Morphogenetic potential of native passion fruit (Passiflora gibertii N. E. Brown.) calli
Tóm tắt
Some species of non-cultivated passion fruit plant have important contributions to genetic improvement. However, there are few studies concerning about embryogenic and organogenic calli mainly related with structural alterations during their development. The objective of this work was to characterize, structurally the callogenesis on leaf explants of Passiflora gibertii N. E. Brown. The cotyledons were inoculated in MS culture medium, containing half salt concentration, supplemented with sucrose (3 %), and picloram+kinetin for the calli formation. Different calli colors were obtained and used for structural analyses. The calli colors were translucent, white, dark-yellow, white-brown, light-yellow, and white-yellow. After 30 days of cultivation, the calli were fixed in Karnovsky and prepared for the visualization under the scanning and transmission electron microscope and optic microscope. Translucent and light-yellow calli did not present morphogenic characteristics. The cells had different shapes forming non-organized cellular system and the absence or reduced starch content. On the other hand, white and dark-yellow calli manifested embryogenic characteristics such as small isodiametric cells, an organized cellular, dense cytoplasm rich in mitochondria and endoplasmic reticulum, small vacuole and significant starch contend. The culture medium supplemented with 4.14 μM of picloram +0.46 μM of kinetin is the most suitable to induce embryogenic cells.
Tài liệu tham khảo
Cangahuala-Inocente GC, Steiner N, Santos M, Guerra MP (2004) Morphological analysis and histochemistry of Feijoa sellowiana somatic embryogenesis. Protoplasma 224:3340
Canhoto JM, Mesquita JF, Cruz GS (1996) Ultrastructural changes in cotyledons of pineapple guava (Myrtaceae) during somatic embryogenesis. Ann Bot 78:513–521
Carvalho DC, Siva ALL, Tanno GT, Purcino M, Bias LA (2011) Organogênese a partir de segmentos foliares e internodais de videira cv. Merlot. Ciênc Agrotec 35:108–114
Chen AH, Yang JL, Niu YD, Yang CP, Liu GF, Yu CY, Li CH (2010) High-frequency somatic embryogenesis from germinated zygotic embryos of Schisandra chinensis and evaluation of the effects of medium strength, sucrose, GA3, and BA on somatic embryo development. Plant Cell Tiss Org Cult 102:357–364
Cunha MAP, Barbosa LV, Junqueira NTV (2002) Espécies de maracujazeiro. In: Lima AA (ed) Maracujá produção: aspectos técnicos. Embrapa Informação Tecnológica, Brasília, p 104
Fehér A (2005) Why somatic plant cells start to form embryos? In: Mujib A, Samaj J (eds) Somatic embryogenesis., pp 85–101
Fehér A, Pasternak T, Dudits D (2003) Transition of somatic plant cells to an embryogenic state. Plant Cell Tiss Organ Cult 74:201–228
Fernando JA (1999) Estudos anatômicos da embriogênese somática in vitro em soja (Glycine max (L.) Merrill). Dissertação de Mestrado. Piracicaba USP/ESALQ, p 60
Fernando JA, Melo M, Soares MKM, Appezzato-da-Glória B (2001) Anatomy of somatic embryogenesis in Carica papaya L. Braz Arch Biol Technol 44:247–255
Fernando SC, Verdeil JL, Hocher V, Weerakoon LK, Hirimburegama K (2003) Histological analysis of plant regeneration from plumule explants of Cocos nucifera. Plant Cell Tiss Org Cult 72:281–284
Figueiredo SFL, Simões C, Albarello N, Viana VRC (2000) Rollinia mucosa cell suspension cultures: establishment and growth conditions. Plant Cell Tiss Org Cult 63:85–92
Filonova Lh, Bozhkov Pv, Brukhin Vb, Daniel G, Zhivotovsky B, Von Arnold S (2000) Two waves of programmed cell death occur during formation and development of somatic embryos in the gymnosperm, Norway spruce. J Cell Sci 113:4399–4411
Fischer IH (2003) Seleção de plantas resistentes e de fungicidas para o controle da “morte prematura” do maracujazeiro, causada por Nectria haematococca e Phytophthora parasitica. Dissertação de Mestrado. Piracicaba USP/ESALQ, p 48
George EF (1993) Plant propagation by tissue culture—the technology. Exegetics, Edington
Meletti LMM, Bruckner CH (2001) Melhoramento genético. In: Bruckner CH, Picanço MC (eds) Maracujá: tecnologia de produção, pós-colheita, agroindústria, mercado., pp 345–385
Mikuła A, Tykarska T, Kuras M, Rybczyński JJ (2005) Somatic embryogenesis of Gentiana cruciata (L.): histological and ultrastructural changes in seedling hypocotyl explant. In Vitro Cell Dev Biol 41:686–694
Monteiro ACBA, Higashi EN, Gonçalves AN, Rodriguez APM (2000) A novel approach for the definition of the inorganic medium component for micropropagation of yellow passion fruit (Passiflora edulis Sims. f. flavicarpa Deg.). In Vitro Cell Dev Biol 36:527–531
Moura Barros L (1999) Embriogênese somática. Biotec Ciênc e Desenvol 2:36–43
Moura EF, Ventrella MC, Motoike SY (2010) Anatomy, histochemistry and ultrastructure of seed and somatic embryo of Acrocomia aculeata (Arecaceae). Sci Agric 67:399–407
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nogueira RC, Paiva R, Porto JMP, Nicioli PM, Stein VC, Deuner S, Alves E (2007) Análise ultra-estrutural de calos embriogênicos de murici-pequeno (Byrsonima intermedia A. Juss.). Rev Bras de Bioci 5:48–50
Oliveira Ribeiro L, Paiva LV, Pádua, Santos BR, Alves E, Stein VC (2012) Morphological and ultrastructural analysis of various types of banana callus, cv. Prata anã. Acta Sci, Agron 3:423–429
Palmer CD, Keller WA (2011) Somatic embryogenesis in Crambe abyssinica Hochst. ex R. E. Fries using seedling explants. Plant Cell Tiss Org Cult 104:10–91
Pihakashi-Maunsbach K, Nygaard KB, Jensen KH, Rasmussen (1993) O Cellular changes in early development of regenerating thin cell layer-explants of rapeseed analysed by light and electron microscopy. Physiol Plant 87:167–176
Portillo L, Olmedilla A, Santacruz-Ruvalcaba F (2012) Cellular and molecular changes associated with somatic embryogenesis induction in Agave tequilana. Protoplasma 4:1101–1107
Prakash MG, Gurumurthi K (2010) Effects of type of explant and age, plant growth regulators and medium strength on somatic embryogenesis and plant regeneration in Eucalyptus camaldulensis. Plant Cell Tiss Org Cult 100:2–13
Rocha DI, Vieira LM, Tanaka FAO, da Silva LC, Otoini WC (2012) Somatic embryogenesis of a wild passion fruit species Passiflora cincinnata Masters: histocytological and histochemical evidences. Protoplasma 249:747–758
Roncatto G, Oliveira JC, Ruggiero C, Filho GCN, Centurion MAPC, Ferreira FR (2004) Comportamento de maracujazeiros (Passiflora spp.) quanto à morte prematura. Rev Bras Frutic 26:552–554
Rosal LF (2004) Germinação, indução de calos, micropropagação e anatomia foliar da candeia (Eremanthus erythropappus (DC.) Mac Leish). Dissertação de Mestrado. Lavras. UFLA
Rowley CR, Moran DT (1975) A simple procedure for mounting wring wrinkle—free sections on formvar—coated slot grids. Ultramicrotomy 1:151–155
Sané D, Aberlenc-Bertossi F, Gassama-Dia YK, Sagna M, Trouslot MF, Duval Y, Borgel A (2006) Histocytological analysis of callogenesis and somatic embryogenesis from cell suspensions of date palm (Phoenix dactylifera). Ann Bot 98:301–308
Santana JRF, Paiva R, Souza AV, Oliveira LM (2011) Effect of different culture tube caps and concentrations of activated charcoal and sucrose on in vitro growth and budding induction of Annona glabra L. Ciênc Agrotec 35:916
Schumann G, Ryschika U, Schulze J, Klocke E (1995) Anatomy of somatic embryogenesis. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Springer, Berlin Heidelberg, pp 71–86
Shang H–H, Liu C-L, Zhang C, Li F-L, Hong W-D, Li F-G (2009) Histological and ultrastructural observation reveals significant cellular differences between Agrobacterium transformed embryogenic and non-embryogenic calli of cotton. J Integr Plant Biol 51:456–465
Silva Guedes R, Silva TL, Luis ZGL, Scherwinski-Pereira JE (2011) Initial requirements for embryogenic calluses initiation in thin cell layers explants from immature female oil palm inflorescences. Afr J Biotechnol 10:10774–10780
Silva Paula M, Noronha Fonseca ME, Boiteux LS, Peixoto JR (2010) Caracterização genética de espécies de Passiflora por marcadores moleculares análogos a genes de resistência. Rev Bras Frutic 32:222–229
Souza AV, Bertoni BW, Castro Franca S, Pereira AMS (2011) Micropropagação de Dioscorea multiflora Grised. Ciênc agrotec 35:92–98
Stein VC, Paiva R, Vargas DP, Soares F, Alves E, Nogueira GF (2010) Ultrastructural calli analysis of Inga vera Willd subsp affinis (DC) TD Penn. Rev Árvore 34:789–796
Steiner N, Vieira FN, Maldonado S, Guerra MP (2005) Carbon source affects morphogenesis and histodifferentiation of Araucaria angustifolia embryogenic cultures. Braz Arch Biol Technol 48:895–903
Stella A, Braga MR (2002) Callus and cell suspension cultures of Rudgea jasminoides, a tropical woody Rubiaceae. Plant Cell Tiss Org Cult 68:271–276
Villalobo AG, Justo SR, Rodríguez R (2012) Morpho-physiological changes in pineapple plantlets [Ananas comosus (L.) merr.] during acclimatization. Ciência e Agrotecnologia 36:624–630
Williams EG, Maheswaran G (1986) Somatic embryogenesis: factors influencing coordinated behavior of cells as an embryogenic group. Ann Bot 57:443–462
Zienkiewicz A, Jiménez-López JC, Zienkiewicz K, Alché JD, Rodríguez-García MI (2011) Development of the cotyledon cells during olive (Olea europaea L.) in vitro seed germination and seedling growth. Protoplasma 248:751–765