Metabolic diversity of Saccharomyces cerevisiae strains from spontaneously fermented grape musts

World Journal of Microbiology and Biotechnology - Tập 19 - Trang 311-315 - 2003
Patrizia Romano1, Marisa Caruso1, Angela Capece1, Gabriella Lipani1, Margherita Paraggio1, Concetta Fiore1
1Dipartimento di Biologia, Difesa e Biotecnologie Agroforestali, Università degli Studi della Basilicata, Campus Macchia Romana, Potenza, Italy; Tel.:

Tóm tắt

One hundred and fifteen Saccharomyces cerevisiae strains from Aglianico del Vulture, a red wine produced in Southern Italy, were characterized for the production of some secondary compounds involved in the aroma and taste of alcoholic beverages. The strains exhibited a uniform behaviour in the production levels of n-propanol, active amyl alcohol and ethyl acetate, whereas isobutanol, isoamyl alcohol and acetaldehyde were formed with a wide variability. Only five strains produced wines close to the reference Aglianico del Vulture wine for the traits considered. Of these, two strains were selected, underwent to tetrad analysis and the single spore cultures were tested in grape must fermentation. The progeny of one strain showed a significant metabolic variability, confirming the necessity to test starter cultures for the segregation of traits of technological interest. Our findings suggest the selection of specific strains for specific fermentations as a function of the vine variety characteristics in order to take the major advantage from the combination grape must/S. cerevisiae strain.

Tài liệu tham khảo

Cabrera, M.J., Moreno, J., Ortega, J.M. & Medina, M. 1988 Formation of ethanol, higher alcohols, esters and terpenes by five yeast strains in musts from Pedro Ximenez grapes in various degrees of ripeness. American Journal of Enology and Viticulture 39, 283–287. Frezier, V.D. & Dubourdieu, D. 1992 Ecology of yeast strain Saccharomyces cerevisiae during spontaneous fermentation in a Bordeaux winery. American Journal of Enology and Viticulture 43, 375–380. Giudici, P., Romano, P. & Zambonelli, C. 1990 A biometric study of higher alcohol production in Saccharomyces cerevisiae. Canadian Journal of Microbiology 36, 61–64. Henschke, P.A. 1997 Wine yeast. In Yeast Sugar Metabolism: Biochemistry, Genetics, Biotechnology and Applications, eds. Zimmermann, F.K. & Entian, K.D. pp. 527–560. Lancaster PA: Technomic. ISBN 1-56676466-1. Kurtzman, C.P. & Fell, J.W. 1998 The Yeasts-A Taxonomic Study. p. 1055. The Netherlands: Elsevier. ISBN 0-44481312-8. Lema, C., Garcia-Jares, C., Orriols, I. & Angulo, L. 1996 Contribution of Saccharomyces and non-Saccharomyces populations to the production of some components of Albariö wine aroma. American Journal of Enology and Viticulture 47, 206–216. Lurton, L., Snakkers, G., Roulland, C., Galy, B. & Versavaud, A. 1995 Influence of the fermentation yeast strain on the composition of wine spirits. Journal of Science of Food and Agriculture 67, 485–491. Mortimer, R. & Polsinelli, M. 1999 On the origins of wine yeast. Research in Microbiology 150, 199–204. Mortimer, R., Romano, P., Suzzi, G. & Polsinelli, M. 1994 Genome renewal: a new phenomenon revealed from a genetic study of 43 strains of Saccharomyces cerevisiae derived from natural fermentation of grape musts. Yeast 10, 1543–1552. Polsinelli, M., Romano, P., Suzzi, G. & Mortimer, M. 1996 Multiple strains of Saccharomyces cerevisiae on a single grape wine. Letters in Applied Microbiology 22, 110–114. Pretorius, I.S. 2000 Tailoring wine yeast for the new millennium: novel approaches to the ancient art of winemaking. Yeast 16, 675–729. Querol, A., Barrio, E. & Ramon, D. 1992 A comparative study of different methods of yeast strain characterization. Systematic and Applied Microbiology 15, 439–446. Rankine, B.C. 1968 The importance of yeasts in determining the composition and quality of wines. Vitis 7, 22–49. Romano, P. 1990 La purezza fermentativa in Saccharomyces cerevisiae. Industrie delle Bevande 19, 320–321. Romano, P. 1998 Metabolic characteristics of wine strains during spontaneous and inoculated fermentation. Food Technology and Biotechnology 35, 255–260. Romano, P. & Suzzi, G. 1993 Acetoin production by Saccharomyces cerevisiae wine yeasts. FEMS Microbiology Letters 108, 23–26. Romano, P., Monteleone, E., Paraggio, M., Marchese, R. & Caporale, G. 1998a A methodological approach for the selection of Saccharomyces cerevisiae wine strains. Food Technology and Biotechnology 36, 69–74. Romano, P., Paraggio, M. & Turbanti, L. 1998b Stability in byproduct formation as a strain selection tool of Saccharomyces cerevisiae wine yeasts. Journal of Applied Microbiology 84, 336–341. Romano, P., Suzzi, G., Turbanti, L. & Polsinelli, M. 1994 Acetaldehyde production in Saccharomyces cerevisiae wine yeasts. FEMS Microbiology Letters 118, 213–218. Schütz, M. & Gafner, J. 1993 Analysis of yeast diversity during spontaneous and induced alcoholic fermentation. Journal of Applied Bacteriology 75, 551–558. Soufleros, E. & Bertrand, A. 1979 Role de 'souche de levure' dans la production des substances volatiles en cours de la fermenation du jus de raisin. Connaissance de la Vigne et du Vin 13, 181–198. Vaughan-Martini, A. & Martini, A. 1998 Determination of ethanol production. In The Yeasts. A Taxonomic Study, eds. Kurtzman, C.P. & Fell, J.V. p. 107. The Netherlands: Elsevier. ISBN 0-44481312-8.