Micro-Computed Tomography for Hemorrhage Disruption of Mouse Brain Vasculature

Translational Stroke Research - Tập 3 - Trang 174-179 - 2012
Bohua Xie1,2, Peng Miao1,3, Yuhao Sun1,4, Yongting Wang1, Guo-Yuan Yang1,5,6
1School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University, Shanghai, China
2School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, China
3School of Communication and Information Engineering, Shanghai University, Shanghai, China
4Department of Neurosurgery, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
5Department of Neurology, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China
6Neuroscience and Neuroengineering Center, Med-X Research Institute and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China

Tóm tắt

The use of genetic engineering to develop important neuropathological mouse models has made cerebrovascular imaging essential for the investigation of numerous brain disorders, especially cerebrovascular disorders, such as aneurysms, arteriovenous malformations, and ischemic and hemorrhagic stroke. New laboratory-based X-ray microimagers exist that provide easy access, reliable operation, and performance previously found only in synchrotron-based instruments. Here, we reported a novel approach using such a system to detect intracerebral hemorrhage and resultant cerebrovascular pathology. Adult male C57BL/6 mice (n = 12) underwent 30 μl autologous blood injection into the right basal ganglia region. After sacrificing the animals and vascular perfusion with Microfil® MV-122 Yellow to opacify vascular and microvascular structures, the brain was post-fixed and partially hydrated for 3D imaging with a MicroXCT-400® at 30 KeV and 2-μm resolution. Tomographic reconstruction of high-resolution microimages was accomplished with Amira® software. High-quality 3D images included cerebrocortical microvessels, the circle of Willis, the sagittal sinus, transverse sinus, and other arterial and venous systems. In the ipsilateral hemisphere, there clearly were early-stage vasodilatation and later-stage neovascularization. Very high-resolution, laboratory-based, X-ray micro-CT contrast imaging can accomplish sensitive quantifications of normal and pathological small cerebrovascular changes, especially in hemorrhagic stroke and subsequent hemorrhage-induced neovascularization.

Tài liệu tham khảo

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Translational Stroke Research

Tập 3

174-179

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