Metagenomic analysis on seasonal microbial variations of activated sludge from a full‐scale wastewater treatment plant over 4 years

Environmental Microbiology Reports - Tập 6 Số 1 - Trang 80-89 - 2014
Feng Ju1, Feng Guo1, Lin Ye1, Yu Xia1, Tong Zhang1
1Environmental Biotechnology Lab, The University of Hong Kong SAR, Hong Kong, China

Tóm tắt

SummaryMetagenomic technique was employed to characterize the seasonal dynamics of activated sludge (AS) communities in a municipal wastewater treatment plant (WWTP) over 4 years. The results indicated that contrary to Eukaryota (mainly Rotifera and Nematoda), abundances of Bacteria and Archaea (mainly Euryarchaeota) were significantly higher in winter than summer. Two‐way analysis of variance and canonical correspondence analysis revealed that many functionally important genera followed strong seasonal variation patterns driven by temperature and salinity gradients; among them, two nitrifying bacteria, Nitrospira and Nitrosomonas, displayed much higher abundances in summer, whereas phosphate‐removing genus Tetrasphaera, denitrifier Paracoccus and potential human faecal bacteria, i.e. Bifidobacterium, Dorea and Ruminococcus, showed significantly higher abundances in winter. Particularly, occurrence of dual variation patterns beyond explanation merely by seasonality indicated that multivariables (e.g. dissolved oxygen, sludge retention time, nutrients) participated in shaping AS community structure. However, SEED subsystems annotation showed that functional categories in AS showed no significant difference between summer and winter, indicating that compared with its microbial components, the functional profiles of AS were much more stable. Taken together, our study provides novel insights into the microbial community variations in AS and discloses their correlations with influential factors in WWTPs.

Từ khóa


Tài liệu tham khảo

10.1016/S0022-2836(05)80360-2

10.1038/ismej.2011.119

10.1007/s00253-011-3428-7

10.1128/AEM.71.8.4679-4689.2005

10.1101/gr.104521.109

10.1126/science.1177486

10.1111/j.1600-0587.2012.00192.x

10.1093/nar/gkq118

10.1016/j.watres.2012.02.039

10.1007/s00253-012-4244-4

Hammer Ø., 2001, PAST: paleontological statistics software package for education and data analysis, Palaeontol Electron, 4, 1

10.1126/science.1200387

10.1890/06-0286

10.1101/gr.5969107

10.1007/s00253-012-4002-7

Kwon S., 2010, Bacterial community composition and diversity of a full‐scale integrated fixed‐film activated sludge system as investigated by pyrosequencing, J Microbiol Biotechnol, 20, 1717

10.1111/j.1462-2920.2009.02075.x

10.1186/1471-2105-9-386

10.1093/nar/gki866

10.2175/106143012X13373550427039

10.1890/08-0851.1

10.1093/nar/gkm864

10.1038/nature08821

10.1186/1471-2407-4-56

10.1128/AEM.01210-08

Sasaki K., 1994, Expression cloning of a novel alpha 1, 3‐fucosyltransferase that is involved in biosynthesis of the sialyl Lewis x carbohydrate determinants in leukocytes, J Biol Chem, 269, 14730, 10.1016/S0021-9258(17)36686-3

10.1128/AEM.63.7.2884-2896.1997

10.1038/ismej.2011.24

10.1126/science.1093857

10.1016/S0958-1669(02)00315-4

10.1021/es101554m

Yiannakopoulou T. Kaimakamidou V. Kungolos A. Emmanouil C. andKaragiannidis A.(2009)Testing the reliability of protozoa as indicators of wastewater treatment plant performance. InProceedings of the First International Conference on Environmental Management Engineering Planning and Economics (CEMEPE) Skiathos island Greece 24–28 June 2007: Parlar Scientific Publications pp.146–157.

10.1038/ismej.2011.188

Thông tin
Thông tin xuất bản

Environmental Microbiology Reports

Tập 6 Số 1

80-89

Thông tin tác giả