Microdot technology for individual marking of small arthropods

Agricultural and Forest Entomology - Tập 14 Số 2 - Trang 171-175 - 2012
Michael R. Whitehead1, Rod Peakall1
1Evolution, Ecology & Genetics, Research School of Biology, The Australian National University, Canberra ACT 0200, Australia

Tóm tắt

Individual mark–release–recapture is an important method for gathering data on insect movement, although it is limited by the constraints of tagging small insects with individual information.

Microdots, originally developed for covert security applications, are small polymer discs (diameter 0.5 mm) bearing up to 26 characters of information and have the potential as an alternative to the larger bee tags. In the present study, we test microdots for the individual marking of a 9‐mm parasitoid wasp.

We individually marked 505 wasps. The recapture rate was 24% of individuals over 189 recapture events, for which 84% retained legible microdot labels. Movement was in the range 0–161 m with a mean displacement 21.2 ± 2.7 m. A captive survival experiment showed no difference in lifespan between marked and unmarked wasps.

The present study shows that microdots can provide an effective, durable, low‐cost method for individually tagging small insects. The technique offers new opportunities by greatly expanding the capability for individually marking small insects, shifting the minimum size below that of bee tags, which is the only other manufactured option for individualized miniature marking.

Từ khóa


Tài liệu tham khảo

10.1111/j.1570-7458.2005.00320.x

10.1093/aesa/65.1.236

10.1016/j.biocontrol.2009.04.014

10.1603/022.038.0121

10.1111/j.1440-6055.1998.tb01572.x

10.1603/022.038.0319

10.1006/bcon.1996.0005

10.1098/rstb.2003.1299

10.1073/pnas.0900646106

10.1146/annurev.ento.46.1.511

10.1007/b97377

10.1603/EN09243

10.1111/j.1365-2311.1986.tb00317.x

10.1890/08-0973.1

10.1016/j.tplants.2010.09.006

10.1111/j.1570-7458.2010.01031.x

10.1242/jeb.050526

10.1093/ae/50.4.212

10.1371/journal.pone.0019669

10.2307/2389335

10.1111/j.1469-8137.2010.03308.x

10.1046/j.1570-7458.2003.00015.x

R Core Development Team(2011)R: A Language and Environment for Statistical Computing.R Foundation for Statistical Computing Austria [WWW document]. URLhttp://www.R‐project.org.

10.1111/j.1440-6055.1970.tb00789.x

10.1038/379029b0

10.1007/s00265-008-0696-z

10.2307/2269572

10.1046/j.1420-9101.2003.00650.x

10.1111/j.1365-2435.2005.01010.x

10.1126/science.1087835

10.1111/j.0307-6946.2005.00659.x

10.1016/j.cub.2006.11.064

10.1038/433703a

10.1017/S0007485300040815

10.1080/00063659909477239

10.1371/journal.pone.0010738

Wineriter S.A., 1984, Insect marking techniques: durability of materials., Entomological News, 95, 117