Methamphetamine Enhances HIV-1 Infectivity in Monocyte Derived Dendritic Cells
Tóm tắt
The US is currently experiencing an epidemic of methamphetamine (Meth) use as a recreational drug. Recent studies also show a high prevalence of HIV-1 infection among Meth users. We report that Meth enhances HIV-1 infectivity of dendritic cells as measured by multinuclear activation of a galactosidase indicator (MAGI) cell assay, p24 assay, and LTR-RU5 amplification. Meth induces increased HIV-1 infection in association with an increase in the HIV-1 coreceptors, CXCR4 and CCR5, and infection is mediated by downregulation of extracellular-regulated kinase (ERK2) and the upregulation of p38 mitogen-activated protein kinase (MAPK). A p38 inhibitor (SB203580) specifically reversed the Meth-induced upregulation of the CCR5 HIV-1 coreceptor. The dopamine D2 receptor antagonist RS ± sulpiride significantly reversed the Meth-induced upregulation of CCR5, demonstrating that the Meth-induced effect is mediated via the D2 receptor. These studies report for the first time that Meth fosters HIV-1 infection, potentially via upregulating coreceptor gene expression. Further, Meth mediates its regulatory effects via dopamine receptors and via downregulating ERK2 with a reciprocal upregulation of p38 MAPK. Elucidation of the role of Meth in HIV-1 disease susceptibility and the mechanism through which Meth mediates its effects on HIV-1 infection may help to devise novel therapeutic strategies against HIV-1 infection in high-risk Meth-using HIV-1-infected subjects.
Tài liệu tham khảo
Baselt RC (1982) Disposition of toxic drugs and chemicals in man, vol. 38. 2nd edn. Biomedical Publications, Davis, p 113
Blauvelt A, Asada HM, Saville W, Klaus-Kovtun V, Altman DJ, Yarchoan R (1997) Productive infection of dendritic cells by HIV-1 and their ability to capture virus are mediated through separate pathways. J Clin Invest 100:2043–2053 doi:10.1172/JCI119737
Cameron P, Pope M, Granelli-Piperno A, Steinman RM (1996) Dendritic cells and the replication of HIV-1. J Leukoc Biol 59:158–171
Cao H, Verge V, Baron C, Martinache C, Leon A, Scholl S (2000) In vitro generation of dendritic cells from human blood monocytes in experimental conditions compatible for in vivo cell therapy. J Hematother Stem Cell Res 9:183–194 doi:10.1089/152581600319397
Caux C, Massacrier C, Vanbervliet B, Dubois B, Van Kooten C, Durand I (1994) Activation of human DC through CD40 cross-linking. J Exp Med 180:1263–1272 doi:10.1084/jem.180.4.1263
Chackerian B, Long EM, Luciw PA, Overbaugh J (1997) Human immunodeficiency virus type 1 coreceptors participate in postentry stages in the virus replication cycle and function in simian immunodeficiency virus infection. J Virol 71:3932–3939
Chomczynski P, Sacchi N (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162:156–159 doi:10.1016/0003-2697(87)90021-2
Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W (eds) (1991) In: Current protocols in immunology. Wiley, New York, pp 145–186 ISBN:0-471-52276-7
Dauer M, Obermaier B, Herten J, Haerle C, Pohl K, Rothenfusser S (2003) Mature dendritic cells derived from human monocytes within 48 hours: a novel strategy for dendritic cell differentiation from blood precursors. J Immunol 170:4069–4076
Gavrilin MA, Mathes IE, Podell M (2002) Methamphetamine enhances cell-associated feline immunodeficiency virus replication in astrocytes. J Neurovirol 8:240–249 doi:10.1080/13550280290049660
Geijtenbeek TB, Kwon DS, Torensma R, van Vliet SJ, van Duijnhoven GC, Middel J, Cornelissen IL, Nottet HS, KewalRamani VN, Littman DR, Figdor CG, van Kooyk Y (2000) DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans-infection of T cells. Cell 100:587–597 doi:10.1016/S0092-8674(00)80694-7
Granelli-Piperno A, Moser B, Pope M, Chen D, Wei Y, Isdell F (1996) Efficient interaction of HIV-1 with purified dendritic cells via multiple chemokine coreceptors. J Exp Med 184:2433–2438 doi:10.1084/jem.184.6.2433
Iritani BJ, Hallfors DD, Bauer DJ (2007) Crystal methamphetamine use among young adults in the USA. Addiction 102:1102–1113 doi:10.1111/j.1360-0443.2007.01847.x
Jacque JM, Mann A, Enslen H, Sharova N, Brichacek B, Davis RJ (1998) Modulation of HIV-1 infectivity by MAPK, a virion-associated kinase. EMBO J 17:2607–2618 doi:10.1093/emboj/17.9.2607
Langhoff E, Terwilliger EF, Bos HJ, Kalland KH, Poznansky MC, Bacon OML (1991) Replication of human immunodeficiency virus type 1 in primary dendritic cell cultures. Proc Natl Acad Sci U S A 88:7998–8002 doi:10.1073/pnas.88.18.7998
Larsen KE, Fon EA, Hastings TG, Edwards RH, Sulzer D (2002) Methamphetamine-induced degeneration of dopaminergic neurons involves autophagy and upregulation of dopamine synthesis. J Neurosci 22:8951–8960
Macatonia SE, Patterson S, Knight SC (1989) Suppression of immune responses by dendritic cells infected with HIV. Immunology 67:285–289
Munzar P, Goldberg SR (2000) Dopaminergic involvement in the discriminative-stimulus effects of methamphetamine in rats. Psychopharmacology (Berl) 148:209–216 doi:10.1007/s002130050044
Nair MP, Mahajan SD, Schwartz SA, Reynolds J, Whitney R, Bernstein Z (2005) Cocaine modulates dendritic cell-specific C type intercellular adhesion molecule-3-grabbing nonintegrin expression by dendritic cells in HIV-1 patients. J Immunol 174:6617–6626
National Drug Threat Assessment (NDTA) (2005) Summary report 2005. Methamphetamine. www.usdoj.gov/ndic/pubs11/13846/meth.htm
National Drug Threat Assessment (NDTA) (2007) Methamphetamine. http://www.usdoj.gov/ndic/pubs25/25921/meth.htm#Top
National Survey on Drug Use and Health (NSDUH) (2003) https://nsduhweb.rti.org
NIDA (National Institute of Drug abuse) INFO FACTS (2005) Methamphetamine. National Institute of Drug Abuse, Baltimore, May 2005
Office of National Drug Control Policy (ONDCP) (2007) Drug facts, methamphetamine. http://www.whitehousedrugpolicy.gov/drugfact/methamphetamine/index.html#go3
Pope M, Betjes MGH, Romani N, Cameron PU, Hoffman L, Gezelter S (1994) Conjugates of dendritic cells and memory T lymphocytes from skin facilitate productive infection with HIV-1. Cell 78:389–398 doi:10.1016/0092-8674(94)90418-9
Popik W, Hesselgesser E, Pitha PM (1998) Binding of human immunodeficiency virus type 1 to CD4 and CXCR4 receptors differentially regulates expression of inflammatory genes and activates the MEK/ERK signaling pathway. J Virol 72:6406–6413
Schepers RJF, Oyler JM, Joseph RE, Cone EJ, Moolchan ET, Huestis MA (2003) Methamphetamine and amphetamine pharmacokinetics in oral fluid and plasma after controlled oral methamphetamine administration to human volunteers. Clin Chem 49:121–132 doi:10.1373/49.1.121
Shively L, Chang L, LeBon JM, Liu Q, Riggs AD, Singer-Sam J (2003) Real-time PCR assay for quantitative mismatch detection. Biotechniques 34:498–502
Takayasu T, Ohshima T, Nishigami J, Kondo T, Nagaon T (1995) Screening and determination of methamphetamine and amphetamine in the blood, urine and stomach contents in emergency medical care and autopsy cases. J Clin Forensic Med 2:25–33 doi:10.1016/1353-1131(95)90036-5
Watanabe H, Sekihara S, Nomura Y (1989) Effect of dopamine receptor antagonist on in vivo dopamine release induced by intrastriatal perfusion with methamphetamine in freely moving rats. Methods Find Exp Clin Pharmacol 11:81–85
Weissman D, Li Y, Orenstein JM, Fauci AS (1995) Both a precursor and a mature population of dendritic cells can bind HIV. However, only the mature population that expresses CD80 can pass infection to unstimulated CD4+ T cells. J Immunol 155:4111–4117
Yang X, Gabuzda D (1999) Regulation of human immunodeficiency virus type 1 infectivity by the ERK mitogen-activated protein kinase signaling pathway. J Virol 73:3460–3466
Zhou LJ, Tedder TF (1996) CD14+ blood monocytes can differentiate into functionally mature CD83+ dendritic cells. Proc Natl Acad Sci U S A 93:2588–2592 doi:10.1073/pnas.93.6.2588