Mô hình hóa sản xuất bikaverin bởi Fusarium oxysporum CCT7620 trong các nền văn hóa bình lắc

Bioresources and Bioprocessing - Tập 7 Số 1 - 2020
Marcela Colombo dos Santos1, Mayra de Lima Mendonça1, Juliano Lemos Bicas1
1Departamento de Ciência de Alimentos, Universidade Estadual de Campinas, Rua Monteiro Lobato, 80, Campinas, SP, 13083-862, Brazil

Tóm tắt

Tóm tắtBikaverin là một thuốc nhuộm màu đỏ từ nấm với các hoạt tính kháng khuẩn và chống u. Do đó, chất này có thể được sử dụng như một phụ gia thay thế trong ngành thực phẩm và dược phẩm. Mục tiêu của nghiên cứu này là sử dụng phương pháp bề mặt phản hồi để tối ưu hóa các điều kiện lên men và tối đa hóa sản xuất bikaverin trong các bình lắc. Các biến số được điều tra bao gồm tốc độ khuấy (71–289 vòng/phút), nhiệt độ (21–35 °C) và nồng độ chất nền (gạo) trong môi trường văn hóa (16.4–83.6 g/L). Tốc độ khuấy có tác động tích cực đến sản xuất thuốc nhuộm màu đỏ, trong khi nồng độ chất nền và nhiệt độ có tác động ngược lại. Sản xuất bikaverin tối đa được dự đoán sẽ xảy ra ở tốc độ 289 vòng/phút, nhiệt độ 24.3 °C và nồng độ gạo 16.4 g/L. Việc xác nhận thực nghiệm với tốc độ 289 vòng/phút, 28 °C và nồng độ gạo 20 g/L cho kết quả cao hơn 6.2% so với dự đoán của mô hình. Nghiên cứu này có tầm quan trọng trong việc xác định các điều kiện tốt nhất cho sản xuất bikaverin.

Từ khóa


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Bioresources and Bioprocessing

Tập 7 Số 1

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