Molecular and immunological characterization of allergens from the entomopathogenic fungus Beauveria bassiana

Clinical and Molecular Allergy - 2006
Greg S. Westwood1, Shih-Wen Huang2, Nemat O. Keyhani1
1Department of Microbiology and Cell Science University of Florida Gainesville USA
2Department of Pediatrics, University of Florida, College of Medicine, USA

Tóm tắt

AbstractBackground

Entomopathogenic fungi such asBeauveria bassianaare considered promising biological control agents for a variety of arthropod pests.Beauveriaspecies, however, have the potential to elicit allergenic reactions in humans, although no specific allergens have been characterized to date.

Methods

Four putative allergens were identified withinB. bassianaexpressed sequence tag (EST) datasets. IgE-reactivity studies were performed using sera from patients displaying mold allergies against recombinantB. bassianaproteins expressed inE. coli.

Results

Full length cDNA and genomic nucleotide sequences of four potentialB. bassianaallergens were isolated. BLASTX search results led to their putative designation as follows; Bb-Eno1, with similarity to fungal enolases; Bb-f2, similar to theAspergillus fumigatusmajor allergen, Asp f2 and to a fibrinogen binding mannoprotein; Bb-Ald, similar to aldehyde dehydrogenases; and Bb-Hex, similar to N-acetyl-hexosaminadases. All four genes were cloned intoE. coliexpression systems and recombinant proteins were produced. Immunoblots ofE. coliextracts probed with pooled as well as individual human sera from patients displaying mould allergies demonstrated IgE reactivity versus recombinant Bb-Eno1 and Bb-Ald.

Conclusion

Four putativeBeauveria bassianaallergens were identified. Recombinant proteins corresponding to two of the four, Bb-Eno1 and Bb-Ald were bound by sera IgEs derived from patients with fungal allergies. These data confirm the potential allergenicity ofB. bassianaby identification of specific human IgE reactive epitopes.

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