Sự xuất hiện của vi sinh vật trong các bể chứa nitơ lỏng: một thách thức cho ngân hàng sinh học?
Tóm tắt
Từ khóa
Tài liệu tham khảo
Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE (2005) Defining the normal bacterial flora of the oral cavity. J Clin Microbiol 43(11):5721–5732. https://doi.org/10.1128/jcm.43.11.5721-5732.2005
Argyle CE, Harper JC, Davies MC (2016) Oocyte cryopreservation: where are we now? Hum Reprod Update 22(4):440–449. https://doi.org/10.1093/humupd/dmw007
Baber R, Kiehntopf M (2019) Automation in biobanking from a laboratory medicine perspective. J Lab Med 43(6):329–338. https://doi.org/10.1515/labmed-2019-0151
Bajerski F, Wagner D, Mangelsdorf K (2017) Cell membrane fatty acid composition of Chryseobacterium frigidisoli PB4(T), isolated from antarctic glacier forefield soils, in response to changing temperature and pH conditions. Front Microbiol 8(677):677. https://doi.org/10.3389/fmicb.2017.00677
Bajerski F, Stock J, Hanf B, Darienko T, Heine-Dobbernack E, Lorenz M, Naujox L, Keller ERJ, Schumacher HM, Friedl T, Eberth S, Mock H-P, Kniemeyer O, Overmann J (2018) ATP content and cell viability as indicators for cryostress across the diversity of life. Front Physiol 9(921):921. https://doi.org/10.3389/fphys.2018.00921
Bajerski F, Bürger A, Glasmacher B, Keller ERJ, Müller K, Mühldorfer K, Nagel M, Rüdel H, Müller T, Schenkel J, Overmann J (2020) Factors determining microbial colonization of liquid nitrogen storage tanks used for archiving biological samples. Appl Microbiol Biotechnol 104(1):131–144. https://doi.org/10.1007/s00253-019-10242-1
Bartels P, Kotze A (2006) Wildlife biomaterial banking in Africa for now and the future. J Environ Monit 8(8):779–781. https://doi.org/10.1039/b602809h
Bielanski A (1997) A review on disease transmission studies in relationship to production of embryos by in vitro fertilization and to related new reproductive technologies. Biotechnol Adv 15(3):633–656. https://doi.org/10.1016/S0734-9750(97)00041-4
Bielanski A (2005) Experimental microbial contamination and disinfection of dry (vapour) shipper dewars designed for short-term storage and transportation of cryopreserved germplasm and other biological specimens. Theriogenology 63(7):1946–1957. https://doi.org/10.1016/j.theriogenology.2004.09.003
Bielanski A, Vajta G (2009) Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units. Hum Reprod 24(10):2457–2467. https://doi.org/10.1093/humrep/dep117
Bielanski A, Nadin-Davis S, Sapp T, Lutze-Wallace C (2000) Viral contamination of embryos cryopreserved in liquid nitrogen. Cryobiology 40(2):110–116
Bielanski A, Bergeron H, Lau PCK, Devenish J (2003) Microbial contamination of embryos and semen during long term banking in liquid nitrogen. Cryobiology 46(2):146–152. https://doi.org/10.1016/S0011-2240(03)00020-8
Bojic S, Murray A, Bentley BL, Spindler R, Pawlik P, Cordeiro JL, Bauer R, De Magalhães JP (2021) Winter is coming: the future of cryopreservation. BMC Biol 19(1). https://doi.org/10.1186/s12915-021-00976-8
Brader G, Compant S, Vescio K, Mitter B, Trognitz F, Ma L-J, Sessitsch A (2017) Ecology and genomic insights into plant-pathogenic and plant-nonpathogenic endophytes. Annu Rev Phytopathol 55(1):61–83. https://doi.org/10.1146/annurev-phyto-080516-035641
Cai H, Niringiyumukiza JD, Li Y, Lai Q, Jia Y, Su P, Xiang W (2018) Open versus closed vitrification system of human oocytes and embryos: a systematic review and meta-analysis of embryologic and clinical outcomes. Reprod Biol Endocrinol 16(1):123. https://doi.org/10.1186/s12958-018-0440-0
Cicek MS, Olson JE (2020) Mini-review of laboratory operations in biobanking: building biobanking resources for translational research. Front Public Health 8:362. https://doi.org/10.3389/fpubh.2020.00362
Clarke AG (2009) The Frozen Ark Project: the role of zoos and aquariums in preserving the genetic material of threatened animals. Int Zoo Yearb 43(1):222–230. https://doi.org/10.1111/j.1748-1090.2008.00074.x
Comizzoli P (2015) Biobanking efforts and new advances in male fertility preservation for rare and endangered species. Asian J Androl 17(4):640–645. https://doi.org/10.4103/1008-682X.153849
Comizzoli P, Wildt DE (2017) Cryobanking biomaterials from wild animal species to conserve genes and biodiversity: relevance to human biobanking and biomedical research. In: Hainaut P, Vaught J, Zatloukal K, Pasterk M (eds) Biobanking of Human Biospecimens: Principles and Practice. Springer International Publishing, Cham, pp 217–235
Compant S, Clément C, Sessitsch A (2010) Plant growth-promoting bacteria in the rhizo- and endosphere of plants: their role, colonization, mechanisms involved and prospects for utilization. Soil Biol Biochem 42(5):669–678. https://doi.org/10.1016/j.soilbio.2009.11.024
Coppola L, Cianflone A, Grimaldi AM, Incoronato M, Bevilacqua P, Messina F, Baselice S, Soricelli A, Mirabelli P, Salvatore M (2019) Biobanking in health care: evolution and future directions. J Transl Med 17(1):172. https://doi.org/10.1186/s12967-019-1922-3
Criado E, Moalli F, Polentarutti N, Albani E, Morreale G, Menduni F, Levi-Setti PE (2011) Experimental contamination assessment of a novel closed ultravitrification device. Fertil Steril 95(5):1777–1779. https://doi.org/10.1016/j.fertnstert.2010.12.044
Dekaboruah E, Suryavanshi MV, Chettri D, Verma AK (2020) Human microbiome: an academic update on human body site specific surveillance and its possible role. Arch Microbiol 202(8):2147–2167. https://doi.org/10.1007/s00203-020-01931-x
DIN (2018) Risikomanagement - Leitlinien (ISO 31000:2018). Beuth, Germany. https://dx.doi.org/10.31030/2880923
DIN EN ISO 20387 (2018) Biotechnologie - Biobanking - allgemeine Anforderungen für Biobanking (ISO 20387:2018): Biotechnology - biobanking - general requirements for biobanking (ISO 20387:2018). Beuth, Germany
Drexler HG, Uphoff CC (2002) Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology 39(2):75–90. https://doi.org/10.1023/A:1022913015916
Eisthen HL, Theis KR (2016) Animal–microbe interactions and the evolution of nervous systems. Philos Trans R Soc Lond B Biol Sci 371(1685):20150052. https://doi.org/10.1098/rstb.2015.0052
Flickinger MC, Smith D (2009) Culture collections and biological resource centres (BRCs). In: Flickinger MC (ed) Encyclopedia of industrial biotechnology. https://doi.org/10.1002/9780470054581.eib246
Fountain D, Ralston M, Higgins N, Gorlin JB, Uhl L, Wheeler C, Antin JH, Churchill WH, Benjamin RJ (1997) Liquid nitrogen freezers: a potential source of microbial contamination of hematopoietic stem cell components. Transfusion 37(6):585–591. https://doi.org/10.1046/j.1537-2995.1997.37697335152.x
Furuta K, Allocca CM, Schacter B, Bledsoe MJ, Ramirez NC (2018) Standardization and innovation in paving a path to a better future: an update of activities in ISO/TC276/WG2 biobanks and bioresources. Biopreserv Biobank 16(1):23–27. https://doi.org/10.1089/bio.2017.0117
García-Bastidas FA, Van der Veen AJT, Nakasato-Tagami G, Meijer HJG, Arango-Isaza RE, Kema GHJ (2019) An improved phenotyping protocol for Panama disease in banana. Front Plant Sci 10(1006). https://doi.org/10.3389/fpls.2019.01006
Georlette D, Blaise V, Collins T, D’Amico S, Gratia E, Hoyoux A, Marx JC, Sonan G, Feller G, Gerday C (2004) Some like it cold: biocatalysis at low temperatures. FEMS Microbiol Rev 28(1):25–42. https://doi.org/10.1016/j.femsre.2003.07.003
Groeneveld LF, Gregusson S, Guldbrandtsen B, Hiemstra SJ, Hveem K, Kantanen J, Lohi H, Stroemstedt L, Berg P (2016) Domesticated animal biobanking: land of opportunity. PLoS Biol 14(7):e1002523. https://doi.org/10.1371/journal.pbio.1002523
Grout BW, Morris GJ (2009) Contaminated liquid nitrogen vapour as a risk factor in pathogen transfer. Theriogenology 71(7):1079–1082. https://doi.org/10.1016/j.theriogenology.2008.12.011
Hacquard S, Garrido-Oter R, González A, Spaepen S, Ackermann G, Lebeis S, McHardy Alice C, Dangl Jeffrey L, Knight R, Ley R, Schulze-Lefert P (2015) Microbiota and host nutrition across plant and animal kingdoms. Cell Host Microbe 17(5):603–616. https://doi.org/10.1016/j.chom.2015.04.009
Hongoh Y (2010) Diversity and genomes of uncultured microbial symbionts in the termite gut. Biosci Biotechnol Biochem 74(6):1145–1151. https://doi.org/10.1271/bbb.100094
Janssens D, Arahal DR, Bizet C, Garay E (2010) The role of public biological resource centers in providing a basic infrastructure for microbial research. Res Microbiol 161(6):422–429. https://doi.org/10.1016/j.resmic.2010.03.009
Joaquim DC, Borges ED, Viana IGR, Navarro PA, Vireque AA (2017) Risk of contamination of gametes and embryos during cryopreservation and measures to prevent cross-contamination. Biomed Res Int 2017:1840417. https://doi.org/10.1155/2017/1840417
Keller ERJ, Senula A, Zanke C, Grübe M, Kaczmarczyk A (2011) Cryopreservation and in vitro culture - state of the art as conservation strategy for genebanks. Acta Hortic (918):99–111. https://doi.org/10.17660/ActaHortic.2011.918.11
Knierim D, Menzel W, Winter S (2017) Analysis of the complete genome sequence of euphorbia ringspot virus, an atypical member of the genus Potyvirus. Arch Virol 162(1):291–293. https://doi.org/10.1007/s00705-016-3087-1
Köpnick C, Grübe M, Stock J, Senula A, Mock H-P, Nagel M (2018) Changes of soluble sugars and ATP content during DMSO droplet freezing and PVS3 droplet vitrification of potato shoot tips. Cryobiology 85:79–86. https://doi.org/10.1016/j.cryobiol.2018.09.005
Larman MG, Hashimoto S, Morimoto Y, Gardner DK (2014) Cryopreservation in ART and concerns with contamination during cryobanking. Reprod Med Biol 13(3):107–117
Livieri T (2011) Black-footed ferret recovery in North America. In: Soorae PS (ed) Global re-introduction perspectives: 2011 more case studies from around the globe. IUCN/SSC Re-introduction Specialist Group Environment Agency, Switzerland, Abu Dhabi, pp 157–164
Malsagova K, Kopylov A, Stepanov A, Butkova T, Sinitsyna A, Izotov A, Kaysheva A (2020) Biobanks—a platform for scientific and biomedical research. Diagnostics 10(7):485
Mendy M, Lawlor RT, van Kappel AL, Riegman PHJ, Betsou F, Cohen OD, Henderson MK (2018) Biospecimens and biobanking in global health. Clin Lab Med 38(1):183–207. https://doi.org/10.1016/j.cll.2017.10.015
Molina I, Mari M, Martínez JV, Novella-Maestre E, Pellicer N, Pemán J (2016) Bacterial and fungal contamination risks in human oocyte and embryo cryopreservation: open versus closed vitrification systems. Fertil Steril 106(1):127–132. https://doi.org/10.1016/j.fertnstert.2016.03.024
Morris GJ (2005) The origin, ultrastructure, and microbiology of the sediment accumulating in liquid nitrogen storage vessels. Cryobiology 50(3):231–238. https://doi.org/10.1016/j.cryobiol.2005.01.005
Morris G, Acton E, Faszer K, Franklin A, Yin H, Bodine R, Pareja J, Zaninovic N, Gosden R (2006) Cryopreservation of murine embryos, human spermatozoa and embryonic stem cells using a liquid nitrogen-free, controlled rate freezer. Reprod Biomed Online 13(3):421–426
Mykytczuk NCS, Foote SJ, Omelon CR, Southam G, Greer CW, Whyte LG (2013) Bacterial growth at −15°C; molecular insights from the permafrost bacterium Planococcus halocryophilus Or1. ISME J 7(6):1211–1226. https://doi.org/10.1038/ismej.2013.8
O’Donnell K, Sharrock S (2017) The contribution of botanic gardens to ex situ conservation through seed banking. Plant Divers 39(6):373–378. https://doi.org/10.1016/j.pld.2017.11.005
OECD O (2009) Guidelines on human biobanks and genetic research databases. Organisation for Economic Co-Operation and Development (OECD) Paris
Overmann J (2013) Principles of enrichment, isolation, cultivation, and preservation of prokaryotes. In: Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F (eds) The prokaryotes: prokaryotic biology and symbiotic associations. Springer, Berlin, Heidelberg, pp 149–207
Overmann J (2015) Significance and future role of microbial resource centers. Syst Appl Microbiol 38(4):258–265. https://doi.org/10.1016/j.syapm.2015.02.008
Overmann J, Schubert K (2002) Phototrophic consortia: model systems for symbiotic interrelations between prokaryotes. Arch Microbiol 177(3):201–208. https://doi.org/10.1007/s00203-001-0377-z
Overmann J, Smith D (2017) Microbial Resource Centers Contribute to Bioprospecting of Bacteria and Filamentous Microfungi. In: Paterson R., Lima N. (eds) Bioprospecting. Topics in Biodiversity and Conservation, vol 16. Springer, Cham. https://doi.org/10.1007/978-3-319-47935-4_4
Panis B, Nagel M, Van den Houwe I (2020) Challenges and prospects for the conservation of crop genetic resources in field genebanks, in in vitro collections and/or in liquid nitrogen. Plants 9(12):1634
Parmegiani L, Accorsi A, Bernardi S, Arnone A, Cognigni GE, Filicori M (2012) A reliable procedure for decontamination before thawing of human specimens cryostored in liquid nitrogen: three washes with sterile liquid nitrogen (SLN2). Fertil Steril 98(4):870–875. https://doi.org/10.1016/j.fertnstert.2012.06.028
Parry-Jones A, Hansen J, Simeon-Dubach D, Bjugn R (2017) Crisis management for biobanks. Biopreserv Biobank 15(3):253–263
Peeling RW, Boeras D, Wilder-Smith A, Sall A, Nkengasong J (2020) Need for sustainable biobanking networks for COVID-19 and other diseases of epidemic potential. Lancet Infect Dis 20(10):e268–e273. https://doi.org/10.1016/S1473-3099(20)30461-8
Pessoa GA, Rubin MIB, Silva CAM, Rosa DCd (2014) Decontamination of naturally contaminated liquid nitrogen storage tanks. Rev Bras Zootec 43(5):244–249. https://doi.org/10.1590/S1516-35982014000500004
Prieto MT, Sanchez-Calabuig MJ, Hildebrandt TB, Santiago-Moreno J, Saragusty J (2014) Sperm cryopreservation in wild animals. Eur J Wildl Res 60(6):851–864. https://doi.org/10.1007/s10344-014-0858-4
Quambusch M, Brümmer J, Haller K, Winkelmann T, Bartsch M (2016) Dynamics of endophytic bacteria in plant in vitro culture: quantification of three bacterial strains in Prunus avium in different plant organs and in vitro culture phases. Plant Cell Tiss Org 126(2):305–317. https://doi.org/10.1007/s11240-016-0999-0
R Core Team (2019) R: A language and environment for statistical computing. Publisher. http://www.R-project.org/
Ramin M, Bürger A, Hörlein A, Kerkau D, von Walcke-Wulffen V, Nicklas W, Schenkel J (2014) Stability of cryopreserved samples of mutant mice. Biopreserv Biobank 12(5):343–350
Reimer LC, Vetcininova A, Carbasse JS, Söhngen C, Gleim D, Ebeling C, Overmann J (2019) BacDive in 2019: bacterial phenotypic data for high-throughput biodiversity analysis. Nucleic Acids Res 47(D1):D631–D636. https://doi.org/10.1093/nar/gky879
Ross R (2012) Guide for conducting risk assessments, special publication (NIST SP). National Institute of Standards and Technology, Gaithersburg. https://doi.org/10.6028/NIST.SP.800-30r1
Roth RR, James WD (1988) Microbial ecology of the skin. Annu Rev Microbiol 42(1):441–464. https://doi.org/10.1146/annurev.mi.42.100188.002301
Santiago-Moreno J, Astorga RJ, Luque I, Coloma MA, Toledano-Díaz A, Pulido-Pastor A, Gómez-Guillamon F, Salas-Vega R, López-Sebastián A (2009) Influence of recovery method and microbial contamination on the response to freezing–thawing in ibex (Capra pyrenaica) epididymal spermatozoa. Cryobiology 59(3):357–362. https://doi.org/10.1016/j.cryobiol.2009.09.012
Saragusty J (2012) Genome banking for vertebrates wildlife conservation. In: Igor I (ed) Current frontiers in cryobiology. IntechOpen, Katkov. https://doi.org/10.5772/35012. Available from: https://www.intechopen.com/chapters/31237
Schafer TW, Everett J, Silver GH, Came PE (1976) Biohazard: virus-contaminated liquid nitrogen. Science 191(4222):24–26. https://doi.org/10.1126/science.191.4222.24-c
Scherling C, Ulrich K, Ewald D, Weckwerth W (2009) A metabolic signature of the beneficial interaction of the endophyte Paenibacillus sp isolate and in vitro-grown poplar plants revealed by metabolomics. Mol Plant Microbe Interact 22(8):1032–1037. https://doi.org/10.1094/Mpmi-22-8-1032
Schüngel M, Smith D, Bizet C, Stackebrandt E, Consortium M (2014) The role of the European microbial resource research infrastructure project. Enliven: Microb Microbial Tech 1(1):001
Sender R, Fuchs S, Milo R (2016) Revised estimates for the number of human and bacteria cells in the body. PLoS Biol 14(8):e1002533–e1002533. https://doi.org/10.1371/journal.pbio.1002533
Senula A, Keller ERJ (2011) Cryopreservation of mint - routine application in a genebank, experience and problems. Acta Hortic:467–475. https://doi.org/10.17660/ActaHortic.2011.908.60
Senula A, Büchner D, Keller ERJ, Nagel M (2018) An improved cryopreservation protocol for Mentha spp. based on PVS3 as the cryoprotectant. Cryo Lett 39(6):345–353
Sharma SK, Kumar R, Vaishnav A (2017) Microbial cultures: maintenance, preservation and registration. In: Varma A, Sharma A (eds) Modern tools and techniques to understand microbes. Springer, Cham. https://doi.org/10.1007/978-3-319-49197-4_22
Sharrock S (2020) Plant Conservation Report 2020: A review of progress in implementation of the Global Strategy for Plant Conservation 2011–2020 Technical Series No 95. Secretariat of the Convention on Biological Diversity, Montréal, Canada and Botanic Gardens Conservation International, Richmond, UK, p 68
Shimkets LJ (2013) Prokaryotic Life Cycles. In: Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F (eds) The prokaryotes: prokaryotic communities and ecophysiology. Springer, Berlin, Heidelberg, pp 317–336
Smith D, Ryan M (2012) Implementing best practices and validation of cryopreservation techniques for microorganisms. Sci World J 2012:805659. https://doi.org/10.1100/2012/805659
Smith D, Fritze D, Stackebrandt E (2013) Public service collections and biological resource centers of microorganisms. In: Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F (eds) The prokaryotes: prokaryotic biology and symbiotic associations. Springer, Berlin, Heidelberg, pp 267–304. https://doi.org/10.1007/978-3-642-30194-0_14
Standard B, Standard NZ (2009) Risk management-Principles and guidelines. BS ISO 31000:2009
Stock J, Mock H-P, Senula A, Nagel M (2018) Arabidopsis - a model to elucidate complex stress response mechanism during cryopreservation. Acta Hortic 1234:85–96. https://doi.org/10.17660/ActaHortic.2019.1234.11
Tao Y, Sanger E, Saewu A, Leveille MC (2020) Human sperm vitrification: the state of the art. Reprod Biol Endocrinol 18(1). https://doi.org/10.1186/s12958-020-00580-5
Tedder RS, Zuckerman MA, Brink NS, Goldstone AH, Fielding A, Blair S, Patterson KG, Hawkins AE, Gormon AM, Heptonstall J, Irwin D (1995) Hepatitis B transmission from contaminated cryopreservation tank. Lancet 346(8968):137–140. https://doi.org/10.1016/S0140-6736(95)91207-X
Ulrich K, Ulrich A, Ewald D (2008) Diversity of endophytic bacterial communities in poplar grown under field conditions. Fems Microbiol Ecol 63(2):169–180. https://doi.org/10.1111/j.1574-6941.2007.00419.x
Uphoff CC, Drexler HG (2013) Detection of mycoplasma contaminations. In: Helgason CD, Miller CL (eds) Basic cell culture protocols. Humana Press, Totowa, pp 1–13
Uphoff CC, Lange S, Denkmann SA, Garritsen HSP, Drexler HG (2015) Prevalence and characterization of murine leukemia virus contamination in human cell lines. PLoS One 10(4):e0125622. https://doi.org/10.1371/journal.pone.0125622
Uragami A, Sakai A, Nagai M, Takahashi T (1989) Survival of cultured cells and somatic embryos of Asparagus officinalis cryopreserved by vitrification. Plant Cell Rep 8(7):418–421. https://doi.org/10.1007/BF00270083
Vajta G, Rienzi L, Ubaldi FM (2015) Open versus closed systems for vitrification of human oocytes and embryos. Reprod Biomed Online 30(4):325–333. https://doi.org/10.1016/j.rbmo.2014.12.012
Vaught JB, Henderson MK (2011) Biological sample collection, processing, storage and information management. IARC Sci Publ. 2011;(163):23-42. PMID: 22997855
Vekeman B, Hoefman S, De Vos P, Spieck E, Heylen K (2013) A generally applicable cryopreservation method for nitrite-oxidizing bacteria. Syst Appl Microbiol 36(8):579–584. https://doi.org/10.1016/j.syapm.2013.07.002
Vitrenko Y, Kostenko I, Kulebyakina K, Duda A, Klunnyk M, Sorochynska K (2017) Fetal tissues tested for microbial sterility by culture- and PCR-based methods can be safely used in clinics. Cell Transplant 26(2):339–350. https://doi.org/10.3727/096368916X692735
Wade WG (2013) The oral microbiome in health and disease. Pharmacol Res 69(1):137–143. https://doi.org/10.1016/j.phrs.2012.11.006
Wang M-R, Lambardi M, Engelmann F, Pathirana R, Panis B, Volk GM, Wang Q-C (2020) Advances in cryopreservation of in vitro-derived propagules: technologies and explant sources. Plant Cell Tiss Org. https://doi.org/10.1007/s11240-020-01770-0
WIEWS (2021) World information and early warning system on plant genetic resources for food and agriculture. PUblisher. http://www.fao.org/wiews/background/en/. Accessed 23rd March 2021
Wilson D (1995) Endophyte: the evolution of a term, and clarification of its use and definition. Oikos 73(2):274–276. https://doi.org/10.2307/3545919
Wolkers WF, Oldenhof H (2021) Principles underlying cryopreservation and freeze-drying of cells and tissues. In: Wolkers WF, Oldenhof H (eds) Cryopreservation and freeze-drying protocols. Springer US, New York, pp 3–25
Wu L, Sun Q, Sugawara H, Yang S, Zhou Y, McCluskey K, Vasilenko A, Suzuki K, Ohkuma M, Lee Y, Robert V, Ingsriswang S, Guissart F, Philippe D, Ma J (2013) Global catalogue of microorganisms (gcm): a comprehensive database and information retrieval, analysis, and visualization system for microbial resources. BMC Genomics 14:933. https://doi.org/10.1186/1471-2164-14-933
Zhang G, Swaisgood RR, Zhang H (2004) Evaluation of behavioral factors influencing reproductive success and failure in captive giant pandas. Zoo Biol 23(1):15–31. https://doi.org/10.1002/zoo.10118