Morphologic and cytochemical identification of microperoxisomes in human and non-human primate placental trophoblast

Springer Science and Business Media LLC - Tập 74 - Trang 115-121 - 1982
B. F. King1
1Department of Human Anatomy, University of California School of Medicine, Davis, USA

Tóm tắt

Human and non-human primate placental villi (near term) were incubated in an alkaline 3,3′ diamino benzidine (DAB) medium to identify the distribution of microperoxisomes in these tissues. Catalase-positive microperoxisomes were present in small numbers in cytotrophoblast, but were virtually absent from syncytiotrophoblast. Microperoxisomes were roughly circular in shape and about 0.2–0.3 μm in diameter. They always had a close association with an element of agranular endoplasmic reticulum. The function of microperoxisomes in placenta, as in many other tissues, is uncertain. What-ever their function, they are presumably more important in cytotrophoblast metabolism than in syncytium.

Tài liệu tham khảo

Black VH, Bogart BI (1973) Peroxisomes in inner adrenocortical cells of fetal and adult guinea pigs. J Cell Biol 57:345–358 deDuve C (1973) Biochemical studies on the occurrence, biogenesis and life history of mammalian peroxisomes. J Histochem Cytochem 21:941–948 Familiari G, Franchitto G, Correr S, Motta P (1979) Microperoxisomes in steroidogenic cells of the rat ovary-interstitial, thecal and luteal cells. Experientia 35:1503–1505 Gemmell RT, Stacy BD (1979) Ultrastructural study of granules in the corpora lutea of several mammalian species. Am J Anat 155:1–14 Gulyas BJ, Yuan LC (1975) Microperoxisomes in the late pregnancy corpus luteum of rhesus monkeys (Macaca mulatta). J Histochem Cytochem 23:359–368 Gulyas BF, Yuan LC (1977) Association of microperoxisomes with the endoplasmic reticulum in granulosa lutein cells of the rhesus monkey. Cell Tissue Res 179:357–366 Hand AR (1973) Morphologic and cytochemical identification of peroxisomes in the rat parotid and other exocrine glands. J Histochem Cytochem 21:131–141 Herzog V, Fahimi HD (1974) Microbodies (peroxisomes) containing catalase in myocardium: morphological and biochemical evidence. Science 185:271–273 Herzog V, Fahimi HD (1975) Identification of peroxisomes (microbodies) in mouse myocardium. J Mol Cell Cardiol 8:271–273 Hruban Z, Rechncigl M (1969) Microbodies and related particles. Int Rev Cytol Suppl 1:1–296 Kodr GS, Siler-Kodr T (1978) Localization of luteinizing hormone-releasing factor in the human placenta. Fertil Steril 29:523–526 Lazarow PB (1978) Rat liver peroxisomes catalyze the β-oxidation of fatty acids. J Biol Chem 253:1522–1528 Lazarow PB, deDuve C (1976) A fatty acyl-CoA oxidizing system in rat liver peroxisomes: Enhancement by clofibrate, a hypolipidemic drug. Proc Natl Acad Sci USA 73:2043–2046 Lin TJ, Lin SL, Erlenmeyer F, Line IT, Underwood R, Billiar RB, Little B (1972) Progesterone production rates during the third trimester of pregnancy in normal women, diabetic women, and women with abnormal glucose tolerance. J Clin Endocrinol Metab 34:287–297 Maniscalco W, Warshaw JB (1978) Cellular energy metabolism during fetal and perinatal development. In: Sinclair JC (ed) Temperature regulation and energy metabolism in the newborn. Grune & Stratton, New York, pp 1–38 Margoliash E, Novogrodsky A, Schejter A (1960) Irreversible reaction of 3-amino-1:2:4-triazole and related inhibitors with the protein of catalase. Biochem J 74:339–350 Masters C, Holmes R (1977) Peroxisomes: New aspects of cell physiology and biochemistry. Physiol Rev 57:816–882 Nelson DM, Enders AC, King BF (1978a) Cytological events involved in protein synthesis in cellular and syncytial trophoblast of human placenta. An electron microscope autoradiographic study of (3H) leucine incorporation. J Cell Biol 76:400–417 Nelson DM, Enders AC, King BF (1978b) Cytological events involved in glycoprotein synthesis in cellular and syncytial trophoblast of human placenta: An electron microscope autoradiographic study of (3H) galactose incorporation. J Cell Biol 76:418–429 Novikoff AB, Novikoff PM, Davis C, Quintana N (1972) Studies on microperoxisomes. II. A cytochemical method for light and electron microscopy. J Histochem Cytochem 20:1006–1023 Novikoff AB, Novikoff PM, Quintana N, Davis C (1973a) Studies on microperoxisomes. IV. Interrelations of microperoxisomes, endoplasmic reticulum and lipofuscin granules. J Histochem Cytochem 21:1010–1020 Novikoff PM, Novikoff AB, Davis C, Quintano N (1973b) Studies on microperoxisomes. V. Are microperoxisomes ubiquitous in mammalian cells? J Histochem Cytochem 21:373–755 Paavola LG, Boyd CO (1981) Cytoplasmic granules in luteal cells of pregnant and non-pregnant guinea pigs. A cytochemical study. Anat Rec 201:127–140 Reddy J, Svoboda D (1972a) Microbodies (peroxisomes) identification in interstitial cells of the testis. J Histochem Cytochem 20:140–142 Reddy J, Svoboda D (1972b) Microbodies (peroxisomes) in the interstitial cells of rodent testes. Lab Invest 26:657–665 Russo JJ, Black VH (1980) Peroxisomes in guinea pig adrenocortical cells: Enzymatic characterization and responsiveness to adrenocorticotrophic hormone (ACTH). J Cell Biol 87:168a Russo JJ, Black VH (1981) Isolation of a peroxisome-enriched fraction from the guinea pig adrenal. Anat Rec 199:221A Tolbert NE (1981) Metabolic pathways in peroxisomes and glyoxysomes. Annu Rev Biochem 50:133–157 Warshaw JB (1981) Control of fatty acid metabolism during development. In: Physiological and biochemical basis for perinatal medicine. S Karger, Basel, pp 101–107