Microsatellite multiplex assay for the analysis of Atlantic sturgeon populations

Journal of Applied Genetics - Tập 55 - Trang 505-510 - 2014
H. Panagiotopoulou1,2, D. Popovic3, K. Zalewska4, P. Weglenski1,3, A. Stankovic1,4,5
1Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland
2Institute of Genetics and Biotechnology, University of Warsaw, Warsaw, Poland
3Centre of New Technologies (CeNT), University of Warsaw, Warsaw, Poland
4Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Warsaw, Poland
5The Antiquity of Southeastern Europe Research Center, University of Warsaw, Warsaw, Poland

Tóm tắt

We have developed a multiplex assay covering 16 microsatellite loci, amplified in four polymerase chain reaction (PCR) assays, and loaded on the ABI DNA Analyzer in two separate panels. The assay was tested on 603 individuals originating from wild populations and hatchery stocks of Atlantic sturgeon. The assay was also tested on 12 individuals of European sturgeon and appeared to be almost equally useful. The multiplex assay designed in this study can be successfully applied in studies requiring high genetic resolution, such as relatedness analysis, selective breeding programs, and stock identification of Atlantic sturgeon.

Tài liệu tham khảo

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