Micromechanical Testing of Individual Collagen Fibrils

Macromolecular Bioscience - Tập 6 Số 9 - Trang 697-702 - 2006
J.A.J. van der Rijt1, Kees O. van der Werf2, Martin L. Bennink2, Pieter J. Dijkstra1, Jan Feijén1
1Polymer Chemistry and Biomaterials, Institute for Biomedical Technology, Department of Science and Technology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands.
2Biophysical Engineering, MESA+ Institute for Nanotechnology, Department of Science and Technology, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands

Tóm tắt

AbstractSummary: A novel method based on AFM was used to attach individual collagen fibrils between a glass surface and the AFM tip, to allow force spectroscopy studies of these. The fibrils were deposited on glass substrates that are partly coated with Teflon AF®. A modified AFM tip was used to accurately deposit epoxy glue droplets on either end of the collagen fibril that cross the glass‐Teflon AF® interface, as to such attach it with one end to the glass and the other end to the AFM tip. Single collagen fibrils have been mechanically tested in ambient conditions and were found to behave reversibly up to stresses of 90 MPa. Within this regime a Young's modulus of 2–7 GPa was obtained. In aqueous media, the collagen fibrils could be tested reversibly up to about 15 MPa, revealing Young's moduli ranging from 0.2 to at most 0.8 GPa. magnified image

Từ khóa


Tài liệu tham khảo

10.1016/S0021-9290(98)00174-2

Nimni M. E., 1988, Biochemistry

Kastelic J., 1980, Symposium of Society for Experimental Biology – Mechanical Properties of Biological Materials

10.1038/414773a

10.1016/S0006-3495(04)74366-0

10.1016/S0006-291X(02)00685-X

10.1016/j.yexcr.2004.05.022

10.1063/1.1144378

10.1002/(SICI)1097-4628(19960131)59:5<887::AID-APP13>3.0.CO;2-J

10.1002/(SICI)1097-4628(19960926)61:13<2437::AID-APP23>3.0.CO;2-#

10.3233/BIR-1980-171-211

10.1098/rstb.2001.1033

Thông tin
Thông tin xuất bản

Macromolecular Bioscience

Tập 6 Số 9

697-702

Thông tin tác giả