Metabolic stress activates an ERK/hnRNPK/DDX3X pathway in pancreatic β cells

Prentki, 2006, Islet β cell failure in type 2 diabetes, Journal of Clinical Investigation, 116, 1802, 10.1172/JCI29103 Talchai, 2012, Pancreatic β cell dedifferentiation as a mechanism of diabetic β cell failure, Cell, 150, 1223, 10.1016/j.cell.2012.07.029 Kim-Muller, 2016, Aldehyde dehydrogenase 1a3 defines a subset of failing pancreatic β cells in diabetic mice, Nature Communications, 7, 1, 10.1038/ncomms12631 Mitchell, 2014, Principles and properties of eukaryotic mRNPs, Molecular Cell, 54, 547, 10.1016/j.molcel.2014.04.033 Uniacke, 2012, An oxygen-regulated switch in the protein synthesis machinery, Nature, 486, 126, 10.1038/nature11055 Harvey, 2017, Post-transcriptional control of gene expression following stress: the role of RNA-binding proteins, Biochemical Society Transactions, 45, 1007, 10.1042/BST20160364 Knoch, 2004, Polypyrimidine tract-binding protein promotes insulin secretory granule biogenesis, Nature Cell Biology, 6, 207, 10.1038/ncb1099 Magro, 2013, Regulation of β-cell function by RNA-binding proteins, Molecular Metabolism, 2, 348, 10.1016/j.molmet.2013.09.003 Bomsztyk, 2004, hnRNP K: one protein multiple processes, BioEssays, 26, 629, 10.1002/bies.20048 Habelhah, 2001, ERK phosphorylation drives cytoplasmic accumulation of hnRNP-K and inhibition of mRNA translation, Nature Cell Biology, 3, 325, 10.1038/35060131 Ostrowski, 2001, Insulin alters heterogeneous nuclear ribonucleoprotein K protein binding to DNA and RNA, Proceedings of the National Academy of Sciences, 98, 9044, 10.1073/pnas.161284098 Ostareck-Lederer, 2002, c-Src-mediated phosphorylation of hnRNP K drives translational activation of specifically silenced mRNAs, Molecular and Cellular Biology, 22, 4535, 10.1128/MCB.22.13.4535-4543.2002 Hutchins, 2013, c-Jun N-terminal kinase phosphorylation of heterogeneous nuclear ribonucleoprotein K regulates vertebrate axon outgrowth via a posttranscriptional mechanism, Journal of Neuroscience, 33, 14666, 10.1523/JNEUROSCI.4821-12.2013 Good, 2019, JUND regulates pancreatic β cell survival during metabolic stress, Molecular Metabolism, 25, 95, 10.1016/j.molmet.2019.04.007 Sastry, 2002, Titering lentiviral vectors: comparison of DNA, RNA and marker expression methods, Gene Therapy, 9, 1155, 10.1038/sj.gt.3301731 Claiborn, 2010, Pcif1 modulates Pdx1 protein stability and pancreatic β cell function and survival in mice, Journal of Clinical Investigation, 120, 3713, 10.1172/JCI40440 Morgenstern, 1990, Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line, Nucleic Acids Research, 18, 3587, 10.1093/nar/18.12.3587 Doyle, 2008, Application of a translational profiling approach for the comparative analysis of CNS cell types, Cell, 135, 749, 10.1016/j.cell.2008.10.029 Kinoshita, 2006, Phosphate-binding tag, a new tool to visualize phosphorylated proteins, Molecular & Cellular Proteomics – MCP, 5, 749, 10.1074/mcp.T500024-MCP200 Sanjana, 2014, Improved vectors and genome-wide libraries for CRISPR screening, Nature Methods, 11, 783, 10.1038/nmeth.3047 Campeau, 2009, A versatile viral system for expression and depletion of proteins in mammalian cells, PLoS One, 4, e6529, 10.1371/journal.pone.0006529 Kim, 2013, TopHat2: accurate alignment of transcriptomes in the presence of insertions, deletions and gene fusions, Genome Biology, 14, R36, 10.1186/gb-2013-14-4-r36 Liao, 2014, featureCounts: an efficient general purpose program for assigning sequence reads to genomic features, Bioinformatics, 30, 923, 10.1093/bioinformatics/btt656 Bailey, 2009, MEME SUITE: tools for motif discovery and searching, Nucleic Acids Research, 37, W202, 10.1093/nar/gkp335 Ray, 2014, A compendium of RNA-binding motifs for decoding gene regulation, Nature, 499, 172, 10.1038/nature12311 Stoffers, 1997, Early-onset type-ll diabetes mellitus (MODY4) linked to IPF1, Nature Genetics, 17, 138, 10.1038/ng1097-138 Sachdeva, 2009, Pdx1 (MODY4) regulates pancreatic beta cell susceptibility to ER stress, Proceedings of the National Academy of Sciences of the United States of America, 106, 19090, 10.1073/pnas.0904849106 Bailey, 1994, Fitting a mixture model by expectation maximization to discover motifs in biopolymers, Proceedings International Conference on Intelligent Systems for Molecular Biology, 2, 28 The ENCODE Project Consortium, 2012, An integrated encyclopedia of DNA elements in the human genome, Nature, 488, 57, 10.1038/nature11247 Kimura, 2010, Characterization of multiple alternative forms of heterogeneous nuclear ribonucleoprotein K by phosphate-affinity electrophoresis, Proteomics, 10, 3884, 10.1002/pmic.201000349 Marroqui, 2015, Pancreatic α cells are resistant to metabolic stress-induced apoptosis in type 2 diabetes, EBioMedicine, 2, 378, 10.1016/j.ebiom.2015.03.012 Lubelsky, 2018, Sequences enriched in Alu repeats drive nuclear localization of long RNAs in human cells, Nature, 555, 107, 10.1038/nature25757 Short, 2002, Translational regulation of the JunD messenger RNA, Journal of Biological Chemistry, 277, 32697, 10.1074/jbc.M204553200 Hartman, 2006, RNA helicase A is necessary for translation of selected messenger RNAs, Nature Structural & Molecular Biology, 13, 509, 10.1038/nsmb1092 Mikula, 2015, Beads-free protein immunoprecipitation for a mass spectrometry-based interactome and posttranslational modifications analysis, Proteome Science, 1 Chen, 2002, An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K, Journal of Biological Chemistry, 277, 40403, 10.1074/jbc.M206981200 Linder, 2011, From unwinding to clamping - the DEAD box RNA helicase family, Nature Publishing Group, 12, 505 Soto-Rifo, 2012, DEAD-box protein DDX3 associates with eIF4F to promote translation of selected mRNAs, The EMBO Journal, 31, 3745, 10.1038/emboj.2012.220 Oh, 2016, Medulloblastoma-associated DDX3 variant selectively alters the translational response to stress, Oncotarget, 7, 28169, 10.18632/oncotarget.8612 Guenther, 2018, The helicase Ded1p controls use of near-cognate translation initiation codons in 5′ UTRs, Nature, 1 Çelik, 2015, Ezrin binds to DEAD-box RNA helicase DDX3 and regulates its function and protein level, Molecular and Cellular Biology, 107, 15 Bolger, 2011, Gle1 is a multifunctional DEAD-box protein regulator that modulates Ded1 in translation initiation, Journal of Biological Chemistry, 286, 39750, 10.1074/jbc.M111.299321 Schullery, 1999, Regulated interaction of protein kinase Cdelta with the heterogeneous nuclear ribonucleoprotein K protein, Journal of Biological Chemistry, 274, 15101, 10.1074/jbc.274.21.15101 Maedler, 2004, Diabetes, 53, 1706, 10.2337/diabetes.53.7.1706 Quoyer, 2010, GLP-1 mediates antiapoptotic effect by phosphorylating Bad through a beta-arrestin 1-mediated ERK1/2 activation in pancreatic beta-cells, Journal of Biological Chemistry, 285, 1989, 10.1074/jbc.M109.067207 Campbell, 2016, TCF1 links GIPR signaling to the control of beta cell function and survival, Nature Medicine, 22, 84, 10.1038/nm.3997 Banks, 2015, An ERK/Cdk5 axis controls the diabetogenic actions of PPARγ, Nature, 517, 391, 10.1038/nature13887 Ariumi, 2014, Multiple functions of DDX3 RNA helicase in gene regulation, tumorigenesis, and viral infection, Frontiers in Genetics, 5, 423