Molecular characterization of a microbial consortium involved in methane oxidation coupled to denitrification under micro‐aerobic conditions

Microbial Biotechnology - Tập 7 Số 1 - Trang 64-76 - 2014
Jingjing Liu1,2, Faqian Sun2, Liang Wang2, X. Ju3, Weixiang Wu2, Yingxu Chen2
1Department of Architecture and Resources Engineering Jiangxi University of Science and Technology Nanchang Compus Shuanggang East Road 1180# Nanchang 310013 China
2Institute of Environmental Science and Technology, Zhejiang University, Yuhangtang Road 866#, Hangzhou 310058, China
3Engineering Department Jiangxi Zhongmei Engineering Construction Co., Ltd Nanlian Road 76# Nanchang 330001 China

Tóm tắt

SummaryMethane can be used as an alternative carbon source in biological denitrification because it is nontoxic, widely available and relatively inexpensive. A microbial consortium involved in methane oxidation coupled to denitrification (MOD) was enriched with nitrite and nitrate as electron acceptors under micro‐aerobic conditions. The 16S rRNA gene combined with pmoA phylogeny of methanotrophs and nirK phylogeny of denitrifiers were analysed to reveal the dominant microbial populations and functional microorganisms. Real‐time quantitative polymerase chain reaction results showed high numbers of methanotrophs and denitrifiers in the enriched consortium. The 16S rRNA gene clone library revealed that Methylococcaceae and Methylophilaceae were the dominant populations in the MOD ecosystem. Phylogenetic analyses of pmoA gene clone libraries indicated that all methanotrophs belonged to Methylococcaceae, a type I methanotroph employing the ribulose monophosphate pathway for methane oxidation. Methylotrophic denitrifiers of the Methylophilaceae that can utilize organic intermediates (i.e. formaldehyde, citrate and acetate) released from the methanotrophs played a vital role in aerobic denitrification. This study is the first report to confirm micro‐aerobic denitrification and to make phylogenetic and functional assignments for some members of the microbial assemblages involved in MOD.

Từ khóa


Tài liệu tham khảo

10.1093/nar/25.17.3389

Anthony C., 1982, The Biochemistry of Methylotrophs

10.1007/s002030050527

10.1099/00207713-43-4-735

10.1128/AEM.64.10.3769-3775.1998

10.1007/s002530000337

10.1128/AEM.65.11.5066-5074.1999

10.1007/978-1-4899-2338-7_3

10.1016/S0043-1354(00)00516-9

10.1099/ijs.0.65342-0

10.1111/j.1574-6941.1997.tb00453.x

10.1007/BF00182126

Hanson R.S., 1996, Methanotrophic bacteria, Microbiol Mol Biol Rev, 60, 439

10.1016/B978-0-7506-9188-8.50021-8

10.1111/j.1462-2920.2006.01081.x

10.1111/j.1574-6968.1995.tb07834.x

10.1007/s002530000363

10.1016/B978-1-4832-3211-9.50009-7

10.1111/j.1758-2229.2009.00046.x

10.1128/MMBR.46.1.43-70.1982

10.1007/s00248-007-9320-4

10.1016/j.apsoil.2010.04.006

10.1007/BF00443577

10.1007/s00253-011-3354-8

10.1146/annurev.mi.49.100195.003053

10.1016/j.watres.2007.02.053

10.2166/wst.2008.648

10.1128/AEM.72.2.1346-1354.2006

10.1007/s00248-006-9071-7

10.1111/j.1574-6941.2008.00473.x

Rhee G.Y., 1978, Wastewater denitrification with one‐carbon compounds as energy source, J Water Pollut Control Fed, 50, 2111

10.1007/BF00408378

10.1128/AEM.54.11.2812-2818.1988

10.1007/BF00443743

Saitou N., 1987, The neighbor‐joining method – a new method for reconstructing phylogenetic trees, Mol Biol Evol, 4, 406

10.1016/j.apsoil.2009.11.004

10.1099/ijs.0.65179-0

10.1111/j.1462-2920.2005.00905.x

State Environmental Protection Administration of China, 2002, Methods for Monitor and Analysis of Water and Wastewater

10.1128/AEM.69.6.3152-3157.2003

10.1093/molbev/msm092

10.1016/S0043-1354(96)00228-X

10.1093/nar/25.24.4876

10.1016/j.biortech.2004.08.016

10.1099/00207713-39-3-319

10.1128/AEM.65.11.4887-4897.1999

10.1046/j.1365-2958.2002.02875.x

Thông tin
Thông tin xuất bản

Microbial Biotechnology

Tập 7 Số 1

64-76

Thông tin tác giả