Tiêu thụ rượu vừa phải làm tăng độ nhạy insulin và biểu hiện gen ADIPOQ ở phụ nữ mãn kinh: một thử nghiệm ngẫu nhiên, giao nhau

Springer Science and Business Media LLC - Tập 51 - Trang 1375-1381 - 2008
M. M. Joosten1,2, J. W. J. Beulens1,2, S. Kersten2, H. F. J. Hendriks1
1Business Unit Biosciences, TNO Quality of Life, Zeist, the Netherlands
2Division of Human Nutrition, Wageningen University, Wageningen, The Netherlands

Tóm tắt

Nghiên cứu nhằm xác định xem việc tiêu thụ rượu vừa phải hàng ngày trong 6 tuần có làm tăng biểu hiện của gen mã hóa adiponectin (ADIPOQ) và nồng độ protein trong huyết tương, cũng như cải thiện độ nhạy insulin ở phụ nữ mãn kinh hay không. Trong một thử nghiệm ngẫu nhiên, mở, giao nhau được thực hiện tại Hà Lan, 36 phụ nữ mãn kinh có sức khỏe bình thường, là người tiêu thụ rượu thường xuyên, đã nhận được 250 ml rượu vang trắng (∼25 g rượu/ngày) hoặc 250 ml nước nho trắng (nhóm đối chứng) hàng ngày trong bữa tối trong 6 tuần. Việc phân bổ điều trị được ngẫu nhiên theo chỉ số khối cơ thể (BMI). Độ nhạy insulin và các mức ADIPOQ mRNA và adiponectin huyết tương được đo vào cuối cả hai giai đoạn. Độ nhạy insulin được ước tính sử dụng mô hình đánh giá trạng thái ổn định tỷ lệ insulin kháng (HOMA-IR). Mức ADIPOQ mRNA trong mô mỡ dưới da được xác định qua RT-PCR. Tất cả các đối tượng đã hoàn thành nghiên cứu. Việc tiêu thụ rượu vang trắng trong 6 tuần đã làm giảm insulin lúc đói (trung bình ± SEM 40.0 ± 3.4 so với 46.5 ± 3.4 pmol/l; p < 0.01) và HOMA-IR (1.42 ± 0.13 so với 1.64 ± 0.13; p = 0.02) so với việc tiêu thụ nước nho trong 6 tuần. Mức ADIPOQ mRNA (1.09 ± 0.15 so với 0.98 ± 0.15; p = 0.04) và nồng độ huyết tương của tổng adiponectin (13.1 ± 0.8 so với 12.0 ± 0.8 μg/ml; p < 0.001) và adiponectin trọng lượng phân tử cao (HMW) (9.9 ± 1.2 so với 8.8 ± 1.2 μg/ml; p = 0.02) đã tăng lên đáng kể sau khi tiêu thụ rượu so với nước nho. Những thay đổi trong mức ADIPOQ mRNA có tương quan với những thay đổi trong mức adiponectin huyết tương tổng thể (ρ = 0.46; p < 0.01). Cả mức triacylglycerol lúc đói (8.2%; p = 0.04) và nồng độ cholesterol LDL (7.8%; p < 0.0001) đều giảm, trong khi cholesterol HDL lại tăng (7.0%; p < 0.0001) sau thời gian tiêu thụ rượu vừa phải kéo dài. Không có tác dụng phụ đáng kể nào được báo cáo. Việc tiêu thụ rượu vừa phải trong 6 tuần cải thiện độ nhạy insulin, mức adiponectin và hồ sơ lipid ở phụ nữ mãn kinh. Hơn nữa, dữ liệu này gợi ý một cơ chế phiên mã dẫn đến sự gia tăng nồng độ adiponectin huyết tương do rượu gây ra. ID ClinicalTrials.gov: NCT00285909 Một phần được tài trợ bởi Quỹ Nghiên cứu Rượu Hà Lan (SAR).

Từ khóa

#rượu vừa phải #độ nhạy insulin #ADIPOQ #phụ nữ mãn kinh #thử nghiệm ngẫu nhiên

Tài liệu tham khảo

Koppes LLJ, Dekker JM, Hendriks HFJ, Bouter LM, Heine RJ (2005) Moderate alcohol consumption lowers the risk of type 2 diabetes: a meta-analysis of prospective observational studies. Diabetes Care 28:719–725 Beulens JWJ, Stolk RP, van der Schouw YT, Grobbee DE, Hendriks HFJ, Bots ML (2005) alcohol consumption and risk of type 2 diabetes among older women. Diabetes Care 28:2933–2938 Hendriks HF (2007) Moderate alcohol consumption and insulin sensitivity: observations and possible mechanisms. Ann Epidemiol 17:S40–S42 Davies MJ, Baer DJ, Judd JT, Brown ED, Campbell WS, Taylor PR (2002) Effects of moderate alcohol intake on fasting insulin and glucose concentrations and insulin sensitivity in postmenopausal women: a randomized controlled trial. JAMA 287:2559–2562 Zilkens RR, Burke V, Watts G, Beilin LJ, Puddey IB (2003) The effect of alcohol intake on insulin sensitivity in men: a randomized controlled trial. Diabetes Care 26:608–612 Sierksma A, Patel H, Ouchi N et al (2004) Effect of moderate alcohol consumption on adiponectin, tumor necrosis factor-α, and insulin sensitivity. Diabetes Care 27:184–189 Beulens JWJ, de Zoete EC, Kok FJ, Schaafsma G, Hendriks HFJ (2008) Effect of moderate alcohol consumption on adipokines and insulin sensitivity in lean and overweight men: a diet intervention study. Eur J Clin Nutr DOI 10.1038/sj.ejcn.1602821 Beulens J, van Loon L, Kok F et al (2007) The effect of moderate alcohol consumption on adiponectin oligomers and muscle oxidative capacity: a human intervention study. Diabetologia 50:1388–1392 Cordain L, Bryan ED, Melby CL, Smith MJ (1997) Influence of moderate daily wine consumption on body weight regulation and metabolism in healthy free-living males. J Am Coll Nutr 16:134–139 Cordain L, Melby CL, Hamamoto AE et al (2000) Influence of moderate chronic wine consumption on insulin sensitivity and other correlates of syndrome X in moderately obese women. Metabolism 49:1473–1478 Beulens JWJ, van Beers RM, Stolk RP, Schaafsma G, Hendriks HFJ (2006) The effect of moderate alcohol consumption on fat distribution and adipocytokines. Obesity Res 14:60–66 Gonzalez-Ortiz M, Pascoe-Gonzalez S, Kam-Ramos AM, Martinez-Abundis E (2005) Effect of tequila on homocysteine, insulin secretion, insulin sensitivity, and metabolic profile in healthy men. J Diabetes Complicat 19:155–159 Chandran M, Phillips SA, Ciaraldi T, Henry RR (2003) Adiponectin: more than just another fat cell hormone? Diabetes Care 26:2442–2450 Tschritter O, Fritsche A, Thamer C et al (2003) Plasma adiponectin concentrations predict insulin sensitivity of both glucose and lipid metabolism. Diabetes 52:239–243 Snijder MB, Heine RJ, Seidell JC et al (2006) Associations of adiponectin levels with incident impaired glucose metabolism and type 2 diabetes in older men and women: the Hoorn Study. Diabetes Care 29:2498–2503 Snehalatha C, Mukesh B, Simon M, Viswanathan V, Haffner SM, Ramachandran A (2003) Plasma adiponectin is an independent predictor of type 2 diabetes in Asian Indians. Diabetes Care 26:3226–3229 Duncan BB, Schmidt MI, Pankow JS et al (2004) Adiponectin and the development of type 2 diabetes: the Atherosclerosis Risk in Communities Study. Diabetes 53:2473–2478 Thamer C, Haap M, Fritsche A, Haering H, Stumvoll M (2004) Relationship between moderate alcohol consumption and adiponectin and insulin sensitivity in a large heterogeneous population. Diabetes Care 27:1240 Englund Ogge L, Brohall G, Behre CJ, Schmidt C, Fagerberg B (2006) Alcohol consumption in relation to metabolic regulation, inflammation, and adiponectin in 64-year-old Caucasian women: a population-based study with a focus on impaired glucose regulation. Diabetes Care 29:908–913 Iwaki M, Matsuda M, Maeda N et al (2003) Induction of adiponectin, a fat-derived antidiabetic and antiatherogenic factor, by nuclear receptors. Diabetes 52:1655–1663 Booyse FM, Aikens ML, Grenett HE (1999) Endothelial cell fibrinolysis: transcriptional regulation of fibrinolytic protein gene expression (t-PA, u-PA, and PAI-1) by low alcohol. Alcohol Clin Exp Res 23:1119–1124 Marx N, Bourcier T, Sukhova GK, Libby P, Plutzky J (1999) PPARgamma activation in human endothelial cells increases plasminogen activator inhibitor type-1 expression: PPARgamma as a potential mediator in vascular disease. Arterioscler Thromb Vasc Biol 19:546–551 Hoiseth G, Bernard JP, Stephanson N et al (2008) Comparison between the urinary alcohol markers EtG, EtS, and GTOL/5-HIAA in a controlled drinking experiment. Alcohol Alcohol 43:187–191 Bottcher M, Beck O, Helander A (2008) Evaluation of a new immunoassay for urinary ethyl glucuronide testing. Alcohol Alcohol 43:46–48 Joosen AM, Bakker AH, Zorenc AH, Kersten S, Schrauwen P, Westerterp KR (2006) PPARgamma activity in subcutaneous abdominal fat tissue and fat mass gain during short-term overfeeding. Int J Obes (Lond) 30:302–307 Arvidsson E, Viguerie N, Andersson I, Verdich C, Langin D, Arner P (2004) Effects of different hypocaloric diets on protein secretion from adipose tissue of obese women. Diabetes 53:1966–1971 Hernandez-Morante JJ, Milagro F, Gabaldon JA, Martinez JA, Zamora S, Garaulet M (2006) Effect of DHEA-sulfate on adiponectin gene expression in adipose tissue from different fat depots in morbidly obese humans. Eur J Endocrinol 155:593–600 Lihn AS, Bruun JM, He G, Pedersen SB, Jensen PF, Richelsen B (2004) Lower expression of adiponectin mRNA in visceral adipose tissue in lean and obese subjects. Mol Cell Endocrinol 219:9–15 Yang WS, Chen MH, Lee WJ et al (2003) Adiponectin mRNA levels in the abdominal adipose depots of nondiabetic women. Int J Obes Relat Metab Disord 27:896–900 Fisher FM, McTernan PG, Valsamakis G et al (2002) Differences in adiponectin protein expression: effect of fat depots and type 2 diabetic status. Horm Metab Res 34:650–654 Motoshima H, Wu X, Sinha MK et al (2002) Differential regulation of adiponectin secretion from cultured human omental and subcutaneous adipocytes: effects of insulin and rosiglitazone. J Clin Endocrinol Metab 87:5662–5667 Rumpler WV, Clevidence BA, Muesing RA, Rhodes DG (1999) Changes in women’s plasma lipid and lipoprotein concentrations due to moderate consumption of alcohol are affected by dietary fat level. J Nutr 129:1713–1717 Sierksma A, Vermunt SH, Lankhuizen IM et al (2004) Effect of moderate alcohol consumption on parameters of reverse cholesterol transport in postmenopausal women. Alcohol Clin Exp Res 28:662–666 Shai I, Wainstein J, Harman-Boehm I et al (2007) Glycemic effects of moderate alcohol intake among patients with type 2 diabetes: a multicenter, randomized, clinical intervention trial. Diabetes Care 30:3011–3016 Bantle AE, Thomas W, Bantle JP (2008) Metabolic effects of alcohol in the form of wine in persons with type 2 diabetes mellitus. Metabolism 57:241–245 DeFronzo RA, Ferrannini E (1991) Insulin resistance. A multifaceted syndrome responsible for NIDDM, obesity, hypertension, dyslipidemia, and atherosclerotic cardiovascular disease. Diabetes Care 14:173–194 Ebrahim S, Lawlor DA, Shlomo YB et al (2008) Alcohol dehydrogenase type 1C (ADH1C) variants, alcohol consumption traits, HDL-cholesterol and risk of coronary heart disease in women and men: British Women’s Heart and Health Study and Caerphilly cohorts. Atherosclerosis 196:871–878 Kern PA, Di Gregorio GB, Lu T, Rassouli N, Ranganathan G (2003) Adiponectin expression from human adipose tissue: relation to obesity, insulin resistance, and tumor necrosis factor-α expression. Diabetes 52:1779–1785 Engeli S, Feldpausch M, Gorzelniak K et al (2003) Association between adiponectin and mediators of inflammation in obese women. Diabetes 52:942–947 Guebre-Egziabher F, Drai J, Fouque D (2007) Adiponectin and chronic kidney disease. J Ren Nutr 17:9–12 Rasouli N, Yao-Borengasser A, Miles LM, Elbein SC, Kern PA (2006) Increased plasma adiponectin in response to pioglitazone does not result from increased gene expression. Am J Physiol Endocrinol Metab 290:E42–E46 Wang Y, Lam KS, Yau MH, Xu A (2008) Post-translational modifications of adiponectin: mechanisms and functional implications. Biochem J 409:623–633 Hara K, Horikoshi M, Yamauchi T et al (2006) Measurement of the high-molecular weight form of adiponectin in plasma is useful for the prediction of insulin resistance and metabolic syndrome. Diabetes Care 29:1357–1362 Bobbert T, Rochlitz H, Wegewitz U et al (2005) Changes of adiponectin oligomer composition by moderate weight reduction. Diabetes 54:2712–2719 Bluher M, Brennan AM, Kelesidis T et al (2007) Total and high-molecular weight adiponectin in relation to metabolic variables at baseline and in response to an exercise treatment program: comparative evaluation of three assays. Diabetes Care 30:280–285 Ouchi N, Walsh K (2007) Adiponectin as an anti-inflammatory factor. Clin Chim Acta 380:24–30 Hung J, McQuillan BM, Thompson PL, Beilby JP (2008) Circulating adiponectin levels associate with inflammatory markers, insulin resistance and metabolic syndrome independent of obesity. Int J Obes (Lond) DOI 10.1038/sj.ijo.0803793 Wellen KE, Hotamisligil GS (2005) Inflammation, stress, and diabetes. J Clin Invest 115:1111–1119 American Institute for Cancer Research (2007) Food, nutrition, physical activity, and the prevention of cancer: a global perspective. World Cancer Research Fund (WCRF) American Institute for Cancer Research (AICR), Washington DC