Metabolic engineering of Saccharomyces cerevisiae for hydroxytyrosol overproduction directly from glucose

Microbial Biotechnology - Tập 15 Số 5 - Trang 1499-1510 - 2022
Ricardo Bisquert1, Andrés Planells‐Cárcel1, Elena Valera‐García1, José Manuel Guillamón1, Sara Muñiz‐Calvo1
1Departamento de Biotecnología de Alimentos Instituto de Agroquímica y Tecnología de Alimentos IATA‐CSIC Agustín Escardino 7 Paterna Valencia 46980 Spain

Tóm tắt

SummaryHydroxytyrosol (HT) is one of the most powerful dietary antioxidants with numerous applications in different areas, including cosmetics, nutraceuticals and food. In the present work, heterologous hydroxylase complex HpaBC from Escherichia coli was integrated into the Saccharomyces cerevisiae genome in multiple copies. HT productivity was increased by redirecting the metabolic flux towards tyrosol synthesis to avoid exogenous tyrosol or tyrosine supplementation. After evaluating the potential of our selected strain as an HT producer from glucose, we adjusted the medium composition for HT production. The combination of the selected modifications in our engineered strain, combined with culture conditions optimization, resulted in a titre of approximately 375 mg l−1 of HT obtained from shake‐flask fermentation using a minimal synthetic‐defined medium with 160 g l−1 glucose as the sole carbon source. To the best of our knowledge, this is the highest HT concentration produced by an engineered S. cerevisiae strain.

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Microbial Biotechnology

Tập 15 Số 5

1499-1510

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