Monitoring the expression profiles of 7000 <i>Arabidopsis</i> genes under drought, cold and high‐salinity stresses using a full‐length cDNA microarray

Plant Journal - Tập 31 Số 3 - Trang 279-292 - 2002
Motoaki Seki1,2,3, Mari Narusaka2,3, Junko Ishida2, Tokihiko Nanjo4,1, Miki Fujita2, Youko Oono1,5, Asako Kamiya2, Maiko Nakajima2, Akiko Enju2, Tetsuya Sakurai2, Masakazu Satou2, Kenji Akiyama2, Teruaki Taji1,5, Kazuo Shinozaki6, Piero Carninci7, Jun Kawai8,7, Yoshihide Hayashizaki8,7, Kazuo Shinozaki1,2
1Laboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan
2Plant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3-1-1 Koyadai, Tsukuba 305-0074, Japan
3The first two authors contributed equally to this work.
4Genesis Research Institute, Inc, 4-1-35 Noritake-Shinmachi, Nishi-ku, Nagoya, Aichi, 451-0051, Japan
5Master's Program in Biosystem Studies, University of Tsukuba, Tennodai, Tsukuba, Ibaraki, 305-8572, Japan,
6Biological Resources Division, Japan International Research Center for Agricultural Sciences, Ministry of Agriculture, Forestry and Fisheries, 2-1 Ohwashi, Tsukuba, Ibaraki 305-0851, Japan
7Genome Science Laboratory, RIKEN Tsukuba Institute, 3-1-1 Koyadai, Tsukuba 305-0074, Japan,
8Genome Exploration Research Group, RIKEN Genomic Sciences Center, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan,

Tóm tắt

SummaryFull‐length cDNAs are essential for functional analysis of plant genes in the post‐sequencing era of the Arabidopsis genome. Recently, cDNA microarray analysis has been developed for quantitative analysis of global and simultaneous analysis of expression profiles. We have prepared a full‐length cDNA microarray containing ≈7000 independent, full‐length cDNA groups to analyse the expression profiles of genes under drought, cold (low temperature) and high‐salinity stress conditions over time. The transcripts of 53, 277 and 194 genes increased after cold, drought and high‐salinity treatments, respectively, more than fivefold compared with the control genes. We also identified many highly drought‐, cold‐ or high‐salinity‐ stress‐inducible genes. However, we observed strong relationships in the expression of these stress‐responsive genes based on Venn diagram analysis, and found 22 stress‐inducible genes that responded to all three stresses. Several gene groups showing different expression profiles were identified by analysis of their expression patterns during stress‐responsive gene induction. The cold‐inducible genes were classified into at least two gene groups from their expression profiles. DREB1A was included in a group whose expression peaked at 2 h after cold treatment. Among the drought, cold or high‐salinity stress‐inducible genes identified, we found 40 transcription factor genes (corresponding to ≈11% of all stress‐inducible genes identified), suggesting that various transcriptional regulatory mechanisms function in the drought, cold or high‐salinity stress signal transduction pathways.

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Plant Journal

Tập 31 Số 3

279-292

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