Microbial availability of mercury: effective detection and organic ligand effect using a whole-cell bioluminescent bioreporter

Ecotoxicology - Tập 24 - Trang 2200-2206 - 2015
Xianghua Xu1,2, Kathryn Oliff2, Tingting Xu2, Steven Ripp2, Gary Sayler2, Jie Zhuang3
1Jiangsu Key Laboratory of Agricultural Meteorology, Nanjing University of Information Science & Technology, Nanjing, China
2The Center for Environmental Biotechnology, The University of Tennessee, Knoxville, USA
3Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, China

Tóm tắt

A luxCDABE-based genetically engineered bacterial bioreporter (Escherichia coli ARL1) was used to detect bioavailable ionic mercury (Hg(II)) and investigate the effects of humic acids and ethylenediaminetetraacetic acid (EDTA) on the bioavailability of mercury in E. c oli. Results showed that the E. c oli ARL1 bioreporter was sensitive to mercury, with a detection limit of Hg(II) of 0.5 µg/L and a linear dose/response relationship up to 2000 µg Hg(II)/L. Humic acids and EDTA decreased the Hg(II)-induced bioluminescent response of strain ARL1, suggesting that the two organic ligands reduced the bioavailability of Hg(II) via complexation with Hg(II). Compared with traditional chemical methods, the use of E. c oli ARL1 is a cost-effective, rapid, and reliable approach for measuring aqueous mercury at very low concentrations and thus has potential for applications in field in situ monitoring.

Tài liệu tham khảo

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