Mitogenic Activity of Sulfated Chitosan and Cellulose Derivatives is Related to Protection of FGF‐2 from Proteolytic Cleavage

Macromolecular Bioscience - Tập 12 Số 6 - Trang 740-750 - 2012
Annett Weltrowski1, Sidalina Almeida, Dieter Peschel, Kai Zhang, Steffen Fischer, Thomas Groth
1Department of Pharmaceutics and Biopharmaceutics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Heinrich-Damerow-Strasse 4, 06120 Halle-Saale, Germany.

Tóm tắt

AbstractNovel chitosan (CHS) and cellulose sulfates (CSs) are studied regarding their mitogenic activity and their protective effect against proteolytic digestion of FGF‐2. An intermediate degree of sulfation (DSS) and lower concentration of CHS have superior effect on 3T3 cell growth while the mitogenic activity of CS increases with DSS and concentration. Experiments with trypsin as model proteinase show that protection of FGF‐2 from proteolytic digestion depends on DSS and the concentration of derivatives in the same manner as cell growth. Studies on stability of FGF‐2 added to cultures of 3T3 cells show that the FGF‐2 concentration remains higher in the presence of derivatives. Results indicate that the mitogenic activity of CHS and CS is due to protection of FGF‐2 from proteolytic cleavage.magnified image

Từ khóa


Tài liệu tham khảo

10.1016/j.jbiomech.2009.09.009

10.1677/joe.0.1720221

10.1152/physiol.00045.2009

10.1146/annurev.bi.58.070189.003043

Baird A., 1991, Ann. NY Acad. Sci, 1

10.1016/0092-8674(94)90520-7

10.1083/jcb.109.4.1877

10.1016/0968-0004(86)90271-9

10.1016/0955-2235(89)90012-4

10.1002/1521-3757(20020201)114:3<426::AID-ANGE426>3.0.CO;2-Q

10.1016/S0092-8674(03)00715-3

10.1083/jcb.200212083

10.1091/mbc.10.2.313

10.1021/bi011000u

10.1002/jcp.1041280317

10.1111/j.1432-1033.1993.tb17895.x

10.1002/jcp.1041380129

10.1016/S0142-9612(97)00050-1

10.1016/S0008-6215(02)00163-5

10.1002/masy.200850216

10.1002/masy.200950604

10.1016/j.polymer.2010.08.034

10.1016/j.carbpol.2010.07.021

10.1016/j.actbio.2009.12.032

10.1016/B978-0-08-020367-6.50007-6

10.1080/02648725.1996.10647935

10.1007/b136820

10.1016/j.ijfoodmicro.2010.09.012

10.1016/j.carbpol.2005.05.022

10.1016/j.carbpol.2005.04.007

10.1016/S0008-6215(97)00117-1

10.1002/3527601937

10.1016/j.biomaterials.2008.12.016

10.1016/j.actbio.2011.08.012

10.1016/S0169-409X(02)00029-7

10.1517/13543776.14.6.859

Stryer L., 1996, Biochemie, 259

10.1007/128_068

10.1016/j.semnephrol.2009.03.009

10.1021/bc900208t

10.1002/mabi.200900107

10.1002/mabi.201000086

10.1002/mabi.200400026