Độ đa dạng vi sinh vật và khả năng cố định nitơ liên quan đến vi khuẩn lam không hình cầu Lyngbya robusta trong môi trường nước ngọt

Springer Science and Business Media LLC - Tập 25 - Trang 1039-1045 - 2012
Jason N. Woodhouse1, Sarah E. Ongley1, Mark V. Brown1, Brett A. Neilan1
1School of Biotechnology and Biomolecular Sciences, The University of New South Wales, Sydney, Australia

Tóm tắt

Hồ Atitlan, Guatemala, một hồ nước ngọt tại Nam Mỹ, gặp phải hiện tượng nở hoa thường xuyên hàng năm do vi khuẩn lam dạng sợi planktic Lyngbya robusta gây ra. Các đặc trưng sinh hóa trước đây của hiện tượng nở hoa đã xác định được hoạt động nitrogenase theo chu kỳ ngày đêm điển hình của vi khuẩn lam không hình cầu, bên cạnh việc phát hiện ở mức thấp các loại độc tố cyanobacterial như cylindrospermopsin và saxitoxin. Một phương pháp phân tử, kết hợp việc giải mã sâu các gen 16S rRNA và nifH, đã được áp dụng cho một mẫu bị chiếm ưu thế bởi vi khuẩn lam được thu thập trong thời gian nở hoa rộng rãi năm 2009. Lyngbya chiếm hơn 60% tổng số chuỗi 16S rRNA, và loài vi khuẩn lam duy nhất khác được phát hiện là picophytoplankton Synechococcus. Cộng đồng vi khuẩn còn lại gồm các sinh vật điển hình của các nguồn nước ngọt giàu dinh dưỡng khác, mặc dù tỷ lệ phong phú là không điển hình. Một sinh vật kị khí bắt buộc Opitutus, không thường được tìm thấy trong các hệ sinh thái nước ngọt, đã được xác định trong cộng đồng, điều này gợi ý rằng nó có thể có vai trò trong việc tăng cường khả năng cố định nitơ. Khả năng cố định nitơ chính được quy cho Lyngbya, với các sinh vật có khả năng cố định nitơ khác, Desulfovibrio, Clostridium và Methylomonas, có mặt với số lượng rất thấp.

Từ khóa

#Hồ Atitlan; vi khuẩn lam; Lyngbya robusta; cố định nitơ; bông nở; sinh học phân tử; vi sinh vật nước ngọt

Tài liệu tham khảo

Abed RMM, Zein B, Al-Thukair A, De Beer D (2007) Phylogenetic diversity and activity of aerobic heterotrophic bacteria from a hypersaline oil-polluted microbial mat. Syst Appl Microbiol 30:319–330 Albertano P, Urzi C (1999) Structural interactions among epilithic cyanobacteria and heterotrophic microorganisms in Roman Hypogea. Microb Ecol 38:244–252 Al-Tebrineh J, Pearson LA, Yasar SA, Neilan BA (2012) A multiplex qPCR targeting hepato- and neurotoxigenic cyanobacteria of global significance. Harmful Algae 15:19–25 Becker S, Singh AK, Postius C, Böger P, Ernst A (2004) Genetic diversity and distribution of periphytic Synechococcus spp. in biofilms and picoplankton of Lake Constance. FEMS Microbiol Ecol 49:181–190 Burke C, Thomas T, Lewis M, Steinberg P, Kjelleberg S (2011) Composition, uniqueness and variability of the epiphytic bacterial community of the green alga Ulva australis. ISME J 5:590–600 Caskey WH, Tiedje JM (1980) The reduction of nitrate to ammonium by a Clostridium sp. isolated from soil. J Gen Microbiol 119:217–223 Chin KJ, Liesack W, Janssen PH (2001) Opitutus terrae gen. nov., sp. nov., to accommodate novel strains of the division ‘Verrucomicrobia’ isolated from rice paddy soil. Int J Syst Evol Microbiol 51:1965–1968 Dupraz C, Visscher PT (2005) Microbial lithification in marine stromatolites and hypersaline mats. Trends Microbiol 13:429–438 Eiler A, Bertilsson S (2004) Composition of freshwater bacterial communities associated with cyanobacterial blooms in four Swedish lakes. Environ Microbiol 6:1228–1243 Engene N, Cameron Coates R, Gerwick WH (2010) 16S rRNA gene heterogeneity in the filamentous marine cyanobacterial genus Lyngbya. J Phycol 46:591–601 Ferris MJ, Ruff-Roberts AL, Kopczynski ED, Bateson MM, Ward DM (1996) Enrichment culture and microscopy conceal diverse thermophilic Synechococcus populations in a single hot spring microbial mat habitat. Appl Environ Microbiol 62:1045–1050 Fründ C, Cohen Y (1992) Diurnal cycles of sulfate reduction under oxic conditions in cyanobacterial mats. Appl Environ Microbiol 58:70–77 Ho L, Hoefel D, Saint CP, Newcombe G (2007) Isolation and identification of a novel microcystin-degrading bacterium from a biological sand filter. Water Res 41:4685–4695 Houmard J, Bogusz D, Bigault R, Elmerich C (1980) Characterization and kinetics of the biosynthesis of some nitrogen fixation (nif) gene products in Klebsiella pneumoniae. Biochimie 62:267–275 Jones AC, Monroe EA, Podell S, Hess WR, Klages S, Esquenazi E, Niessen S, Hoover H, Rothmann M, Lasken RS (2011) Genomic insights into the physiology and ecology of the marine filamentous cyanobacterium Lyngbya majuscula. Proc Natl Acad Sci U S A 108:8815–8820 Jungblut AD, Neilan BA (2009) NifH gene diversity and expression in a microbial mat community on the McMurdo Ice Shelf, Antarctica. Antarct Sci 22:117–122 Klausmeier CA, Litchman E, Daufresne T, Levin SA (2004) Optimal nitrogen-to-phosphorus stoichiometry of phytoplankton. Nature 429:171–174 Leitao E, Oxelfelt F, Oliveira P, Moradas-Ferreira P, Tamagnini P (2005) Analysis of the hupSL operon of the nonheterocystous cyanobacterium Lyngbya majuscula CCAP 1446/4: regulation of transcription and expression under a light–dark regimen. Appl Environ Microbiol 71:4567–4576 Levich A (1996) The role of nitrogen-phosphorus ratio in selecting for dominance of phytoplankton by cyanobacteria or green algae and its application to reservoir management. J Aquat Ecosyst Stress Recover 5:55–61 Li N, Zhang L, Li F, Wang Y, Zhu Y, Kang H, Wang S, Qin S (2011) Metagenome of microorganisms associated with the toxic Cyanobacteria Microcystis aeruginosa analyzed using the 454 sequencing platform. Chin J Oceanol Limnol 29:505–513 Manage PM, Edwards C, Singh BK, Lawton LA (2009) Isolation and identification of novel microcystin-degrading bacteria. Appl Environ Microbiol 75:6924–6928 Neilan BA, Burns BP, Relman DA, Lowe DR (2002) Molecular identification of cyanobacteria associated with stromatolites from distinct geographical locations. Astrobiology 2:271–280 Oh S, Caro-Quintero A, Tsementzi D, Deleon-Rodriguez N, Luo C, Poretsky R, Konstantinidis KT (2011) Metagenomic insights into the evolution, function, and complexity of the planktonic microbial community of Lake Lanier, a temperate freshwater ecosystem. Appl Environ Microbiol 77:6000–6011 Omoregie EO, Crumbliss LL, Bebout BM, Zehr JP (2004) Determination of nitrogen-fixing phylotypes in Lyngbya sp. and Microcoleus chthonoplastes cyanobacterial mats from Guerrero Negro, Baja California, Mexico. Appl Environ Microbiol 70:2119–2128 Paerl HW (1996) A comparison of cyanobacterial bloom dynamics in freshwater, estuarine and marine environments. Phycologia 35:25–35 Paerl HW, Bebout BM, Prufert LE (1989) Bacterial associations with marine Oscillatoria sp. (Trichodesmium sp.) populations: ecophysiological implications. J Phycol 25:773–784 Paerl HW, Prufert LE, Ambrose WW (1991) Contemporaneous N(2) fixation and oxygenic photosynthesis in the nonheterocystous mat-forming cyanobacterium Lyngbya aestuarii. Appl Environ Microbiol 57:3086–3092 Pearson LA, Neilan BA (2008) The molecular genetics of cyanobacterial toxicity as a basis for monitoring water quality and public health risk. Curr Opin Biotechnol 19:281–288 Pearson LA, Moffitt MC, Ginn HP, Neilan BA (2008) The molecular genetics and regulation of cyanobacterial peptide hepatotoxin biosynthesis. Crit Rev Toxicol 38:847–856 Pope PB, Patel BKC (2008) Metagenomic analysis of a freshwater toxic cyanobacteria bloom. FEMS Microbiol Ecol 64:9–27 Prakash S, Lawton LA, Edwards C (2009) Stability of toxigenic Microcystis blooms. Harmful Algae 8:377–384 Rapala J, Berg KA, Lyra C, Niemi RM, Manz W, Suomalainen S, Paulin L, Lahti K (2005) Paucibacter toxinivorans gen. nov., sp. nov., a bacterium that degrades cyclic cyanobacterial hepatotoxins microcystins and nodularin. Int J Syst Evol Microbiol 55:1563–1568 Reid RP, Visscher PT, Decho AW, Stolz JF, Bebout BM, Dupraz C, Macintyre IG, Paerl HW, Pinckney JL, Prufert-Bebout L, Steppe TF, Desmarais DJ (2000) The role of microbes in accretion, lamination and early lithification of modern marine stromatolites. Nature 406:989–992 Rejmánková E, Komárek J, Dix M, Komárková J, Girón N (2011) Cyanobacterial blooms in Lake Atitlan, Guatemala. Limnologica 41:296–302 Rinta-Kanto JM, Ouellette AJA, Boyer GL, Twiss MR, Bridgeman TB, Wilhelm SW (2005) Quantification of toxic Microcystis spp. during the 2003 and 2004 blooms in western Lake Erie using quantitative real-time PCR. Environ Sci Technol 39:4198–4205 Sánchez O, Diestra E, Esteve I, Mas J (2005) Molecular characterization of an oil-degrading cyanobacterial consortium. Microb Ecol 50:580–588 Sandh G, Xu L, Bergman B (2012) Diazocyte development in the marine diazotrophic cyanobacterium Trichodesmium. Microbiology 158:345–352 Sañudo-Wilhelmy SA, Kustka AB, Gobler CJ, Hutchins DA, Yang M, Lwiza K, Burns J, Capone DG, Raven JA, Carpenter EJ (2001) Phosphorus limitation of nitrogen fixation by Trichodesmium in the central Atlantic Ocean. Nature 411:66–69 Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, Sahl JW, Stres B, Thallinger GG, Van Horn DJ, Weber CF (2009) Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75:7537–7541 Sivonen K, Niemelä S, Niemi R, Lepistö L, Luoma T, Räsänen L (1990) Toxic cyanobacteria (blue-green algae) in Finnish fresh and coastal waters. Hydrobiologia 190:267–275 Slobodkin A, Verstraete W (1993) Isolation and characterization of Veillonella sp. from methanogenic granular sludge. Appl Microbiol Biotechnol 39:649–653 Steppe TF, Paerl HW (2005) Nitrogenase activity and nifH expression in a marine intertidal microbial mat. Microb Ecol 49:315–324 Steppe TF, Olson JB, Paerl HW, Litaker RW, Belnap J (1996) Consortial N2 fixation: a strategy for meeting nitrogen requirements of marine and terrestrial cyanobacterial mats. FEMS Microbiol Ecol 21:149–156 Stevenson BS, Waterbury JB (2006) Isolation and identification of an epibiotic bacterium associated with heterocystous Anabaena cells. Biol Bull 210:73–77 Suda S, Liu Y, He J, Hu Z, Hiroki M, Watanabe MM (1998) Morphological, biochemical and physiological characteristics of Lyngbya hieronymusii var. hieronymusii (Oscillatoriales, Cyanobacteria). Phycol Res 46:51–55 Tan LT (2007) Bioactive natural products from marine cyanobacteria for drug discovery. Phytochemistry (Oxf) 68:954–979 Teske A, Ramsing NB, Habicht K, Fukui M, Küver J, Jørgensen BB, Cohen Y (1998) Sulfate-reducing bacteria and their activities in cyanobacterial mats of Solar Lake (Sinai, Egypt). Appl Environ Microbiol 64:2943–2951 Visscher PT, Reid RP, Bebout BM, Hoeft ES, Macintyre IG, Thompson JA (1998) Formation of lithified micritic laminae in modern marine stromatolites (Bahamas): the role of sulfur cycling. Am Mineral 83:1482–93 Ward DM, Ferris MJ, Nold SC, Bateson MM (1998) A natural view of microbial biodiversity within hot spring cyanobacterial mat communities. Microbiol Mol Biol Rev 62:1353–1370 Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173:697–703 Wilhelm SW, Farnsley SE, Lecleir GR, Layton AC, Satchwell MF, Debruyn JM, Boyer GL, Zhu G, Paerl HW (2011) The relationships between nutrients, cyanobacterial toxins and the microbial community in Taihu (Lake Tai), China. Harmful Algae 10:207–215 Wobus A, Bleul C, Maassen S, Scheerer C, Schuppler M, Jacobs E, Röske I (2003) Microbial diversity and functional characterization of sediments from reservoirs of different trophic state. FEMS Microbiol Ecol 46:331–347 Zehr JP, Mellon M, Braun S, Litaker W, Steppe T, Paerl HW (1995) Diversity of heterotrophic nitrogen fixation genes in a marine cyanobacterial mat. Appl Environ Microbiol 61:2527–2532