Metabolism of pyrene through phthalic acid pathway by enriched bacterial consortium composed of Pseudomonas, Burkholderia, and Rhodococcus (PBR)

3 Biotech - Tập 7 - Trang 1-15 - 2017
Sagar Vaidya1, Kunal Jain1, Datta Madamwar1
1Environmental Genomics and Proteomics Lab, UGC Centre of Advanced Study, P. G. Department of Biosciences, Sardar Patel University, Anand, India

Tóm tắt

Polycyclic aromatic hydrocarbons (PAHs) are highly recalcitrant compounds due to their high hydrophobicity and tendency to partition in organic phase of soils. Pyrene is a high-molecular weight PAH, which has human health concerns. In the present study, a bacterial consortium, PBR, was developed from a long-term polluted site, viz., Amlakhadi, Ankleshwar, Gujarat, for effective degradation of pyrene. The consortium effectively metabolized pyrene as a sole source of carbon and energy. The consortium comprised three bacterial species, Pseudomonas sp. ASDP1, Burkholderia sp. ASDP2, and Rhodococcus sp. ASDP3. The maximum growth rate of consortium was 0.060/h and the maximum pyrene degradation rate was 16 mg/l/day. The organic and inorganic nutrients along with different surfactants did not affect pyrene degradation, but degradation rate moderately increased in the presence of sodium succinate. The significant characteristic of the consortium was that it possessed an ability to degrade six other hydrocarbons, both independently and simultaneously at 37 °C, in BHM (pH 7.0) under shaking conditions (150 rpm) and it showed resistance towards mercury at 10 mM concentration. Phthalic acid as one of the intermediates during pyrene degradation was detected through high-performance liquid chromatography (HPLC). The efficiency of consortium for pyrene degradation was validated in simulated microcosms’ study, which indicated that 99% of pyrene was metabolized by the consortium under ambient conditions.

Tài liệu tham khảo

Abdelhay A, Magnin JP, Gondrexon N, Baup S, Willison J (2008) Optimization and modeling of phenanthrene degradation by Mycobacterium sp. 6PY1 in a biphasic medium using response-surface methodology. Appl Microbiol Biotechnol 78:881–888 Ascon-Cabrera M, Lebeault JM (1993) Selection of xenobiotic-degrading microorganisms in a biphasic aqueous–organic system. Appl Environ Microbiol 59:1717–1724 Atlas RM, Hazen TC (2011) Oil Biodegradation and Bioremediation: a tale of the Two Worst Spills in US History. Environ Sci Technol 45(16):6709–6715 Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JA, Smith JG, Struhl DJ (1997) Current protocols in molecular biology. Wiley, New York (Unit 24) Bacosa and Inoue (2015) Polycyclic aromatic hydrocarbons (PAHs) biodegradation potential and diversity of microbial consortia enriched from tsunami sediments in Miyagi. J Hazard Mater 283(2015):689–697 Bacosa HP, Suto K, Inoue C (2013) Degradation potential and microbial community structure of heavy oil-enriched microbial consortia from mangrove sediments in Okinawa, Japan. J Environ Sci Health A 48:1–12 Boopathy R (2000) Factors limiting bioremediation technologies. Bioresour Technol 74:63–67 Boyd TJ, Montgomery MT, Steele JK, Pohlman JW, Reatherford SR, Spargo BJ, Smith DC (2005) Dissolved oxygen saturation controls PAH biodegradation in freshwater estuary sediments. Microbiol Ecol 49:1–10 Chen Y, Cheng JJ, Creamer KS (2008) Inhibition of anaerobic digestion process: a review. Bioresour Technol 99:4044–4064 Desai C, Madamwar D (2007) Extraction of inhibitor-free metagenomic DNA from polluted sediments, compatible with molecular diversity analysis using adsorption and ion-exchange treatments. Bioresour Technol 98(4):761–768 Dibble JT, Bartha R (1979) Effect f environmental parameters on the biodegradation of oil sludge. Appl Environ Microbiol 37:729–739 Doick KJ, Klingelmann E, Burauel P, Jones KC, Semple KT (2005) Long term fate of polychlorinated biphenyls and polycyclic aromatic hydrocarbons in agricultural soil. Environ Sci Techn 39:3663–3670 Ghosh I, Jasmin J, Mukherji S (2014) Biodegradation of pyrene by Pseudomonas aeruginosa strain RS1 isolated from refinery sludge. Bioresour Technol 166:548–558 Hambrick GA, DeLaune RD, Patrick WH Jr (1980) Effect of estuarine sediment pH and oxidation-reduction potential on microbial hydrocarbon degradation. Appl Environ Microbiol 40:365–369 Hamzah A, Tavakoli A, Rabu A (2011) Detection of toluene degradation in bacteria isolated from oil contaminated soils. Sains Malays 40(11):1231–1235 Haritash AK, Kaushik CP (2009) Biodegradation aspects of Polycyclic Aromatic Hydrocarbons (PAHs): a review. J Hazard Mater 169:1–15 Ho Y, Jackson M, Yang Y, Mueller JG, Pritchard PH (2000) Characterization of fluoranthene- and pyrene-degrading bacteria isolated from PAH-contaminated soils and sediments and comparison of several Sphingomonas spp. J Ind Microbiol 2:100–112 Hughes MN, Poole RK (1991) Metal speciation and microbial growth—the hard (and soft) facts. J Gen Microbiol 137:725–734 Jacques RJS, Santos EC, Bento FM, Peralba MCR, Selbach PA, Sá ELS, Camargo FAO (2005) Anthracene biodegradation by Pseudomonas sp. isolated from a petrochemical sludge land farming site. Int Biodeterior Biodegrad 56:143–150 Johnson AR, Wick LY, Harms H (2005) Principles of microbial PAH-degradation in soil. Environ Pollut 133:71–84 Juhasz AL, Naidu R (2000) Bioremediation of high molecular weight polycyclic aromatic hydrocarbons: a review of the microbial degradation of benzo[α]pyrene. Inter Biodeter Biodegrad 45:57–88 Kathuria V (2007) Informal regulation of pollution in a developing country: evidence from India. Ecolo Econ 63:403–417 Krishnan S, Prabhu Y, Phale PS (2004) o-Phthalic acid, a dead end product in one of two pathways of phenanthrene degradation in Pseudomonas sp. strain PP2. Indian J Biochem Biophys 41:227–232 Kumar A, Munjal A, Sawhney R (2011) Crude oil PAH constitution, degradation pathway and associated bioremediation microflora: an overview. Inter J Environ Sci 1(7):1420–1439 Leahy JL, Colwell RR (1990) Microbial degradation of hydrocarbons in the environment. Microbiol Rev 54:305–315 Mrozik A, Piotrowska-Seget Z, Labuzak S (2003) Bacterial degradation and bioremediation of polycyclic aromatic hydrocarbons. Polish J Environ Stud 12(1):15–25 Mukherjee P, Roy P (2013) Copper enhanced monooxygenase activity and FT-IR spectroscopic characterisation of biotransformation products in trichloroethylene degrading bacterium: Stenotrophomonas maltophilia PM102. BioMed Res Int 2013:1–9 Article ID 723680 Okpokwasili GC, Nweke CO (2005) Microbial growth and substrate utilization kinetics. Afr J Biotechnol 5:305–317 Patel V, Madamwar D (2013) Biodegradation of phenanthrene in bioaugmented microcosm by consortium ASP developed using coastal sediment of Alang–Sosiya ship breaking yard, Gujarat. Mar Pollut Bull 74:199–207 Patel V, Cheturvedula S, Madamwar D (2012a) Phenanthrene degradation by Pseudoxanthomonas sp. DMVP2 isolated from hydrocarbon contaminated sediment of Amlakhadi canal, Gujarat, India. J Hazard Mater 201–202:43–51 Patel V, Jain S, Madamwar D (2012b) Naphthalene degradation by bacterial consortium (DV-AL) developed from Alang–Sosiya ship breaking yard, Gujarat, India. Bioresour Technol 107:122–130 Pathak H, Kantharia D, Malpani A, Madamwar D (2009) Naphthalene degradation by Pseudomonas sp. HOB1: in vitro studies and assessment of naphthalene degradation efficiency in simulated microcosms. J Hazard Mater 166:1466–1473 Pathak H, Patel S, Rathod M, Chauhan K (2011) In vitro studies on degradation of synthetic dye mixture by Comamonas sp. VS-MH2 and evaluation of its efficacy using simulated microcosm. Bioresour Technol 102:10391–10400 Patrick WH Jr, DeLaune RD (1977) Chemical and biological redox systems affecting nutrient availability in coastal wetlands. Geosci Man 18:131–137 Pepi M, Heipieper HJ, Fischer J, Ruta M, Volterrani M, Focardi SE (2008) Membrane fatty acids adaptive profile in the simultaneous presence of arsenic and toluene in Bacillus sp. ORAs2 and Pseudomonas sp. ORAs5 strains. Extremophiles 12:343–349 Ravanipour M, Kalantary RR, Mohseni-Bandpi A, Esrafili A, Farzadkia M, Hashemi-Najafabadi S (2015) Experimental design approach to the optimization of PAHs bioremediation from artificially contaminated soil: application of variables screening development. J Environ Heal Sci Eng 13:22 Sandrin TR, Chech AM, Maier RM (2000) A Rhamnolipid biosurfactant reduces cadmium toxicity during naphthalene biodegradation. Appl Environ Microbiol 66:4585–4588 Seo J, Keum Y, Li QX (2009) Bacterial degradation of aromatic compounds. Inter J Environ Res Public Health 6:278–309 Shuttleworth KL, Cerniglia CE (1995) Environmental aspects of PAH biodegradation. Appl Biochem Biotechnol 54:291–302 Verstraete WR, Vanloocke R, De Borger, Verlinde A (1976) Modelling of the breakdown and the mobilization of hydrocarbons in unsaturated soil layers. In: Sharpley JM, Kaplan AM (ed) Proceedings of the 3rd international biodegradation symposium. Applied Science Publishers Ltd., London, pp 99–112 Walter U, Beyer M, Klein J, Rehm HJ (1991) Degradation of pyrene by Rhodococcus UW1. Appl Microbiol Biotechnol 34:671–676 Wang BJ, Lai QL, Cui ZS, Tan TF, Shao ZZ (2008) A pyrene-degrading consortium from deep-sea sediment of the West Pacific and its key member Cycloclasticus sp P1. Environ Microbiol 10(8):1948–1963 Yuan SY, Shiung LC, Chang BV (2002) Biodegradation of polycyclic aromatic hydrocarbons by inoculated microorganisms in soil. Bull Environ Contam Toxicol 69:66–73