Migration of intervertebral disc cells into dense collagen scaffolds intended for functional replacement

Springer Science and Business Media LLC - Tập 23 - Trang 813-821 - 2012
Johannes L. Bron1, Harriet W. Mulder1, Lucienne A. Vonk2, Bherouz Zandieh Doulabi1, M. J. Oudhoff3, Theodoor H. Smit1,4
1Department of Orthopaedic Surgery, VU University Medical Center, Amsterdam, The Netherlands
2Department of Orthopaedic Research, University Medical Centre Utrecht, Utrecht, The Netherlands
3Department of Oral Biochemistry, Academic Centre for Dentistry, University of Amsterdam, Amsterdam, The Netherlands
4Research Institute MOVE, VU University Amsterdam and Skeletal Tissue Engineering Group Amsterdam (STEGA), Amsterdam, The Netherlands

Tóm tắt

Invasion of cells from surrounding tissues is a crucial step for regeneration when using a-cellular scaffolds as a replacement of the nucleus pulposus (NP). The aim of current study was to assess whether NP and surrounding annulus fibrosus (AF) cells are capable of migrating into dense collagen scaffolds. We seeded freshly harvested caprine NP and AF cells onto scaffolds consisting of 1.5 and 3.0% type I collagen matrices, prepared by plastic compression, to assess cell invasion. The migration distance appeared both time and density dependent and was higher for NP (25%) compared to AF (10%) cells after 4 weeks. Migration distance was not enhanced by Hst-2, a peptide derived from saliva known to enhance fibroblast migration, and this was confirmed in a scratch assay. In conclusion, we revealed invasion of cells into dense collagen scaffolds and therewith encouraging first steps towards the use of a-cellular scaffolds for NP replacement.

Tài liệu tham khảo

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