Microstructural and in-vitro characteristics of functional calcium silicate topcoat on hydroxyapatite coating for bio-implant applications

Progress in Biomaterials - Tập 11 - Trang 95-108 - 2022
Jarnail Singh1, Sukhpal Singh Chatha1, Hazoor Singh1
1Yadavindra College of Engineering, Punjabi University Guru Kashi Campus, Talwandi Sabo, India

Tóm tắt

The delayed tissue–implant interactions in metallic implants coated with hydroxyapatite (HA) paved the way for the development of alternative bioactive coatings. In this study, bi-layered functional gradient (HA-CS) coating was formulated by the atmospheric plasma spray (APS) process on Ti6Al4V alloy. The HA layer was applied at the metal interface to ensure long-term stability, while the calcium silicate (CS) outer layer was applied to achieve fast tissue–implant interactions. Moreover, single-layered HA and CS coating were also formulated for comparative analysis. The phase compositions, coating microstructure, chemical properties, microhardness, porosity, surface roughness, and in-vitro bioactivity were investigated. The CS top layer showed high porosity and surface roughness with respect to the inner HA layer, which constitutes an optimum microstructure to promote bioactivity. The microhardness of the outer CS layer of HA-CS was 520.3 ± 80.8 HV, while the corresponding value for the inner HA layer was 291.7 ± 45.7 HV. HA-CS and CS coatings demonstrated higher in-vitro bioactivity compared to HA coating. On the contrary, HA coating (3.76 mpy) displayed better corrosion resistance than the HA-CS (4.17 mpy) and CS coatings (4.34 mpy). The in-vitro results indicated that the HA-CS coating could promote the healthy development of osteoblast-like MG-63.

Tài liệu tham khảo

Amaravathy P, Sowndarya S, Sathyanarayanan S, Rajendran N (2014) Novel sol gel coating of Nb2O5 on magnesium alloy for biomedical applications. Surf Coat Technol 244:131–141. https://doi.org/10.1016/j.surfcoat.2014.01.050 Atif M, Afzal F, Kesarwani P et al (2012) Functionally graded hydroxyapatite-alumina-zirconia biocomposite : Synergy of toughness and biocompatibility. Mater Sci Eng C 32:1164–1173. https://doi.org/10.1016/j.msec.2012.03.003 Baheiraei N, Azami M, Hosseinkhani H (2015) Investigation of magnesium incorporation within gelatin/calcium phosphate nanocomposite scaffold for bone tissue engineering. Int J Appl Cera Tech 12:245–253. https://doi.org/10.1111/ijac.12189 Balani K, Anderson R, Laha T et al (2007) Plasma-sprayed carbon nanotube reinforced hydroxyapatite coatings and their interaction with human osteoblasts in vitro. Biomaterials 28:618–624. https://doi.org/10.1016/j.biomaterials.2006.09.013 Bansal P, Singh G, Sidhu HS (2020) Investigation of corrosion behavior and surface properties of plasma sprayed HA/Sr reinforced coatings on CoCr alloys. Mat Chem Phys 253:123330. https://doi.org/10.1016/j.matchemphys.2020.123330 Beheri HH, Mohamed KR, El-bassyouni GT (2013) Mechanical and microstructure of reinforced hydroxyapatite/calcium silicate nano-composites materials. J Mat Des 44:461–468. https://doi.org/10.1016/j.matdes.2012.08.020 Bolelli G, Cannillo V, Gadow R et al (2009) Microstructural and in vitro characterisation of high-velocity suspension flame sprayed (HVSFS) bioactive glass coatings. J Eur Ceram Soc 29:2249–2257. https://doi.org/10.1016/j.jeurceramsoc.2009.01.032 Cattini A, Bellucci D et al (2013) Suspension plasma sprayed bioactive glass coatings: effects of processing on microstructure, mechanical properties and in-vitro behaviour. Surf Coat Technol 220:52–59. https://doi.org/10.1016/j.surfcoat.2012.10.076 Cattini A, Bellucci D, Sola A et al (2014a) Functional bioactive glass topcoats on hydroxyapatite coatings: analysis of microstructure and in-vitro bioactivity. Surf Coat Technol 240:110–117. https://doi.org/10.1016/j.surfcoat.2013.12.023 Cattini A, Bellucci D, Sola A et al (2014b) Functional bioactive glass topcoats on hydroxyapatite coatings: analysis of microstructure and in-vitro bioactivity. Surf Coat Technol 240:110–117. https://doi.org/10.1016/j.surfcoat.2013.12.023 Cattini A, Bellucci D, Sola A et al (2014c) Microstructural design of functionally graded coatings composed of suspension plasma sprayed hydroxyapatite and bioactive glass. J Biomed Mater Res Part B Appl Biomater 102:551–560. https://doi.org/10.1002/jbm.b.33034 Dehghanian C, Aboudzadeh N, Shokrgozar MA (2018) Characterization of silicon-substituted nano hydroxyapatite coating on magnesium alloy for biomaterial application. Mater Chem Phys 203:27–33. https://doi.org/10.1016/j.matchemphys.2017.08.020 Deligianni DD, Katsala ND, Koutsoukos PG, Missirlis YF (2000) Effect of surface roughness of hydroxyapatite on human bone marrow cell adhesion, proliferation, differentiation and detachment strength. Biomaterials 22:87–96. https://doi.org/10.1016/S0142-9612(00)00174-5 Gkomoza P, Vardavoulias M, Pantelis DI, Sarafoglou C (2019) Comparative study of structure and properties of thermal spray coatings using conventional and nanostructured hydroxyapatite powder, for applications in medical implants. Surf Coat Technol 357:748–758. https://doi.org/10.1016/j.surfcoat.2018.10.044 Hu H, Qiao Y, Meng F et al (2013) Enhanced apatite-forming ability and cytocompatibility of porous and nanostructured TiO2/CaSiO3 coating on titanium. Colloids Surf B Biointerfaces 101:83–90. https://doi.org/10.1016/j.colsurfb.2012.06.021 Huang Y, Han S, Pang X et al (2013) Electrodeposition of porous hydroxyapatite/calcium silicate composite coating on titanium for biomedical applications. Appl Surf Sci 271:299–302. https://doi.org/10.1016/j.apsusc.2013.01.187 Huang C-F, Chiang H-J, Lin H-J et al (2014) Comparison of cell response and surface characteristics on titanium implant with SLA and SLAffinity functionalization. J Electrochem Soc 161:G15–G20. https://doi.org/10.1149/2.084403jes Huang Y, Zhang H, Qiao H et al (2015) Anticorrosive effects and in vitro cytocompatibility of calcium silicate/zinc-doped hydroxyapatite composite coatings on titanium. Appl Surf Sci 357:1776–1784. https://doi.org/10.1016/j.apsusc.2015.10.034 Jemat A, Ghazali MJ, Razali M et al (2017) Effects of TiO2 on microstructural, mechanical properties and in-vitro bioactivity of plasma sprayed yttria stabilised zirconia coatings for dental application. Ceram Int 44:4271–4281. https://doi.org/10.1016/j.ceramint.2017.12.008 Kang AS (2020) Wear performance of hydroxyapatite coatings deposited on AISI 304L using detonation gun spray. Mater Today: Proc. https://doi.org/10.1016/j.matpr.2020.01.378 Khor KA, Gu YW, Pan D, Cheang P (2004) Microstructure and mechanical properties of plasma sprayed HA/YSZ/Ti–6Al–4V composite coatings. Biomaterials 25:4009–4017. https://doi.org/10.1016/j.biomaterials.2003.10.089 Kumari R, Dutta J (2017a) Materials characterization microstructure and surface mechanical properties of plasma spray deposited and post spray heat treated hydroxyapatite (HA) based composite coating on titanium alloy (Ti-6Al-4V) substrate. Mater Charact 131:12–20. https://doi.org/10.1016/j.matchar.2017.06.011 Kumari R, Dutta J (2017b) Studies on corrosion resistance and bio-activity of plasma spray deposited hydroxylapatite (HA) based TiO2 and ZrO2 dispersed composite coatings on titanium alloy ( Ti-6Al-4V ) and the same after post spray heat treatment. Appl Surf Sci 420:935–943. https://doi.org/10.1016/j.apsusc.2017.05.208 Li P, Xie C, Liu J et al (2014) Fabrication of nano-structured calcium silicate coatings with enhanced stability, bioactivity and osteogenic and angiogenic activitySurface and Coatings Technology Titanium dioxide nanotubes/calcium silicate composite coatings prepared by alternative loop. Surf Coat Technol 258:624–630. https://doi.org/10.1016/j.surfcoat.2014.08.026 Liu X, Ding C (2001) Phase compositions and microstructure of plasma sprayed wollastonite coating. Surf Coat Technol. https://doi.org/10.1016/S0257-8972(01)01169-0 Liu X, Ding C (2002) Characterization of plasma sprayed wollastonite powder and coatings. Surf Coat Technol 153:173–177. https://doi.org/10.1016/S0257-8972(01)01666-8 Liu X, Ding C, Wang Z (2001) Apatite formed on the surface of plasma-sprayed wollastonite coating immersed in simulated body fluid. Biomaterials 22:2007–2012. https://doi.org/10.1016/S0142-9612(00)00386-0 Liu X, Ding C, Chu PK (2004) Mechanism of apatite formation on wollastonite coatings in simulated body fluids. Biomaterails 25:1755–1761. https://doi.org/10.1016/j.biomaterials.2003.08.024 Lu Y, Li M, Li S et al (2004) Plasma-sprayed hydroxyapatite + titania composite bond coat for hydroxyapatite coating on titanium substrate. Biomaterials 25:4393–4403. https://doi.org/10.1016/j.biomaterials.2003.10.092 Mittal M, Nath SK, Prakash S (2013) Improvement in mechanical properties of plasma sprayed hydroxyapatite coatings by Al2O3 reinforcement. Mater Sci Eng C 33:2838–2845. https://doi.org/10.1016/j.msec.2013.03.005 Mohajernia S, Pour-Ali S, Hejazi S et al (2018) Hydroxyapatite coating containing multi-walled carbon nanotubes on AZ31 magnesium: mechanical-electrochemical degradation in a physiological environment. Ceram Int 44:8297–8305. https://doi.org/10.1016/j.ceramint.2018.02.015 Ou KL, Hosseinkhani H (2014) Development of 3D in vitro technology for medical applications. Int J Mol Sci 15:17938–17962 Ou S-F, Chen C-S, Hosseinkhani H (2013) Surface properties of nano-structural silicon-doped carbon films for biomedical applications Ou KL, Chu JS, Hosseinkhani H et al (2014) Biomedical nanostructured coating for minimally invasive surgery devices applications: characterization, cell cytotoxicity evaluation and an animal study in rat. Surg Endosc 28:2174–2188. https://doi.org/10.1007/s00464-014-3450-9 Pawlowski L, Bigan M, Jaworski R et al (2010) Phase evolution of hydroxapatite coatings suspension plasma sprayed using variable parameters in simulated body fluid. Surf Coat Technol 204:1236–1246. https://doi.org/10.1016/j.surfcoat.2009.10.022 Pham DQ, Berndt CC, Gbureck U et al (2019) Mechanical and chemical properties of Baghdadite coatings manufactured by atmospheric plasma spraying. Surf Coat Technol 378:124945. https://doi.org/10.1016/j.surfcoat.2019.124945 Rechendorff K, Hovgaard MB, Foss M et al (2006) Enhancement of protein adsorption induced by surface roughness. Langmuir 22:10885–10888. https://doi.org/10.1021/la0621923 Singh G, Singh S, Prakash S (2011) Surface characterization of plasma sprayed pure and reinforced hydroxyapatite coating on Ti6Al4V alloy. Surf Coat Technol 205:4814–4820. https://doi.org/10.1016/j.surfcoat.2011.04.064 Singh A, Singh G, Chawla V (2018a) Influence of post coating heat treatment on microstructural, mechanical and electrochemical corrosion behaviour of vacuum plasma sprayed reinforced hydroxyapatite coatings. J Mech Behav Biomed Mater 85:20–36. https://doi.org/10.1016/j.jmbbm.2018.05.030 Singh A, Singh G, Chawla V (2018b) Characterization and mechanical behavior of reinforced hydroxyapatite coatings deposited by vacuum plasma spray on SS-316L alloy. J Mech Behav Biomed Mater 79:273–282. https://doi.org/10.1016/j.jmbbm.2018.01.005 Singh B, Singh G, Singh B (2018c) Analysis of corrosion behavior and surface properties of plasma-sprayed HA/Ta coating on CoCr alloy. J Therm Spray Technol. https://doi.org/10.1007/s11666-018-0786-z Singh J, Singh S, Singh H (2018d) Characterization and corrosion behavior of functional gradient hydroxyapatite coating. J Therm Spray Technol. https://doi.org/10.1007/s11666-018-0802-3 Singh B, Singh G, Singh B (2019a) In vitro investigation of Nb-Ta alloy coating deposited on CoCr alloy for biomedical implants. Surf Coat Technol 377:124932. https://doi.org/10.1016/j.surfcoat.2019.124932 Singh B, Singh G, Singh B, Bhatia N (2019b) In-vitro assessment of HA-Nb coating on Mg alloy ZK60 for biomedical applications. Mater Chem Phys 231:138–149. https://doi.org/10.1016/j.matchemphys.2019.04.037 Singh A, Singh G, Chawla V (2020a) Materials Today: proceedings in-vitro performance of reinforced hydroxyapatite coatings deposited using vacuum plasma spray technique on Ti-6Al-4V. Mater Today Proc. https://doi.org/10.1016/j.matpr.2019.12.363 Singh S, Pandey KK, Islam A, Keshri AK (2020b) Corrosion behaviour of plasma sprayed graphene nanoplatelets reinforced hydroxyapatite composite coatings in simulated body fluid. Ceram Int. https://doi.org/10.1016/j.ceramint.2020.02.139 Singh S, Prakash C, Singh H (2020c) Deposition of HA-TiO2 by plasma spray on β-phase Ti-35Nb-7Ta-5Zr alloy for hip stem: characterization, mechanical properties, corrosion, and in-vitro bioactivity. Surf Coat Technol 398:126072. https://doi.org/10.1016/j.surfcoat.2020.126072 Singh J, Chatha SS, Singh H (2021a) Characterization and corrosion behavior of plasma sprayed calcium silicate reinforced hydroxyapatite composite coatings for medical implant applications. Ceram Int 47:782–792. https://doi.org/10.1016/j.ceramint.2020.08.189 Singh J, Chatha SS, Singh H (2021b) Synthesis and characterization of plasma sprayed functional gradient bioceramic coating for medical implant applications. Ceram Int 47:9143–9155. https://doi.org/10.1016/j.ceramint.2020.12.039 Wang X, Zhou Y, Xia L et al (2014) Fabrication of nano-structured calcium silicate coatings with enhanced stability, bioactivity and osteogenic and angiogenic activity. Colloids Surf B Biointerfaces. https://doi.org/10.1016/j.colsurfb.2014.11.044 Wang H, Zheng Y, Jiang C et al (2017) In vitro corrosion behavior and cytocompatibility of pure Fe implanted with Ta. Surf Coat Technol 320:201–205. https://doi.org/10.1016/j.surfcoat.2017.01.051 Xue W, Liu X, Zheng X, Ding C (2005) In vivo evaluation of plasma-sprayed wollastonite coating. Biomaterials 26:3455–3460. https://doi.org/10.1016/j.biomaterials.2004.09.027 Yang Y, Wang Y, Tian W et al (2015) Influence of composite powders’ microstructure on the microstructure and properties of Al2O3-TiO2 coatings fabricated by plasma spraying. Mater Des 65:814–822. https://doi.org/10.1016/j.matdes.2014.09.078 Yugeswaran S, Yoganand CP, Kobayashi A, Paraskevopoulos KM (2012) Mechanical properties, electrochemical corrosion and in-vitro bioactivity of yttria stabilized zirconia reinforced hydroxyapatite coatings prepared by gas tunnel type plasma spraying. J Mech Behav Biomed Mater 9:22–33. https://doi.org/10.1016/j.jmbbm.2011.11.002 Yusuf M, Bakar A, Muhamad N, Rafi M (2019) Incorporation of wollastonite bioactive ceramic with titanium for medical applications: an overview. Mater Sci Eng C 97:884–895. https://doi.org/10.1016/j.msec.2018.12.056