AA (2013) Molecular phylogeny of the Myxobolus and henneguya genera with several new South American species. PLoS One 8:1–12
Chen CL, Ma CL (1998) Fauna Sinica: Myxozoa, Myxosporea. Science Press, Beijing, pp 1–806 (in Chinese)
Chen W, Yang CZ, Zhao YJ (2016) Molecular evolution of Myxobolus (Myxosporea) and coevolution with their hosts. Sichuan J Zool 35:183–189 (In Chinese with English abstract)
Cone DK, Yang J, Sun G, Easy R (2005) Taxonomy and molecular phylogeny of myxobolus bilobus n. sp. (myxozoa) parasitizing note migonuscry soleucas (cyprinidae) in Algonquin Park, Ontario, Canada. Dis Aquat Org 66:227–232
Dogiel VA, Akhmerov AK (1960) Myxosporidia of fishes of the Amur River Basin. RybnKhoz Vnutr Vodoemov Latv SSSR 5:239–308 (In Russian)
Easy RH, Johnson SC, Cone DK (2005) Morphological and molecular comparison of Myxobolus procerus (kudo, 1934) and M. intramusculi n. sp. (myxozoa) parasitising muscles of the trout-perch percopsis omiscomaycus. Syst Parasitol 61:115–122
Eiras JC, Molnár K, Lu YS (2005) Synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea: Myxobolidae). Syst Parasitol 61:1–46
Eiras JC, Zhang JY, Molnár K (2014) Synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea, Myxobolidae) described between 2005 and 2013. Syst Parasitol 88:11–36
Ferguson JA, Atkinson SD, Whipps CM, Kent ML (2008) Molecular and morphological analysis of Myxobolus spp. of salmonid fishes with the description of a new Myxobolus species. J Parasitol 94:1322–1334
Fiala I (2006) The phylogeny of Myxosporea (myxozoa) based on small subunit ribosomal RNA gene analysis. Int J Parasitol 36:1521–1534
Gunter NL, Adlard RD (2009) Seven new species of Ceratomyxa Thélohan, 1892 (Myxozoa) from the gall-bladders of serranid fishes from the Great Barrier Reef, Australia. Syst Parasitol 73:1–11
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
Hartikainen H, Gruhl A, Okamura B (2014) Diversification and repeated morphological transitions in endoparasitic cnidarians (Myxozoa: malacosporea). Mol Phylogenet Evol 76:261–269
Huang M, Liu Y, Jia L, Zhai YH, Deng Q, Gu ZM (2014) Supplementary studies on Myxobolus tsangwuensis Chen, 1954 (Myxozoa: myxosporea) infecting the gills of common carp Cyprinus carpio (L.): molecular and histological data. Acta Parasitol 59:653–660
Kent ML, Andree KB, Bartholomew JL, El-Matbouli M, Desser SS, Devlin RH, Feist SW, Hedrick RP, Hoffmann RW, Khattra J, Hallett SL, Lester RJ, Longshaw M, Palenzeula O, Siddall ME, Xiao C (2001) Recent advances in our knowledge of the Myxozoa. J Eukaryot Microbiol 48:395–413
Kong XH, Wang XZ, Gan XN, Li JB, He SP (2007) Phylogenetic relationships of Cyprinidae (Teleostei: Cypriniformes) inferred from the partial s6k1 gene sequences and implication of indel sites in intron 1. Sci China Life Sci 50:780–788
Landsberg JH, Lom J (1991) Taxonomy of the genera of the Myxobolus/Myxosoma group (Myxobolidae: Myxosporea), current listing of species and revision of synonyms. Syst Parasitol 18:165–180
Li LX, Nie DS (1973) Myxoporidia. In: Hubei Provincial Research Institute of Aquatic Biology (ed.) The Atlas of Pathogeny of Fish Disease in the Region of Hubei Province. Published by: Science Publishing House, Peking (China). pp 57–93 (In Chinese)
Liu Y, Whipps CM, Gu ZM, Zeng LB (2010) Myxobolus turpisrotundus (myxosporea: bivalvulida) spores with caudal appendages: investigating the validity of the genus Henneguya with morphological and molecular evidence. Parasitol Res 107:699–706
Liu XC, Yang CZ, Zhao YJ (2016) Redescription of Myxobolus honghuensis Liu et al. 2012 and identification on its genetic related species. Acta Hydrobiol Sin 40:352–359
Liu XH, Yuan S, Zhao YL, Fang P, Chen H, Zhang JY (2016a) Morphological and molecular characterization of Myxobolus sheyangensis n. sp. (Myxosporea: Myxobolidae) with intralamellar sporulation in allogynogeneticgibel carp, Carassius auratus gibelio (Bloch) in China. Parasitol Res 115:3567–3574
Liu XH, Batueva MD, Zhao YL, Zhang JY, Zhang QQ, Li TT, Li AH (2016b) Morphological and molecular characterisation of Myxobolus pronini n. sp. (Myxozoa: Myxobolidae) from the abdominal cavity and visceral serous membranes of the gibel carp Carassius auratus gibelio (Bloch) in Russia and China. Parasit Vectors 9:562–572
Liu XH, H CJ, Zhang QQ, Zhao YL, Zhang D, Zhang JY (2017) Myxobolus taibaiensis sp. n. (Myxozoa: Myxosporea) infecting the intestinal wall of common carp Cyprinus carpio Linnaeus in China. Folia Parasitol 64:1–7
Lom J, Arthur JR (1989) A guideline for the preparation of species descriptions in Myxosporea. J Fish Dis 12:151–156
Lom J, Dyková I (2006) Myxozoan genera: definition and notes on taxonomy, life-cycle terminology and pathogenic species. Folia Parasitol 53:1–36
Lom J, Molnár K (1983) Myxobolus basilamellaris sp. n. (Myxozoa: Myxosporea), a parasite of the gills of common carp (Cyprinus carpio L.). Folia Parasitol 30:1–3
Manrique WG, Figueiredo MA, de Andrade Belo MA, Martins ML, de Moraes FR (2015) First report of Myxobolus sp. infection in the skeletal muscle of Neotropical freshwater fish Piaractus mesopotamicus. Parasitol Res 114:2041–2044
Molnár K, Eszterbauer E, Székely C, Dán Á, Harrach B (2002) Morphological and molecular biological studies on intramuscular Myxobolus spp. of cyprinid fish. J Fish Dis 25:643–652
Molnár K, Marton S, Eszterbauer E, Székely C (2006) Comparative morphological and molecular studies on Myxobolus spp. infecting chub from the River Danube, Hungary, and description of M. muellericus sp. n. Dis Aquat Org 73:49–61
Molnár K, Székely C, Hallett SL, Atkinson SD (2009) Some remarks on the occurrence, host-specificity and validity of Myxobolus rotundus Nemeczek, 1911 (Myxozoa: Myxosporea). Syst Parasitol 72:71–79
Ran J, Yang CZ, Zhao YJ (2014) Taxonomic research of the myxosporidia based on genetic distance. J Chongqing Normal Univ 31:31–34
Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574
Schlegel M, Lom J, Stechmann A, Bernhard D, Leipe D, Dyková I, Sogin ML (1996) Phylogenetic analysis of complete small subunit ribosomal RNA coding region of Myxidium lieberkuehni: evidence that myxozoa are metazoa and related to the Bilateria. Arch Protistenkd 147:1–9
Shulman SS (1966) The myxosporidian fauna of the USSR. Nauka Publs, Moscow and Leningrad, pp 1–504 (translated by Sharma S. 1990)
Stamatakis A (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312–1313
Székely C, Molnár K, Cech G (2015) Description of Myxobolus balatonicus n. sp. (Myxozoa: Myxobolidae) from the common carp Cyprinus carpio L. in Lake Balaton. Syst Parasitol 91:71–79
Wang XZ, Li JB, He SP (2007) Molecular evidence for the monophyly of East Asian groups of Cyprinidae (Teleostei: Cypriniformes) derived from the nuclear recombination activating gene 2 sequences. Mol Phylogenet Evol 42:157–170
Wang XZ, Gan XN, Li JB, Mayden RL, He SP (2012) Phylogenetic analysis and systematic significance of Cyprinidae (Teleostei: Cypriniformes) based on Bayesian method and maximum likelihood method. Sci China Life Sci 42:648–661
Whipps CM, Kent ML (2006) Phylogeography of the cosmopolitan marine parasite Kudoathyrsites (Myxozoa: Myxosporea). J Eukaryot Microbiol 53:364–373
Whipps CM, Zhao YJ (2015) Synopsis of the species of the genus Sphaeromyxa, Thélohan, 1892 (Myxosporea: Bivalvulida: Variisporina: Sphaeromyxidae). Syst Parasitol 92:81–99
Yakovchuk TA (1979) A new species of the genus Myxobolus (Myxosporidia: Myxobolidae) from the gill filaments of the carp. Parazitologiia 13:635–636 (In Russian)
Zhang JY, Wang JG, Li AH, Gong XN (2010) Infection of Myxobolus turpisrotundus sp. n. in allogynogenetic gibel carp, Carassius auratus gibelio (Bloch), with revision of Myxobolus rotundus (s. l.) Nemeczek reported from C. auratus auratus (l.). J Fish Dis 33:625–638
Zhang B, Gu ZM, Liu Y (2018) Morphological, histological and molecular characterization of three Myxobolus species (Cnidaria: Myxosporea) from silver carp Hypophthalmichthys molitrix Valenciennes and bighead carp Hypophthalmichthys nobilis Richardson in China. Parasitol Int 67:509–516
Zhao YJ, Ma CL, Song WB (2001) Illustrated guide to the identification of pathogenetic protozoa in maricuture—II diagnostic methods for the myxosporean. J Ocean Univ Qingdao 31:681–688
Zhao YJ, Sun CY, Kent ML, Deng JL, Whipps CM (2008) Description of a new species of Myxobolus (Myxozoa: Myxobolidae) based on morphological and molecular data. J Parasitol 94:737–742
Zhao YJ, Li NN, Tang FH, Dong JL (2013) Remarks on the validity of Myxobolus ampullicapsulatus and Myxobolus honghuensis (Myxozoa: Myxosporea) based on SSU rDNA sequences. Parasitol Res 112:3817–3823