Molecular integrity and global gene expression of breast and lung cancer stem cells under long-term storage and recovery

Cell and Tissue Banking - Tập 14 - Trang 175-186 - 2012
Feridoun Karimi-Busheri1,2, Victoria Zadorozhny1, Ewa Carrier1, Habib Fakhrai1
1Stem Cell Department, NovaRx Corporation, San Diego, USA
2Department of Oncology, University of Alberta, Edmonton, Canada

Tóm tắt

Cryopreservation is a common procedure widely used in biological and clinical sciences. Similar protocols are also applied in preserving cancer stem cells, a field with high promises and challenges. Specific cell surface membrane proteins are considered to be biomarkers of cancer stem cells and they may play a critical role in differentiating stem cells from non stem cells. We have looked at the possible effect of long-term cryopreservation on the molecular integrity of breast MCF7 and lung, A549 and H460, cancer stem cells and to assess if these cells are more sensitive to long-term storage process. We analyzed the expression of CD24 and CD38 as two potent biomarkers of lung cancer stem cells and EpCAM and ALDH that are used as biomarkers of a wide range of cancer stem cells. We also selected three genes essential for the normal functioning of the cells, Fos, MUC1, and HLA. Our results indicate a pattern of down-regulation in the expression of the genes following freezing, in particular among cell surface marker proteins. Global gene expression of the post-thaw breast and lung cancer stem cells also reveals a significant down-regulation in freeze-thaw cells independent from each other. Analyzing the canonical pathways between two populations reveals a significant alteration in the gene expression of the pathways involved in cell cycle, mitosis, and ataxia telangiectasia mutated pathways. Overall, our results indicate that current protocols for long-term storage of lung and breast cancer stem cells may substantially influence the activity and function of genes.

Tài liệu tham khảo

Al-Hajj M, Wicha MS, Benito-Hernandez A, Morrison SJ, Clarke MF (2003) Prospective identification of tumorigenic breast cancer cells. Proc Natl Acad Sci USA 100:3983–3988 Alison MR, Guppy NJ, Lim SM, Nicholson LJ (2010) Finding cancer stem cells: are aldehyde dehydrogenases fit for purpose? J Pathol 222:335–344 Bafna S, Kaur S, Batra SK (2010) Membrane-bound mucins: the mechanistic basis for alterations in the growth and survival of cancer cells. Oncogene 29:2893–2904 Berz D, McCormack EM, Winer ES, Colvin GA, Quesenberry PJ (2007) Cryopreservation of hematopoietic stem cells. Am J Hematol 82:463–472 Bischof JC, Wolkers WF, Tsvetkova NM, Oliver AE, Crowe JH (2002) Lipid and protein changes due to freezing in Dunning AT-1 cells. Cryobiology 45:22–32 Charron D (2011) HLA, immunogenetics, pharmacogenetics and personalized medicine. Vox Sang 100:163–166 Chong YK, Toh TB, Zaiden N, Poonepalli A, Leong SH, Ong CE, Yu Y, Tan PB, See SJ, Ng WH, Ng I, Hande MP, Kon OL, Ang BT, Tang C (2009) Cryopreservation of neurospheres derived from human glioblastoma multiforme. Stem Cells 27:29–39 Eyler CE, Wu Q, Yan K, Macswords JM, Chandler-Militello D, Misuraca KL, Lathia JD, Forrester MT, Lee J, Stamler JS, Goldman SA, Bredel M, McLendon RE, Sloan AE, Hjelmeland AB, Rich JN (2011) Glioma stem cell proliferation and tumor growth are promoted by nitric oxide synthase-2. Cell 146:53–66 Gordon N, Kleinerman ES (2009) The role of Fas/FasL in the metastatic potential of osteosarcoma and targeting this pathway for the treatment of osteosarcoma lung metastases. Cancer Treat Res 152:497–508 Hermann PC, Huber SL, Herrler T, Aicher A, Ellwart JW, Guba M, Bruns CJ, Heeschen C (2007) Distinct populations of cancer stem cells determine tumor growth and metastatic activity in human pancreatic cancer. Cell Stem Cell 1:313–323 Karimi-Busheri F, Rasouli-Nia A, Mackey JR, Weinfeld M (2010a) Senescence evasion by MCF-7 human breast tumor-initiating cells. Breast Cancer Res 12:R31 Karimi-Busheri F, Zadorozhny V, Shawler DL, Fakhrai H (2010b) The stability of breast cancer progenitor cells during cryopreservation: maintenance of proliferation, self-renewal, and senescence characteristics. Cryobiology 60:308–314 Karimi-Busheri F, Zadorozhny V, Li T, Lin H, Shawler DL, Fakhrai H (2012) Pivotal role of CD38 biomarker in combination with CD24, EpCAM, and ALDH for identification of H460 derived lung cancer stem cells. Journal of Stem Cells 6:1 Kasprzycka M, Marzec M, Liu X, Zhang Q, Wasik MA (2006) Nucleophosmin/anaplastic lymphoma kinase (NPM/ALK) oncoprotein induces the T regulatory cell phenotype by activating STAT3. Proc Natl Acad Sci USA 103:9964–9969 Kuilman T, Michaloglou C, Mooi WJ, Peeper DS (2010) The essence of senescence. Genes Dev 24:2463–2479 Lang SH, Frame FM, Collins AT (2009) Prostate cancer stem cells. J Pathol 217:299–306 Leung EL, Fiscus RR, Tung JW, Tin VP, Cheng LC, Sihoe AD, Fink LM, Ma Y, Wong MP (2010) Non-small cell lung cancer cells expressing CD44 are enriched for stem cell-like properties. PLoS ONE 5:e14062 Levina V, Marrangoni AM, DeMarco R, Gorelik E, Lokshin AE (2008) Drug-selected human lung cancer stem cells: cytokine network, tumorigenic and metastatic properties. PLoS ONE 3:e3077 Lonardo E, Hermann PC, Heeschen C (2010) Pancreatic cancer stem cells—update and future perspectives. Mol Oncol 4:431–442 Malavasi F, Deaglio S, Funaro A, Ferrero E, Horenstein AL, Ortolan E, Vaisitti T, Aydin S (2008) Evolution and function of the ADP ribosyl cyclase/CD38 gene family in physiology and pathology. Physiol Rev 88:841–886 Manipalviratn S, Huang A, Decherney AH (2006) Future of infertility treatment. Int Surg 91:S3–S14 Mazur P (1970) Cryobiology: the freezing of biological systems. Science 168:939–949 Milas L, Hittelman WN (2009) Cancer stem cells and tumor response to therapy: current problems and future prospects. Semin Radiat Oncol 19:96–105 Mimeault M, Batra SK (2010) New advances on critical implications of tumor- and metastasis-initiating cells in cancer progression, treatment resistance and disease recurrence. Histol Histopathol 25:1057–1073 Monteiro J, Fodde R (2010) Cancer stemness and metastasis: therapeutic consequences and perspectives. Eur J Cancer 46:1198–1203 Moreb JS, Zucali JR, Ostmark B, Benson NA (2007) Heterogeneity of aldehyde dehydrogenase expression in lung cancer cell lines is revealed by Aldefluor flow cytometry-based assay. Cytometry B Clin Cytom 72:281–289 Muramoto GG, Russell JL, Safi R, Salter AB, Himburg HA, Daher P, Meadows SK, Doan P, Storms RW, Chao NJ, McDonnell DP, Chute JP (2010) Inhibition of aldehyde dehydrogenase expands hematopoietic stem cells with radioprotective capacity. Stem Cells 28:523–534 Notta F, Doulatov S, Laurenti E, Poeppl A, Jurisica I, Dick JE (2011) Isolation of single human hematopoietic stem cells capable of long-term multilineage engraftment. Science 333:218–221 O’Brien CA, Kreso A, Dick JE (2009) Cancer stem cells in solid tumors: an overview. Semin Radiat Oncol 19:71–77 Pardal R, Clarke MF, Morrison SJ (2003) Applying the principles of stem-cell biology to cancer. Nat Rev Cancer 3:895–902 Pegg DE (2002) The history and principles of cryopreservation. Semin Reprod Med 20:5–13 Rasouli-Nia A, Karimi-Busheri F, Weinfeld M (2004) Stable down-regulation of human polynucleotide kinase enhances spontaneous mutation frequency and sensitizes cells to genotoxic agents. Proc Natl Acad Sci USA 101:6905–6910 Rich JN, Eyler CE (2008) Cancer stem cells in brain tumor biology. Cold Spring Harb Symp Quant Biol 73:411–420 Seo JM, Sohn MY, Suh JS, Atala A, Yoo JJ, Shon YH (2011) Cryopreservation of amniotic fluid-derived stem cells using natural cryoprotectants and low concentrations of dimethylsulfoxide. Cryobiology 62:167–173 Simeone DM (2008) Pancreatic cancer stem cells: implications for the treatment of pancreatic cancer. Clin Cancer Res 14:5646–5648 Smith J, Tho LM, Xu N, Gillespie DA (2010) The ATM-Chk2 and ATR-Chk1 pathways in DNA damage signaling and cancer. Adv Cancer Res 108:73–112 Suzuki T, Kiyokawa N, Taguchi T, Sekino T, Katagiri YU, Fujimoto J (2001) CD24 induces apoptosis in human B cells via the glycolipid-enriched membrane domains/rafts-mediated signaling system. J Immunol 166:5567–5577 van der Gun BT, Melchers LJ, Ruiters MH, de Leij LF, McLaughlin PM, Rots MG (2010) EpCAM in carcinogenesis: the good, the bad or the ugly. Carcinogenesis 31:1913–1921 Vasania VS, Prasad P, Gill RK, Mehta A, Viswanathan C, Sarang S, Majumdar AS (2011) Molecular and cellular characterization of expanded and cryopreserved human limbal epithelial stem cells reveal unique immunological properties. Exp Eye Res 92:47–56 Ware CB, Nelson AM, Blau CA (2005) Controlled-rate freezing of human ES cells. Biotechniques 38:879–883 Woods EJ, Benson JD, Agca Y, Critser JK (2004) Fundamental cryobiology of reproductive cells and tissues. Cryobiology 48:146–156 Zhang M, Rosen JM (2006) Stem cells in the etiology and treatment of cancer. Curr Opin Genet Dev 16:60–64 Zocchi E, Usai C, Guida L, Franco L, Bruzzone S, Passalacqua M, De Flora A (1999) Ligand-induced internalization of CD38 results in intracellular Ca2 + mobilization: role of NAD + transport across cell membranes. FASEB J 13:273–283