Mediated amperometry reveals two distinct modes of yeast responses to glucose

Central European Journal of Biology - Tập 9 - Trang 173-181 - 2013
Rasa Garjonyte1, Vytautas Melvydas2, Algimantas Paškevičius2, Valerijus Rašomavičius2, Albertas Malinauskas1
1Center for Physical Sciences and Technology, Institute of Chemistry, Vilnius, Lithuania
2Nature Research Center, Institute of Botany, Vilnius, Lithuania

Tóm tắt

Mediated amperometry was exploited to monitor intracellular redox activity without cell disruption. Continuous measurements of menadione-mediated glucose currents at carbon paste electrodes with various immobilized intact wild type yeasts (Saccharomyces cerevisiae, Candida pulcherrima, Clavispora lusitaniae, Wickerhamomyces anomalus, Pichia guilliermondii, Kluyveromyces lactis var. lactis, Debaryomyces hansenii, Candida zeymolaydes and Candida tropicalis) revealed two distinct and previously unreported modes of development of the currents during the first 2 to 3 min. after subjection to glucose. A correlation among the values of the currents and the capacities of wild type yeasts to secrete various substances was observed.

Tài liệu tham khảo

Kurtzman C.P., Fell J.W., Boekhout T., The Yeasts: a Taxonomic Study, 5th ed., Elsevier, Amsterdam, 2010 Zhao J., Wang Z., Fu C., Wang M., He Q., The mediated electrochemical method for rapid fermentation ability assessment, Electroanal. 2008, 20, 1587–1592 Zhao J., Wang M., Yang Z., Gong Q., Lu Y., Yang Z., Mediated electrochemical measurement of the inhibitory effects of furfural and acetic acid on Saccharomyces cerevisiae and Candida shehatae, Biotechnol. Lett., 2005, 27, 207–211 Wang M., Zhao J., Yang Z., Du Z., Yang Z., Electrochemical insights into the ethanol tolerance of Saccharomyces cerevisiae, Bioelectrochem., 2007, 71, 107–112 Zhao J., Wang Z., Wang M., Wang H., He Q., Zhang H., The interaction mechanisms between Saccharomyces cerevisiae and menadione and its application in toxicology study, Talanta, 2008, 74, 1686–1691 Baronian K.H.R., Downard A.J., Lowen R.K., Pasco N., Detection of two distinct substrate-dependent catabolic responses in yeast cells using a mediated electrochemical method, Appl. Microbiol. Biotechnol., 2002, 60, 108–113 Heiskanen A., Yakovleva J., Spegel C., Taboryski R., Koudelka-Hep M., Emneus J., et al., Amperometric monitoring of redox activity in living yeast cells: comparison of menadione and menadione bisulfite as electron transfer mediators, Electrochem. Commun., 2004, 6, 219–224 Spegel C.F., Heiskanen A.R., Kostesha N.V., Johanson T.H., Gorwa-Grauslund M.F., Koudelka-Hep M., et al., Amperometric response from the glycolytic versus the pentose phosphate pathway in Saccharomyces cerevisiae cells, Anal. Chem., 2007, 79, 8919–8926 Zhao J., Yang Z., Gong Q., Lu Y., Yang Z., Wang M., Electrochemical insights into the glucose metabolism pathways within Saccharomyces cerevisiae, Anal. Lett., 2005, 38, 89–98 Heiskanen A., Spegel C., Kostesha N., Lindahl S., Ruzgas T., Emneus J., Mediator-assisted simultaneous probing of cytosolic and mitochondrial redox activity in living cells, Anal. Biochem., 2009, 384, 11–19 Kostesha N.V, Almeida J.R.M., Heiskanen A.R., Gorwa-Grauslund M.F., Hahn-Hagerdal B., Emneus J., Electrochemical probing in vivo 5-hydoxymethyl furfural reduction in Saccharomyces cerevisiae, Anal. Chem., 2009, 81, 9896–9901 Kostesha N., Heiskanen A., Spegel C., Hahn-Hagerdal B., Gorwa-Grauslund M.F., Emneus J., Real-time detection of cofactor availability in genetically modified living Saccharomyces cerevisiae cells -Simultaneous probing of different geno- and phenotypes, Bioelectrochem., 2009, 76, 180–188 Rolland F., Winderickx J., Thevelein J.M., Glucosesensing and -signalling mechanisms in yeast, FEMS Yeast Res., 2002, 2, 183–201 Santangelo G.M, Glucose signaling in Saccharomyces cerevisiae, Microbiol. Mol. Biol. Rev., 2006, 70 253–282 Gancedo J.M., The early steps of glucose signaling in yeast, FEMS Microbiol. Rev., 2008, 32, 673–704 Busti S., Coccetti P., Alberghina L., Vanoni M., Glucose signaling-mediated coordination of cell growth and cell cycle in Saccharomyces cerevisiae, Sensors, 2010, 10, 6195–6240 Gulbiniene G., Kondratiene L, Jokantaite T., Serviene E., Melvydas V., Petkuniene G., Occurrence of killer yeast strains in fruit and berry wine yeast populations, Food Technol. Biotechnol., 2004, 42, 159–163 Melvydas V., Serviene E., Cernishova O., Petkuniene G., A novel X factor secreted by yeasts inhibits Saccharomyces cerevisiae K1, K2 and K28 killer toxins, Biologija, 2007, 53, 32–35 Melvydas V., Serviene E., Kondratiene L., Cernysova O., Diversity of Saccharomyces cerevisiae killer strains in Lithuania, Botanica Lituanica, 2009, 15, 209–215 Bab’eva I.P., Golubev V.I., Methods of yeast isolation and identification, Moscow, Pischevaya promyshlennost, 1979 (in Russian) Leaw S.N., Chang H.C., Sun H.F., Barton R., Bouchara J.P., Chang T.C., Identification of medically important yeast species by sequence analysis of the internal transcribed spacer regions, J. Clin. Microbiol., 2006, 44, 693–699 Wang J., Analytical Electrochemistry, 2nd Edition, Wiley-WCH, 2000 Castro F.A.V., Herdeiro R.S., Panek A.D., Eleutherio E.C.A., Pereira M.D., Menadione stress in Saccharomyces cerevisiae strains deficient in the glutathione transferases, Biochim. Biophys. Acta, 2008, 1770, 213–220 Kim I.S., Sohn H.Y., Jin I., Adaptive stress response to menadione-induced oxidative stress in Saccharomyces cerevisiae KNU5377, J. Microbiol., 2011, 49, 816–823 Kraakman L., Lemaire K., Ma P., Teunissen A.W.R.H., Donaton M.C.V., van Dijck P., et al., A Saccharomyces cerevisiae G-protein coupled receptor, Gpr1, is specifically required for glucose activation of the cAMP pathway during the transition to growth on glucose, Mol. Microbial., 1999, 32, 1002–1012 Rolland F., de Winde J.H., Lemaire K., Boles E., Thevelein J.M., Winderkx J., Glucose-induced cAMP signaling in yeast requires both a G-protein coupled receptor system for extracellular glucose detection and a separable hexose kinase-dependent sensing process, Mol. Microbiol., 2000, 38, 348–358 Portela P., Moreno S., Glucose-dependent activation of protein kinase A activity in Saccharomyces cerevisiae and phosphorylation of its TPK1 catalytic subunit, Cell Signal., 2006, 18, 1072–1086 Kresnowati M.T.A. P., van Winden W.A., Almering M.J.H., ten Pierick A., Ras C., Knijnenburg T.A, et al., When transcriptome meets metabolome: fast cellular responses to sudden relief of glucose limitation, Mol. Syst. Biol., 2006, 2, 49 Sipiczki M., Metschnikovia strains isolated from botrytized grapes antagonize fungal and bacterial growth by iron depletion, Appl. Environ. Microbiol., 2006, 72, 6716–6724 Bendova O., The killer phenomena in yeasts, Folia Microbiol., 1986, 31, 422–433 Magliani, W. Conti S., Gerloni M., Bertolotti D., Polonelli L., Yeast killer systems, Clin. Microbiol. Rev., 1997, 10, 369–400 Marquina D., Santos A., Peinado J.M., Biology of killer yeasts, Int. Microbiol., 2002, 5, 65–71 Santos A., Mauro M.S., Bravo E., Marquina D., PMKT2, a new killer toxin from Pichia membranifaciens, and its promising biotechnological properties for control of the spoilage yeast Bretanomyces bruxellensis, Microbiol., 2009, 155, 624–634