Định danh phân tử của rêu sừng (Anthocerotophyta) dựa trên vùng DNA diệp lục trnL–trnF

Journal of Plant Research - Tập 116 - Trang 389-398 - 2003
Michael Stech1, Dietmar Quandt2, Wolfgang Frey1
1Institut für Biologie—Systematische Botanik und Pflanzengeographie, Freie Universität Berlin, Berlin, Germany
2Botanisches Institut und Botanischer Garten, Rheinische Friedrich-Wilhelms-Universität Bonn, Bonn, Germany

Tóm tắt

Trong các cây phát sinh chủng loại được tạo ra từ các trình tự intron trnL UAA, rêu sừng (được đại diện bởi chín loài từ các chi Anthoceros, Dendroceros, Megaceros, Notothylas và Phaeoceros) được xác định là một nhóm đơn ngành và tách biệt khỏi các nhánh của rêu, dương xỉ và thực vật có mạch. Một cấu trúc thứ cấp của intron trnL UAA của Anthoceros agrestis được trình bày, thể hiện sự sắp xếp của các vùng stem-loop P1–P9. Các thay đổi cặp bazơ bù (các vị trí đồng tiến hóa) được phát hiện trong các vùng P4/5 và P9 trong các trình tự rêu sừng. Tính đồng đối của vùng biến đổi nhất, P8, không thể phát hiện được nữa do sự tiến hóa phân cách cực kỳ nhanh chóng của đoạn này trong các nhóm thực vật đất chính. Tương tự, sự khác biệt trình tự cao xảy ra trong khoảng cách liên gen trnL–trnF. Ngoài các thay thế đồng sinh trong intron trnL UAA, rêu sừng có đặc điểm là một vùng P6 lớn bao gồm nhiều yếu tố lặp lại. Dữ liệu phân tử do đó hỗ trợ rêu sừng đại diện cho một dòng thực vật đất độc lập (Anthocerotophyta). Tuy nhiên, các mối quan hệ giữa rêu sừng và các nhóm thực vật đất khác vẫn chưa được giải quyết trong các cây intron trnL UAA, và rất không có khả năng rằng rêu là đơn ngành theo cách phân loại truyền thống của chúng, tức là bao gồm rêu sừng, rêu và đẳng hệ.

Từ khóa

#rêu sừng #phân loại học #DNA diệp lục #tiến hóa #thực vật đất

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