Modified chemical method for efficient transformation and diagnosis in Pichia pastoris

Protein Expression and Purification - Tập 174 - Trang 105685 - 2020
Sandeep Kumar1,2, Aruna Mannil3, Sarma Mutturi1,2
1Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India
2Microbiology & Fermentation Technology Department, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, India
3PSG College of Arts and Science, Coimbatore, Tamil Nadu, India

Tài liệu tham khảo

Higgins, 1998 Krainer, 2012, Recombinant protein expression in Pichia pastoris strains with an engineered methanol utilization pathway, Microb. Cell Factories, 1, 22, 10.1186/1475-2859-11-22 Cereghino, 2000, Heterologous protein expression in the methylotrophic yeast Pichia pastoris, FEMS Microbiol. Rev., 24, 45, 10.1111/j.1574-6976.2000.tb00532.x Hartner, 2006, Regulation of methanol utilisation pathway genes in yeasts, Microb. Cell Factories, 5, 39, 10.1186/1475-2859-5-39 Cregg, 2000, Recombinant protein expression in Pichia pastoris, Mol. Biotechnol., 16, 23, 10.1385/MB:16:1:23 Koutz, 1989, Structural comparison of the Pichia pastoris alcohol oxidase genes, Yeast, 53, 167, 10.1002/yea.320050306 Ito, 1983, Transformation of intact yeast cells treated with alkali cations, J. Bacteriol., 153, 163, 10.1128/JB.153.1.163-168.1983 Cregg, 1990 Tolstorukov, 2007, 189 Wu, 2004, High efficiency transformation by electroporation of Pichia pastoris pretreated with lithium acetate and dithiothreitol, Biotechniques, 36, 152, 10.2144/04361DD02 Thompson, 1998, An improved protocol for the preparation of yeast cells for transformation by electroporation, Yeast, 14, 565, 10.1002/(SICI)1097-0061(19980430)14:6<565::AID-YEA251>3.0.CO;2-B Gietz, 2006, Yeast transformation by the LiAc/SS Carrier DNA/PEG method, 107 Tomita, 2002, Isolation of genomic DNA from Pichia pastoris without hydrolases, Biotecnol. Apl., 19, 167 Abad, 2010, Real‐time PCR‐based determination of gene copy numbers in Pichia pastoris, Biotechnol. J., 5, 413, 10.1002/biot.200900233 Mansur, 2005, Multiple gene copy number enhances insulin precursor secretion in the yeast Pichia pastoris, Biotechnol. Lett., 27, 339, 10.1007/s10529-005-1007-7 Romanos, 1998, The generation of multicopy recombinant strains, 55 Sunga, 2008, Post transformational vector amplification in the yeast Pichia pastoris, FEMS Yeast Res., 8, 870, 10.1111/j.1567-1364.2008.00410.x Kumar, 2019, Simplified protocol for faster transformation of (a large number of) Pichia pastoris strains, Yeast, 36, 399, 10.1002/yea.3383 Fujii, 1987, LiCl treatment releases a nickase implicated in genetic transformation of Streptococcus pneumoniae, J. Bacteriol., 169, 4901, 10.1128/JB.169.11.4901-4906.1987 Hamaguchi, 1962, The effect of electrolytes on the stability of the deoxyribonucleate helix, JACS, 84, 1329, 10.1021/ja00867a001 Hatefi, 1969, Solubilization of particulate proteins and nonelectrolytes by chaotropic agents, Proc Natl Acad Sci, 62, 1129, 10.1073/pnas.62.4.1129 Robinson, 1965, The effect of concentrated salt solutions on the activity coefficient of acetyltetraglycine ethyl ester, J. Am. Chem. Soc., 87, 2470, 10.1021/ja01089a029 Cregg, 1989, Functional characterization of the two alcohol oxidase genes from the yeast Pichia pastoris, Mol. Biol. Cell, 9, 1316, 10.1128/MCB.9.3.1316 Zheng, 2014, A simple strategy for the generation of multi‐copy Pichia pastoris with the efficient expression of mannanase, J. Basic Microbiol., 54, 1410, 10.1002/jobm.201400208 Sreekrishna, 1997, Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris, Gene, 190, 55, 10.1016/S0378-1119(96)00672-5 Thill, 1990, Positive and negative effects of multi-copy integrated expression vectors on protein expression in Pichia pastoris, vol. 2, 477 Marx, 2009, Directed gene copy number amplification in Pichia pastoris by vector integration into the ribosomal DNA locus, FEMS Yeast Res., 9, 1260, 10.1111/j.1567-1364.2009.00561.x Soboleski, 2005, Green fluorescent protein is a quantitative reporter of gene expression in individual eukaryotic cells, Faseb. J., 19, 440, 10.1096/fj.04-3180fje Becker, 1991, High‐efficiency transformation of yeast by electroporation, Methods Enzymol., 194, 182, 10.1016/0076-6879(91)94015-5 Lin-Cereghino, 2005, Condensed protocol for competent cell preparation and transformation of the methylotrophic yeast Pichia pastoris, Biotechniques, 38, 44, 10.2144/05381BM04