Microneutralization tests for serological typing and subtyping of foot-and-mouth disease virus strains

Cambridge University Press (CUP) - Tập 81 Số 1 - Trang 107-123 - 1978
Mark M. Rweyemamu1, J. C. Booth1, M. Head1, T. W. F. Pay1
1The Wellcome Foundation Limited, Wellcome Foot-and-Mouth Disease Vaccine Leboratory, Pirbright, Woking, Surrey 0U24 ONQ, U.K.

Tóm tắt

SUMMARYA microneutralization test for serotyping of FMD viruses is described. It is based on earlier observations by Booth, Rweyemamu & Pay (1978) that dose-response relationships in quantal microneutralizations often deviated from linearity. The typing test described therefore utilizes undiluted virus preparations. In about 90% of samples a positive typing was obtained in contrast with about 50% for the complement fixation test. The test was also found to be susceptible to minimal quantities of heterotypic viral contamination.For strain differentiation the microneutralization test was carried out as a checkerboard test. When compared with the complement fixation test it was found to be more specific. The necessity to utilize virus-neutralization test systems for comparing (FMD) virus strains particularly for the purpose of vaccine selection is emphasized. The two dimensional microneutralization test has been applied to a study of comparing FMDV vaccine strains for Europe, South America, the Middle East and East Africa.

Từ khóa


Tài liệu tham khảo

Pay, 1972, Some observations on the potency testing of foot-and-mouth disease vaccine, Bulletin Office International des Epizooties, 79, 1175

10.1007/BF01242147

10.1007/BF01250230

Brooksby J. B. (1968). Variants and immunity: definitions for serological investigations. International Symposium on Foot-and Mouth Disease. Variants and Immunity. Lyon, 1967. Symposium Series. Immunobiological Standardization 8, 1–10.

Rweyemamu M. M. , Pay T. W. F. & Parker M. J. (1977 c). Serological differentiation of foot-and-mouth disease virus strains in relation to selection of suitable vaccine viruses. International Symposium on Foot-and-Mouth Disease. Lyon, 1976. Developments in Biological Standardization 35, 205–14.

10.1099/0022-1317-7-2-115

Rweyemamu, 1975, Neutralization kinetics studies with type SAT 2 foot-and-mouth disease virus strains, Proceedings of the 20th World Veterinary Congress, 3, 2523

10.1017/S002217240002427X

Martin, 1961, The tissue culture colour test for assaying the virus and neutralizing antibody of foot-and-mouth disease virus and its application to the measurement of immunity in cattle, Research in Veterinary Science, 2, 53, 10.1016/S0034-5288(18)34978-6

Cottral, 1972, Proceedings of the 76th Annual Meeting of U.S. Animal Health Association, Florida, 194

Sugimura, 1976, Typing of foot-and-mouth disease virus by fluorescent antibody technique, National Institute of Animal Health Quarterly, 16, 152

10.1017/S0022172400053377

10.1016/S0065-2776(08)60733-6

Pereira, 1977, International Symposium on Foot-and-Mouth Disease, Developments in Biological Standardization, 35, 167

Brooksby, 1952, The technique of complement fixation in foot-and-mouth disease research, Agricultural Research Council Report Series

10.1016/0042-6822(66)90128-0

10.1017/S0022172400055984

10.1099/0022-1317-4-4-479

10.1017/S0022172400056321

10.1016/0042-6822(58)90010-2

Cowan, 1970, Immunochemical studies of foot-and-mouth disease. VIII. Detection and quantitation of antibodies by radial immunodiffusion, Journal of Immunology, 105, 557, 10.4049/jimmunol.105.2.557

10.1017/S0022172400040146

10.1017/S0022172400023639

Graves, 1960, The differentiation of subtypes (variants) of foot-and-mouth disease virus by serologic methods. I. Complement fixation test, American Journal of Veterinary Research, 21, 687

Graves, 1960, The differentiation of subtypes (variants) of foot-and-mouth disease virus by serologic methods. II. Precipitin test in agar, American Journal of Veterinary Reseasch, 21, 691

Lobo, 1974, Differentiation of type A foot-and-mouth disease virus subtypes by double and radial immunodiffusion analysis, American Journal of Veterinary Research, 35, 1121

10.4267/2042/67015

Martinsen, 1971, Two plaque size variants of foot-and-mouth disease virus differing in neutralization by guinea pig antisera, Research in Veterinary Science, 12, 399, 10.1016/S0034-5288(18)34177-8

Karber, 1931, Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche, Archiv für experimentelle Pathologic und Pharmakologie, 162, 480

Pay T. W. F. & Parker M. J. (1977). Some statistical and experimental design problems in the assessment of FMD vaccine potency. International Symposium on Foot-and-mouth Disease. Lyon, 1976. Developments in Biological Standardization 35, 369–83.

Stellman, 1968, Contrôle quantitatif du vaccin antiaphteux. Analyse statistique de la relation liant les titre d'anticorps neutralisants au pourcentage de protection bovine, Recueil de Médicine Véter'inaire de l'Ecole d'Alfort, 144, 325

10.1099/0022-1317-4-3-313