Monitoring the occurrence of bacterial spot of tomato and pepper in the Czech Republic and development of new PCR primers for detection of Xanthomonas vesicatoria
Tóm tắt
We tested 212 bacterial isolates isolated from tomato and pepper plants suspected of being infected by Xanthomonas vesicatoria (causing bacterial spot). Samples from plants were collected in the years 2007–2010 from fields located in central Bohemia and central and south Moravia. For detection of the pathogens we used PCR with two previously published primer pairs (RST65, RST69 and XCVF, XCVR) and one primer pair (Xv1, Xv2) designed for specific detection of Xanthomonas vesicatoria. In total, 121 of 212 (57 %) samples were positive in PCR. In this article we provide data for additional verification of the newly designed (Xv1, Xv2) primers. Our results confirm the need of precise detection methods, because several tomato and pepper pathogens can share similar visual symptoms. We also suggest, that new PCR primers for detection of bacterial spot of tomato and pepper pathogens should not be overlooked, because they can improve currently used PCR assays for detection of these pathogens.
Tài liệu tham khảo
Al-Dahmani, J. H., Abbasi, P. A., Miller, S. A., & Hoitink, H. A. J. (2003). Suppression of bacterial spot of tomato with foliar sprays of compost extracts under greenhouse conditions. Plant Disease, 87, 913–919.
Araújo, E. R., Ferreira, M. A. S. V., & Quezado-Duval, A. M. (2013). Specific primers for Xanthomonas vesicatoria, a tomato bacterial spot causal agent. European Journal of Plant Pathology, 137, 5–9.
Beran, P., & Mráz, I. (2013). Species-specific PCR primers for detection of Xanthomonas vesicatoria. Crop Protection, 43, 213–215.
Doidge, E. M. (1920). A tomato cancer. Journal of the Department of Agriculture, Union of South Africa., 1, 718–721.
Dominguez, M. C., de La Rosa, M., & Borobio, M. V. (2001). Application of a spectrophotometric method for the determination of post-antibiotic effect and comparison with viable counts in agar. Journal of Antimicrobial Chemotherapy, 47, 391–398.
Jones, J. B., Stall, R. E., & Bouzar, H. (1998). Diversity among Xanthomonads pathogenic on pepper and tomato. Annual Review of Phytopathology, 36, 41–58.
Jones, J. B., Lacy, G. H., Bouzar, H., Stall, R. E., & Schaad, N. W. (2004). Reclassification of the xanthomonads associated with bacterial spot disease of tomato and pepper. Systematic and Applied Microbiology, 27, 755–762.
Jones, J. B., Lacy, G. H., Bouzar, H., Stall, R. E., & Schaad, N. W. (2006). Reclassification of the xanthomonads associated with bacterial spot disease of tomato and pepper. Systematic and Applied Microbiology. Erratum: Systematic and Applied Microbiology, 29, 85–86.
Koenraadt, H., van Betteray, B., Germain, R., Hiddink, G., Jones, J. B., & Oosterhof, J. (2009). Development of specific primers for the molecular detection of bacterial spot of pepper and tomato. ISHS Acta Horticulturae, 808, 99–102.
Leite, R. P., Jr., Minsavage, G. V., Bonas, U., & Stall, R. E. (1994). Detection and identification of phytopathogenic Xanthomonas strains by amplification of DNA sequences related to the hrp genes of Xanthomonas campestris pv. vesicatoria. Applied and Environmental Microbiology, 60, 1068–1077.
Moretti, C., Amatulli, M. T., & Buonaurio, R. (2009). PCR-based assay for the detection of Xanthomonas euvesicatoria causing pepper and tomato bacterial spot. Letters in Applied Microbiology, 49, 466–471.
Obradovic, A., Mavridis, A., Rudolph, K., Janse, J., Arsenijevic, M., Jones, J., Minsavage, G., & Wang, J. (2004). Characterization and PCR-based typing of Xanthomonas campestris pv. vesicatoria from peppers and tomatoes in Serbia. European Journal of Plant Pathology, 110, 285–292.
Park, D. S., Shim, J. K., Kim, J. S., Lim, C. K., Shrestha, R., Hahn, J. H., & Kim, H. G. (2009). Sensitive and specific detection of Xanthomonas campestris pv. vesicatoria by PCR using pathovar-specific primers based on rhs family gene sequences. Microbiological Research, 164, 36–42.
Potnis, N., Krasileva, K., Chow, V., Almeida, N. F., Patil, P. B., Ryan, R. P., Sharlach, M., Behlau, F., Dow, J. M., Momol, M., White, F. F., Preston, J. F., Vinatzer, B. A., Koebnik, R., Setubal, J. C., Norman, D. J., Staskawicz, B. J., & Jones, J. B. (2011). Comparative genomics reveals diversity among xanthomonads infecting tomato and pepper. BMC Genomics, 12, 146.
Sutic, D. (1957). Bakterioze crvenog patlidzana (Tomato bacteriosis). Posebna Izdanja Institut za zastitu bilja Beograd (Special Edition Institute for Plant Protection Belgrade) 6, 1–65. English Summary Review of Applied Mycology, 36, 734–735.
Vauterin, L., Hoste, B., Kersters, K., & Swings, J. (1995). Reclassification of Xanthomonas. International Journal of Systematic Bacteriology, 45, 472–489.