Methanosarcina spelaei sp. nov., a methanogenic archaeon isolated from a floating biofilm of a subsurface sulphurous lake

International Journal of Systematic and Evolutionary Microbiology - Tập 64 Số Pt_10 - Trang 3478-3484 - 2014
Lars Ganzert1, Janosch Schirmack2, Mashal Alawi3, Kai Mangelsdorf4, Wolfgang Sand5, Alexandra Hillebrand‐Voiculescu6, Dirk Wagner3
1Finnish Forest Research Institute, Rovaniemi Unit, Eteläranta 55, 96100 Rovaniemi, Finland
2Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Research Unit Potsdam, Telegrafenberg A45, 14473, Potsdam, Germany
3GFZ German Research Center for Geosciences, Section 4.5 Geomicrobiology, Telegrafenberg, Potsdam, Germany
4GFZ German Research Center for Geosciences, Section 4.3 Organic Geochemistry, Telegrafenberg, Potsdam, Germany
5University of Duisburg-Essen, Biofilm Centre, Aquatic Biotechnology, Geibelstraße 41, 47057 Duisburg, Germany
6Emil Racoviţă Institute of Speleology, Str. Frumoasă, nr. 31, sect. 1, Bucharest, Romania

Tóm tắt

A novel methanogenic archaeon, strain MC-15T, was isolated from a floating biofilm on a sulphurous subsurface lake in Movile Cave (Mangalia, Romania). Cells were non-motile sarcina-like cocci with a diameter of 2–4 µm, occurring in aggregates. The strain was able to grow autotrophically on H2/CO2. Additionally, acetate, methanol, monomethylamine, dimethylamine and trimethylamine were utilized, but not formate or dimethyl sulfide. Trypticase peptone and yeast extract were not required for growth. Optimal growth was observed at 33 °C, pH 6.5 and a salt concentration of 0.05 M NaCl. The predominant membrane lipids of MC-15Twere archaeol and hydroxyarchaeol phosphatidylethanolamine, phosphatidylglycerol, and phosphatidylinositol as well as hydroxyarchaeol phosphatidylserine and archaeol glycosaminyl phosphatidylinositol. The closely related species,Methanosarcina vacuolataandMethanosarcina horonobensis,had a similar composition of major membrane lipids to strain MC-15T. The 16S rRNA gene sequence of strain MC-15Twas similar to those ofMethanosarcina vacuolataDSM 1232T(sequence similarity 99.3 %),Methanosarcina horonobensisHB-1T(98.8 %),Methanosarcina barkeriDSM 800T(98.7 %) andMethanosarcina siciliaeT4/MT(98.4 %). DNA–DNA hybridization revealed 43.3 % relatedness between strain MC-15TandMethanosarcina vacuolataDSM 1232T. The G+C content of the genomic DNA was 39.0 mol%. Based on physiological, phenotypic and genotypic differences, strain MC-15Trepresents a novel species of the genusMethanosarcina, for which the nameMethanosarcinaspelaeisp. nov. is proposed. The type strain is MC-15T( = DSM 26047T = JCM 18469T).

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International Journal of Systematic and Evolutionary Microbiology

Tập 64 Số Pt_10

3478-3484

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