Metabonomics-based analysis of Brachyspira pilosicoli 's response to tiamulin reveals metabolic activity despite significant growth inhibition

Stanton, 1997, Physiology of Ruminal and intestinal spirochaetes, Cab. Int., 7 Duhamel, 2003, Colonic spirochetosis of colony-raised rhesus macaques associated with Brachyspira and helicobacter, Anaerobe, 9, 45, 10.1016/S1075-9964(03)00044-1 Hidalgo, 2010, Prevalence of Brachyspira pilosicoli and ‘Brachyspira canis’ in dogs and their association with diarrhoea, Vet. Microbiol., 146, 356, 10.1016/j.vetmic.2010.05.016 Trott, 1996, Serpulina pilosicoli sp., the agent of porcine intestinal spirochetosis, Int. J. Syst. Bacteriol., 46, 206, 10.1099/00207713-46-1-206 Naresh, 2010, Attraction of Brachyspira pilosicoli to mucin, Microbiology, 156, 191, 10.1099/mic.0.030262-0 Hopwood, 2007, Increasing the viscosity of the intestinal contents stimulates proliferation of enterotoxigenic Escherichia coli and Brachyspira pilosicoli in weaner pigs, Br. J. Nutr., 88, 523, 10.1079/BJN2002694 Prapasarakul, 2011, Faecal excretion of intestinal spirochaetes by urban dogs, and their pathogenicity in a chick model of intestinal spirochaetosis, Res. Vet. Sci., 91, e38, 10.1016/j.rvsc.2011.01.015 Li, 2000, Spirochete periplasmic flagella and motility, J. Mol. Microbiol. Biotechnol., 2, 345 Dworkin, 2006, The prokaryotes: vol. 7: proteobacteria: Delta and epsilon subclasses Mappley, 2012, Comparative genomics of Brachyspira pilosicoli strains: genome rearrangements, reductions and correlation of genetic compliment with phenotypic diversity, BMC Genom., 13, 454, 10.1186/1471-2164-13-454 Dassanayake, 2004, Biochemical properties of membrane-associated proteases of Brachyspira pilosicoli isolated from humans with intestinal disorders, J. Med. Microbiol., 53, 319, 10.1099/jmm.0.45549-0 Nakamura, 2006, Improvement in motion efficiency of the spirochete, Brachyspira Pilosicoli. Biophys. J., 90, 3019, 10.1529/biophysj.105.074336 Fellström, 1995, Phenotypical characterisation of intestinal spirochaetes isolated from pigs, Res. Vet. Sci., 59, 1, 10.1016/0034-5288(95)90021-7 Duhamel, 1996, Intestinal spirochaetosis and giardasis in a beagle pup with diarrhoea, Vet. Pathol., 33, 360, 10.1177/030098589603300318 Taylor, 1980, Production of diarrhoea and dysentery in pigs by feeding pure cultures of spirochaete differing from Treponemu hyodysenteriae, Vet, 106, 326 Johnston, 2001, An investigation of the etiology of a mild diarrhea observed in a group of grower/finisher pigs, Can. Vet. J., 42, 33 Wilberts, 2014, Cessation of clinical disease and spirochete shedding after tiamulin treatment in pigs experimentally infected with ‘Brachyspira hampsonii’, Res. Vet. Sci., 97, 341, 10.1016/j.rvsc.2014.08.004 Burch, 2008, Tiamulin activity against Brachyspira hyodysenteriae, Vet. Rec., 163, 698 Burch, 2013, Treatment of Brachyspira species with high MICs against Tiamulin in layers, 6th Int. Conf. Avian Intest. Spyrochaetosis Infect. Anim. Humans, 51 Stephens, 2002, Evaluation of tiamulin and lincomycin for the treatment of broiler breeders experimentally infected with the intestinal spirochaete Brachyspira pilosicoli, Avian Pathol., 31, 299, 10.1080/03079450220136501 Burch, 2006, Treatment of a field case of avian intestinal spirochaetosis caused by Brachyspira pilosicoli with tiamulin, Avian Pathol., 35, 211, 10.1080/03079450600711011 Schlünzen, 2004, Inhibition of peptide bond formation by pleuromutilins: the structure of the 50S ribosomal subunit from Deinococcus radiodurans in complex with tiamulin, Mol. Microbiol., 54, 1287, 10.1111/j.1365-2958.2004.04346.x Poulsen, 2001, The pleuromutilin drugs tiamulin and valnemulin bind to the RNA at the peptidyl transferase centre on the ribosome, Mol. Microbiol., 41, 1091, 10.1046/j.1365-2958.2001.02595.x Long, 2006, Interaction of pleuromutilin derivatives with the ribosomal peptidyl transferase center, Antimicrob. Agents Chemother., 50, 1458, 10.1128/AAC.50.4.1458-1462.2006 Forschungsinstitut, 1974, The Mode of action of pleuromutilin derivatives effect on cell-free polypeptide synthesis, Eur. J. Biochem., 533, 527 Stephens, 2002, Evaluation of tiamulin and lincomycin for the treatment of broiler breeders experimentally infected with the intestinal spirochaete Brachyspira pilosicoli, Avian Pathol., 31, 299, 10.1080/03079450220136501 Burch, 2006, Treatment of a field case of avian intestinal spirochaetosis caused by Brachyspira pilosicoli with tiamulin, Avian Pathol., 35, 211, 10.1080/03079450600711011 Sperling, 2011, Characterisation of multiresistant Brachyspira hyodysenteriae isolates from Czech pig farms, Vet. Rec., 168, 215, 10.1136/vr.c4247 Karlsson, 2004, Further characterization of porcine Brachyspira hyodysenteriae isolates with decreased susceptibility to tiamulin, J. Med. Microbiol., 53, 281, 10.1099/jmm.0.05395-0 Nicholson, 1999, ‘Metabonomics’: understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data, Xenobiotica, 29, 1181, 10.1080/004982599238047 Mappley, 2011, Lactobacilli antagonize the growth, motility, and adherence of Brachyspira pilosicoli: a potential intervention against avian intestinal spirochetosis, Appl. Environ. Microbiol., 77, 5402, 10.1128/AEM.00185-11 Gottschalk, 2012 Pringle, 2012, Antimicrobial susceptibility of porcine Brachyspira hyodysenteriae and Brachyspira pilosicoli isolated in Sweden between 1990 and 2010, Acta Vet. Scand., 54, 54, 10.1186/1751-0147-54-54 Bock, 1982, Tiamulin resistance mutations in Escherichia coli, J. Bacteriol., 151, 1253, 10.1128/JB.151.3.1253-1260.1982 Karlsson, 2001, Susceptibility to pleuromutilins in Brachyspira (serpulina) hyodysenteriae, Anim. Heal. Res. Rev., 2, 59, 10.1079/AHRR200118 Woodward, 2015, Drinking water application of Denagard® Tiamulin for control of Brachyspira pilosicoli infection of laying poultry, Res. Vet. Sci., 103, 87, 10.1016/j.rvsc.2015.09.015 Rohde, 2004, Comparison of methods for antimicrobial susceptibility testing and MIC values for pleuromutilin drugs for Brachyspira hyodysenteriae isolated in Germany, Vet. Microbiol., 102, 25, 10.1016/j.vetmic.2004.03.023