Mice lacking melanin-concentrating hormone are hypophagic and lean

Flier, J. S. & Maratos-Flier, E. Obesity and the hypothalamus: novel peptides for new pathways. Cell 92, 437–440 (1998).

Dryden, S., Frankish, H., Wang, Q. & Williams, G. Neuropeptide Y and energy balance: one way ahead for the treatment of obesity? Eur. J. Clin. Invest. 24, 293–308 (1994).

Qu, D.et al. Arole for melanin-concentrating hormone in the central regulation of feeding behaviour. Nature 380, 243–247 (1996).

Sakurai, T.et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell 92, 573–585 (1998).

de Lecea, L.et al. The hypocretins: hypothalamus-specific peptides with neuroexcitatory activity. Proc. Natl Acad. Sci. USA 95, 322–327 (1998).

Erickson, J., Clegg, K. & Palmiter, R. Sensitivity to leptin and susceptibility to seizures of mice lacking neuropeptide Y. Nature 381, 415–418 (1996).

Zhang, Y.et al. Positional cloning of the mouse ob gene and its human homologue. Nature 372, 425–432 (1994).

Huszar, D.et al. Targeted disruption of the melanocortin-4 receptor results in obesity in mice. Cell 88, 131–141 (1997).

Krezel, W.et al. Impaired locomotion and dopamine signaling in retinoid receptor mutant mice. Science 279, 863–867 (1998).

Ahima, R. S., Prabakaran, D. & Flier, J. S. Postnatal leptin surge and regulation of circadian rhythm of leptin by feeding. Implications for energy homeostasis and neuroendocrine function. J. Clin. Invest. 101, 1020–1027 (1998).

Halaas, J.et al. Weight reducing effect of the plasma protein encoded by the obese gene. Science 269, 543–546 (1995).

Ollmann, M. M.et al. Antagonism of central melanocortin receptors in vitro and in vivo by Agouti-related protein. Science 278, 135–138 (1997).

Shutter, J. R.et al. Hypothalamic expression of ART, a novel gene related to agouti, is up-regulated in obese and diabetic mutant mice. Genes Dev. 11, 593–602 (1997).

Tsujii, S. & Bray, G. A. Acetylation alters the feeding response to MSH and beta-endorphin. Brain Res. Bull. 23, 165–169 (1989).

Fan, W., Boston, B. A., Kesterson, R. A., Hruby, V. J. & Cone, R. D. Role of melanocortinergic neurons in feeding and the agouti obesity syndrome. Nature 385, 165–168 (1997).

Thornton, J. E., Cheung, C. C., Clifton, D. K. & Steiner, R. A. Regulation of hypothalamic roopiomelanocortin mRNA by leptin in ob/ob mice. Endocrinology 138, 5063–5066 (1997).

Mizuno, T. M.et al. Hypothalamic pro-opiomelanocortin mRNA is reduced by fasting in ob/ob and db/db mice, but is stimulated by leptin. Diabetes 47, 294–297 (1998).

Ahima, R. S.et al. Role of leptin in the neuroendocrine response to fasting. Nature 382, 250–252 (1996).

Bittencourt, J. C & Elias, C. F. Diencephalic origins of melanin-concentrating hormone immunoreactive projections to medial septum/diagonal band complex and spinal cord using two retrograde fluorescent tracers. Ann. NY Acad. Sci. 680, 462–465 (1993).

Elias, C. F.et al. Chemically defined projections linking the mediobasal hypothalamus and the lateral hypothalamic area. J. Comp. Neurol.(in the press).

Krude, H.et al. Severe early-onset obesity, adrenal insufficiency and red hair pigmentation caused by POMC mutations in humans. Nature Genet. 19, 155–157 (1998).

Nahon, J. L. The melanin-concentrating hormone: from the peptide to the gene. Crit. Rev. Neurobiol. 8, 221–262 (1994).

Rossi, M.et al. Melanin-concentrating hormone acutely stimulates feeding, but chronic administration has no effect on body weight. Endocrinology 138, 351–355 (1997).

Bittencourt, J. C.et al. The melanin-concentrating hormone system of the rat brain: an immuno- and hybridization histochemical characterization. J. Comp. Neurol. 319, 218–245 (1992).

Montague, C. T.et al. Congenital leptin deficiency is associated with severe early-onset obesity in humans. Nature 387, 903–908 (1997).

Breton, C., Presse, F., Hervieu, G. & Nahon, J. L. Structure and regulation of the mouse melanin concentrating hormone mRNA and gene. Mol. Cell. Neurosci. 4, 271–284 (1993).

Li, E., Sucov, H. M., Lee, K. F., Evans, R. M. & Jaenisch, R. Normal development and growth of mice carrying a targeted disruption of the alpha 1 retinoic acid receptor gene. Proc. Natl Acad. Sci. USA 90, 1590–1594 (1993).

Marks, D. L.et al. Simultaneous visualization of two cellular mRNA species in individual neurons by use of a new double in situ hybridization method. Mol. Cell. Neurosci. 3, 395–405 (1993).

Lowell, B. B.et al. Development of obesity in transgenic mice after genetic ablation of brown adipose tissue. Nature 366, 740–742 (1993).