Metabolic-Flux Profiling of the Yeasts Saccharomyces cerevisiae and Pichia stipitis

American Society for Microbiology - Tập 2 Số 1 - Trang 170-180 - 2003
Jocelyne Fiaux1, Zeynep Petek Çakar2, Marco Sonderegger2, Kurt Wüthrich1, Thomas Szyperski3, Uwe Sauer2
1Institute of Molecular Biology and Biophysics
2Institute of Biotechnology, ETH Zürich, CH-8093 Zürich, Switzerland
3Department of Chemistry, State University of New York, Buffalo, New York 14260

Tóm tắt

ABSTRACT The so far largely uncharacterized central carbon metabolism of the yeast Pichia stipitis was explored in batch and glucose-limited chemostat cultures using metabolic-flux ratio analysis by nuclear magnetic resonance. The concomitantly characterized network of active metabolic pathways was compared to those identified in Saccharomyces cerevisiae , which led to the following conclusions. (i) There is a remarkably low use of the non-oxidative pentose phosphate (PP) pathway for glucose catabolism in S. cerevisiae when compared to P. stipitis batch cultures. (ii) Metabolism of P. stipitis batch cultures is fully respirative, which contrasts with the predominantly respiro-fermentative metabolic state of S. cerevisiae . (iii) Glucose catabolism in chemostat cultures of both yeasts is primarily oxidative. (iv) In both yeasts there is significant in vivo malic enzyme activity during growth on glucose. (v) The amino acid biosynthesis pathways are identical in both yeasts. The present investigation thus demonstrates the power of metabolic-flux ratio analysis for comparative profiling of central carbon metabolism in lower eukaryotes. Although not used for glucose catabolism in batch culture, we demonstrate that the PP pathway in S. cerevisiae has a generally high catabolic capacity by overexpressing the Escherichia coli transhydrogenase UdhA in phosphoglucose isomerase-deficient S. cerevisiae .

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American Society for Microbiology

Tập 2 Số 1

170-180

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