Molecular epidemiology of Sporothrix schenkii isolates in Malaysia

Medical Mycology - Tập 58 Số 5 - Trang 617-625 - 2020
Nurul Khalisah Kamal Azam1, Gayathri Thevi Selvarajah2, Jacinta Santhanam1, Mohd Fuat Abdul Razak3, Stephanie Jane Ginsapu3, Jasper Elvin James1, Satinee Suetrong4
1Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia
2Faculty of Veterinary Medicine, Universiti Putra Malaysia, Serdang, Malaysia
3Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia
4National Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand Science Park, Pathum Thani, Thailand

Tóm tắt

AbstractSporothrix schenkii is a dimorphic fungus that causes infections in both humans and animals. We report on 25 S. schenkii isolates collected in 2017 from humans and cats clinically diagnosed with sporotrichosis, in Malaysia. These isolates were phenotypically identified as S. schenkii sensu lato and further defined as S. schenckii sensu stricto based on partial calmodulin gene sequence. Isolates from both humans and cats were genotypically identical but displayed phenotypic variation. Phylogenetic analyses based on partial calmodulin sequence showed that the Malaysian isolates clustered with global S. schenkii sensu stricto strains, in particular, of the AFLP type E. This analysis also revealed that partial calmodulin sequence alone was sufficient for classifying global S. schenckii sensu stricto strains into their respective AFLP types, from A to E. The genetically conserved S. schenkii sensu stricto species isolated from humans and cats is suggestive of a clonal strain present in Malaysia. To the best of our knowledge, this is the first report on molecular identification of Sporothrix schenkii strains from human infections in Malaysia. Further studies are required in order to elucidate the clonal nature of Malaysian S. schenkii isolates. Our findings indicate the presence of a predominant S. schenkii genotype in the environment, causing infections in both cats and humans in Malaysia.

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Medical Mycology

Tập 58 Số 5

617-625

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