Monokine production by microglial cell clones

European Journal of Immunology - Tập 19 Số 8 - Trang 1443-1448 - 1989
Marco Righi1, Lucia Mori1, Gennaro De Libero1,2, Marina Sironi3, Andrea Biondi3, Alberto Mantovani3, Suzanne Denis Donini4, Paola Ricciardi‐Castagnoli5
1Department of Biology,Milano
2L. Mori was supported by the Italian Association for Cancer Research and G. De Libero by a CNR fellowship.
3CNR Center of Cytopharmacology, Institute "Mario Negri", Milano
4University of Milano, Milano
5CNR Center of Cytopharmacology, Via Vanvitelli, 32, I-20129 Milano, Italy

Tóm tắt

Abstract

Cytokines have been suggested to act as intermediates between the immune and the central nervous system, but little is known about the type of cells synthesizing them in the brain. We have immortalized with oncogenic retroviruses primary brain cell cultures from mouse embryos and have generated clones of microglial cells that have been characterized. Three of the clones studied produce interleukin 1 (IL 1), IL 6 and tumor necrosis factor‐α as assessed by biological assays and by Northern blot analysis. Our data raise the question on the role of these cytokines in the brain and suggest that early resident microglial cells might play an important role in developmental processes and in the adult brain.

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