Metabolic changes by acute insulin deficiency in rabbits injected with anti-insulin serum

Acute insulin deficiency was produced in rabbits treated with anti-insulin guinea pig serum. The changes in the levels of blood sugar, free fatty acids, aceto-acetate, SGOT, SGPT, liver glutamicoxaloacetic and glutamic-pyruvic transaminases, and liver lactate dehydrogenase were examined. A significant increase of the blood glucose level and the free fatty acids as well as glycosuria was observed. The other examined parameters remained unchanged. The results confirm the statement that acute insulin deficiency causes a prompt impairment of glucose and fat metabolism.