Melatonin improves placental efficiency and birth weight and increases the placental expression of antioxidant enzymes in undernourished pregnancy

Journal of Pineal Research - Tập 46 Số 4 - Trang 357-364 - 2009
Hans G. Richter1, Jeremy A. Hansell1, Shruti Raut2, Dino A. Giussani2
1these authors contributed equally to this work
2Centre for Trophoblast Research, Department of Physiology, Development & Neuroscience, University of Cambridge, Cambridge, UK

Tóm tắt

Abstract:  Melatonin participates in circadian, seasonal and reproductive physiology. Melatonin also acts as a potent endogenous antioxidant by scavenging free radicals and upregulating antioxidant pathways. The placenta expresses melatonin receptors and melatonin protects against oxidative damage induced in rat placenta by ischemia‐reperfusion. One of the most common complications in pregnancy is a reduction in fetal nutrient delivery, which is known to promote oxidative stress. However, whether melatonin protects placental function and fetal development in undernourished pregnancy is unknown. Here, we investigated the effects of maternal treatment with melatonin on placental efficiency, fetal growth, birth weight and protein expression of placental oxidative stress markers in undernourished pregnancy. On day 15 of pregnancy, rats were divided into control and undernourished pregnancy (35% reduction in food intake), with and without melatonin treatment (5 μg/mL drinking water). On day 20 of gestation, fetal biometry was carried out, the placenta was weighed and subsequently analyzed by Western blot for xanthine oxidase, heat shock protein (HSP) 27 and 70, catalase, manganese superoxide dismutase (Mn‐SOD) and glutathione peroxidase 1 (GPx‐1). A separate cohort was allowed to deliver to assess effects on birth weight. Maternal undernutrition led to a fall in placental efficiency, disproportionate intrauterine growth retardation and a reduction in birth weight. Maternal treatment with melatonin in undernourished pregnancy improved placental efficiency and restored birth weight, and it increased the expression of placental Mn‐SOD and catalase. The data show that in pregnancy complicated by undernutrition, melatonin may improve placental efficiency and birth weight by upregulating placental antioxidant enzymes.

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Tài liệu tham khảo

10.1530/acta.0.1240233

10.3109/00207459108999775

10.1177/074873049701200605

10.1034/j.1600-079X.2001.300104.x

10.1016/j.reprotox.2008.03.005

10.1016/S0303-7207(02)00414-8

10.1111/j.1600-079X.2005.00244.x

10.1111/j.1600-079X.2008.00555.x

10.1385/CBB:34:2:237

10.1385/ENDO:27:2:119

10.1007/s00018-008-8001-x

10.18388/abp.2003_3637

10.1096/fasebj.10.8.8666165

10.1016/S0891-5849(99)00131-8

10.1046/j.1600-079X.2003.00092.x

10.1111/j.1600-079X.2005.00248.x

10.1111/j.1600-079X.2005.00295.x

10.1111/j.1600-079X.2004.00181.x

10.1186/1743-7075-2-22

10.1111/j.1600-079X.2006.00407.x

10.1111/j.1600-079X.2008.00580.x

10.1053/plac.2002.0792

10.1093/ajcn/80.1.193

10.1136/adc.2005.082297

10.1152/physiol.00050.2005

10.1016/S0008-6363(03)00461-9

10.1016/j.lfs.2006.10.028

Halliwell B, 2004, Free Radicals in Biology and Medicine

10.1016/j.mehy.2005.08.020

10.1042/CS20070113

10.1034/j.1600-079X.2001.300402.x

10.1111/j.1600-079X.2008.00586.x

Herxheimer A, 2002, Melatonin for the prevention and treatment of jet lag, Cochrane Database Syst Rev, 2, CD001520

10.2353/ajpath.2006.060161

Zar JH, 1984, Biostatistical Analysis, 236

10.1016/S0140-6736(07)61690-0

10.1053/plac.2002.0802

10.1016/1071-5576(96)00015-9

10.1034/j.1600-079X.2001.280204.x

10.1095/biolreprod39.5.1093

10.1677/joe.0.1200459

10.1210/en.136.1.218

10.1111/j.1600-079X.2007.00537.x

10.1016/0006-8993(95)96872-I

10.1093/toxsci/50.2.271

10.1080/10623320490482655

10.2174/0929867043365369

10.1016/j.molmed.2006.03.003

10.2353/ajpath.2007.070528

10.1111/j.1600-079X.1998.tb00371.x

10.1111/j.1365-2826.2008.01663.x

10.1111/j.1600-079X.2006.00375.x

10.1111/j.1600-079X.2008.00641.x

10.1111/j.1600-079X.2007.00548.x

10.1111/j.1600-079X.2008.00608.x

10.1016/j.siny.2004.03.008

10.1111/j.1471-0528.1997.tb11975.x

10.1067/mob.2002.119629

10.1111/j.1471-0528.2007.01607.x

10.1016/0041-008X(92)90194-W

Timcenko‐Youssef L, 1985, Subcellular localization of adrenal cortical glutathione peroxidase and protective role of the mitochondrial enzyme against lipid peroxidative damage, J Biol Chem, 260, 13355, 10.1016/S0021-9258(17)38877-4

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Journal of Pineal Research

Tập 46 Số 4

357-364

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