Migration of human vascular smooth muscle cells involves serum-dependent repeated cytosolic calcium transients

Journal of Cell Science - Tập 113 Số 4 - Trang 653-662 - 2000
Arnaud Scherberich1, Manuel Campos‐Toimil1, Philippe Rondé1, Kenneth Takeda1, Alain Beretz1
1Université Louis Pasteur de Strasbourg Pharmacologie et Physico-Chimie des Interactions Cellulaires et Moléculaires, UMR CNRS 7034, Faculté de Pharmacie , , Illkirch, France

Tóm tắt

ABSTRACT Migration of vascular smooth muscle cells (VSMC) is a key event in the formation of neointima during atherosclerosis. Fura-2 loaded VSMCs were used to investigate calcium homeostasis during cell migration. Multiple spontaneous transient increases in cytosolic free calcium [Ca2+]iwere observed in single human VSMCs migrating on type I collagen. Such [Ca2+]itransients were dependent on the presence of serum or PDGF-BB. Removal of serum, or loading cells with BAPTA, abolished the transients and decreased cell migration speed. The transients were not affected by disruption of cell polarization by dihydrocytochalasin B. Adhesion was used to investigate the specific role of cell-substrate interactions in the generation of transients. Transients are seen in VSMCs adhering either on collagen or on poly-L-lysine, suggesting that generation of transients is not strictly dependent on integrins. Buffering [Ca2+]iwith BAPTA led to accumulation of β1 integrins at the cellular tail, and to increased release of integrin on the extracellular matrix. These results demonstrate a role for [Ca2+]itransients in the rapid, serum-dependent migration of VSMCs. These [Ca2+]itransients are present in migrating VSMCs only when two simultaneous events occur: (1) substrate independent spreading and (2) stimulation of cells by serum components such as PDGF-BB.

Từ khóa


Tài liệu tham khảo

Aplin, 1998, Signal transduction and signal modulation by cell adhesion receptors: the role of integrins, cadherins, immunoglobulin-cell adhesion molecules, and selectins, Pharmacol. Rev, 50, 197

Cunningham, 1991, Enhanced motility in NIH 3T3 fibroblasts that overexpress gelsolin, Science, 251, 1233, 10.1126/science.1848726

Gomez, 1999, In vivo regulation of axon extension and pathfinding by growth-cone calcium transients, Nature, 397, 350, 10.1038/16927

Gotwals, 1996, The α1β1 integrin is expressed during neointima formation in rat arteries and mediates collagen matrix reorganization, J. Clin. Invest, 97, 2469, 10.1172/JCI118693

Grynkiewicz, 1985, A new generation of Ca2+ indicators with greatly improved fluorescence properties, J. Biol. Chem, 260, 3440, 10.1016/S0021-9258(19)83641-4

Harris, 1990, Calmodulin and calcium-dependent protease I coordinately regulate the interaction of fodrin with actin, Proc. Natl. Acad. Sci. USA, 87, 3009, 10.1073/pnas.87.8.3009

Hendey, 1992, Inhibition of neutrophil chemokinesis on vitronectin by inhibitors of calcineurin, Science, 258, 296, 10.1126/science.1384129

Hughes, 1998, Integrin affinity modulation, Trends Cell Biol, 8, 359, 10.1016/S0962-8924(98)01339-7

Huttenlocher, 1996, Modulation of cell migration by integrin-mediated cytoskeletal linkages and ligand-binding affinity, J. Cell Biol, 134, 1551, 10.1083/jcb.134.6.1551

Jaconi, 1991, Multiple elevations of cytosolic-free Ca2+ in human neutrophils: initiation by adherence receptors of the integrin family, J. Cell Biol, 112, 1249, 10.1083/jcb.112.6.1249

Komuro, 1996, Intracellular Ca2+ fluctuations modulate the rate of neuronal migration, Neuron, 17, 275, 10.1016/S0896-6273(00)80159-2

Lauffenburger, 1996, Cell migration: a physically integrated molecular process, Cell, 84, 359, 10.1016/S0092-8674(00)81280-5

Lawson, 1995, Ca(2+)- and calcineurin-dependent recycling of an integrin to the front of migrating neutrophils, Nature, 377, 75, 10.1038/377075a0

Leavesley, 1993, Integrin β1 and β3-mediated endothelial cell migration is triggered through distinct signaling mechanisms, J. Cell Biol, 121, 163, 10.1083/jcb.121.1.163

Lee, 1993, How do cells move along surfaces?, Trends Cell Biol, 3, 366, 10.1016/0962-8924(93)90084-E

Lee, 1999, Regulation of cell movement is mediated by stretch-activated calcium channels, Nature, 400, 382, 10.1038/22578

Lynch, 1994, A pertussis toxin-insensitive calcium influx mediated by neuropeptide Y2 receptors in a human neuroblastoma cell line, J. Biol. Chem, 269, 8226, 10.1016/S0021-9258(17)37183-1

Mandeville, 1995, Intracellular calcium levels correlate with speed and persistent forward motion in migrating neutrophils, Biophys. J, 68, 1207, 10.1016/S0006-3495(95)80336-X

Marks, 1990, Transient increases in cytosolic free calcium appear to be required for the migration of adherent human neutrophils, J. Cell Biol, 110, 43, 10.1083/jcb.110.1.43

Marks, 1991, Attachment to fibronectin or vitronectin makes human neutrophil migration sensitive to alterations in cytosolic free calcium concentration, J. Cell Biol, 112, 149, 10.1083/jcb.112.1.149

Paladichuk, 1998, Cells on the move, The Scientist, 12, 19

Palecek, 1996, Integrin dynamics on the tail region of migrating fibroblasts, J. Cell Sci, 109, 941, 10.1242/jcs.109.5.941

Pettit, 1996, Localised and global cytosolic Ca2+ changes in neutrophils during engagement of CD11b/CD18 integrin visualised using confocal laser scanning reconstruction, J. Cell Sci, 109, 1689, 10.1242/jcs.109.7.1689

Pickering, 1997, Fibroblasts growth factor-2 potentiates vascular smooth muscle cell migration to platelet-derived growth factor. Upregulation of α2β1 integrin and disassembly of actin filaments, Circ. Res, 80, 627, 10.1161/01.RES.80.5.627

Poole, 1995, Regulation of cytosolic calcium by collagen in single human platelets, Br. J. Pharmacol, 115, 101, 10.1111/j.1476-5381.1995.tb16326.x

Ross, 1971, Smooth muscle cells. II. Growth of smooth muscle in culture and formation of elastic fibers, J. Cell Biol, 50, 172, 10.1083/jcb.50.1.172

Ross, 1993, The pathogenesis of atherosclerosis: a perspective for the 1990s, Nature, 362, 801, 10.1038/362801a0

Scherberich, 1998, Tetraspanin CD9 is associated with Very Late-Acting integrins in human vascular smooth muscle cells and modulates collagen matrix reorganization, Arterioscl. Thromb. Vasc. Biol, 18, 1691, 10.1161/01.ATV.18.11.1691

Schmidt, 1995, Modulation of cell migration speed via genetic manipulation of integrin/cytoskeleton linkage, J. Cell. Eng, 1, 3

Sjaastad, 1994, Feedback regulation of cell-substratum adhesion by integrin-mediated intracellular Ca2+ signaling, Proc. Natl. Acad. Sci. USA, 91, 8214, 10.1073/pnas.91.17.8214

Sjaastad, 1997, Integrin-mediated calcium signaling and regulation of cell adhesion by intracellular calcium, BioEssays, 19, 47, 10.1002/bies.950190109

Skinner, 1994, Dynamic expression of alpha 1 beta 1 and alpha 2 beta 1 integrin receptors by human vascular smooth muscle cells. Alpha 2 beta 1 integrin is required for chemotaxis across type I collagen-coated membranes, Am. J. Pathol, 145, 1070

Stossel, 1993, On the crawling of animal cells, Science, 260, 1086, 10.1126/science.8493552

Wojnowski, 1994, Cell transformation induces a cytoplasmic Ca2+ oscillator in Madin-Darby canine kidney cells, Pflügers Arch, 426, 89, 10.1007/BF00374675