Mechanistic modeling of the bioconcentration of (super)hydrophobic compounds in Hyalella azteca

Springer Science and Business Media LLC
Andrea Ebert1, Juliane Ackermann2, Kai‐Uwe Goss3
1Analytical Environmental Chemistry, Helmholtz Centre for Environmental Research—UFZ, 04318, Leipzig, Germany
2Section IV 2.3 “Chemicals”, Umweltbundesamt, 06844, Dessau-Roßlau, Germany
3Institute of Chemistry, Martin Luther University Halle-Wittenberg, 06120, Halle, Germany

Tóm tắt

AbstractBioconcentration tests using the freshwater amphipod Hyalella azteca as an alternative to conventional fish tests have recently received much attention. An appropriate computational model of H. azteca could help in understanding the mechanisms behind bioconcentration, in comparison to the fish as test organism. We here present the first mechanistic model for H. azteca that considers the single diffusive processes in the gills and gut. The model matches with the experimental data from the literature quite well when appropriate physiological information is used. The implementation of facilitated transport was essential for modeling. Application of the model for superhydrophobic compounds revealed binding to organic matter and the resulting decrease in bioavailable fraction as the main reason for the observed counterintuitive decrease in uptake rate constants with increasing octanol/water partition coefficient. Furthermore, estimations of the time needed to reach steady state indicated that durations of more than a month could be needed for compounds with a log Kow > 8, limiting the experimental applicability of the test. In those cases, model-based bioconcentration predictions could be a preferable approach, which could be combined with in vitro biotransformation measurements. However, our sensitivity analysis showed that the uncertainty in determining the octanol/water partition coefficients is a strong source of error for superhydrophobic compounds.

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Tài liệu tham khảo

Arnot JA, Gobas FAPC (2004) A food web bioaccumulation model for organic chemicals in aquatic ecosystems. Environ Toxicol Chem 23:2343–2355. https://doi.org/10.1897/03-438

Barker Jørgensen C, Møhlenberg F, Sten-Knudsen O (1986) Nature of relation between ventilation and oxygen consumption in filter feeders. Mar Ecol Prog Ser 29:73–88. https://doi.org/10.3354/meps029073

Bittermann K, Goss KU (2017) Predicting apparent passive permeability of Caco-2 and MDCK cell-monolayers: a mechanistic model. PLoS ONE 12:1–20. https://doi.org/10.1371/journal.pone.0190319

Böhm L, Schlechtriem C, Düring RA (2016) Sorption of highly hydrophobic organic chemicals to organic matter relevant for fish bioconcentration studies. Environ Sci Technol 50:8316–8323. https://doi.org/10.1021/acs.est.6b01778

Brown TN, Arnot JA, Wania F (2012) Iterative fragment selection: a group contribution approach to predicting fish biotransformation half-lives. Environ Sci Technol 46:8253–8260. https://doi.org/10.1021/es301182a

Burkhard LP (2000) Estimating dissolved organic carbon partition coefficients for nonionic organic chemicals. Environ Sci Technol 34:4663–4668. https://doi.org/10.1021/es001269l

Cantu MA, Gobas FAPC (2021) Bioaccumulation of dodecamethylcyclohexasiloxane (D6) in fish. Chemosphere 281. https://doi.org/10.1016/j.chemosphere.2021.130948

Chen CC, Kuo DTF (2018) Bioconcentration model for non-ionic, polar, and ionizable organic compounds in amphipod. Environ Toxicol Chem 37:1378–1386. https://doi.org/10.1002/etc.4081

Christie AE, Cieslak MC, Roncalli V et al (2018) Prediction of a peptidome for the ecotoxicological model Hyalellaazteca (Crustacea; Amphipoda) using a de novo assembled transcriptome. Mar Genomics 38:67–88. https://doi.org/10.1016/j.margen.2017.12.003

de Wolf W, Comber M, Douben P et al (2007) Animal use replacement, reduction, and refinement: development of an integrated testing strategy for bioconcentration of chemicals in fish. Integr Environ Assess Manag 3:3–17. https://doi.org/10.1897/1551-3793(2007)3[3:AURRAR]2.0.CO;2

EAS-E Suite (2022) (Ver.0.95 - BETA; release Feb.; 2022). Developed by ARC Arnot Research and Consulting Inc., Toronto, ON, Canada. www.eas-e-suite.com. Last accessed 24 Jan 2023

Erickson RJ, McKim JM (1990) A model for exchange of organic chemicals at fish gills: flow and diffusion limitations. Aquat Toxicol 18:175–197. https://doi.org/10.1016/0166-445X(90)90001-6

Everitt S, MacPherson S, Brinkmann M et al (2020) Effects of weathered sediment-bound dilbit on freshwater amphipods (Hyalellaazteca). Aquat Toxicol 228:105630. https://doi.org/10.1016/j.aquatox.2020.105630

Fredrick WS, Ravichandran S (2012) Hemolymph proteins in marine crustaceans. Asian Pac J Trop Biomed 2:496–502. https://doi.org/10.1016/S2221-1691(12)60084-7

Johanif N, Huff Hartz KE, Figueroa AE et al (2021) Bioaccumulation potential of chlorpyrifos in resistant Hyalellaazteca: implications for evolutionary toxicology. Environ Pollut 289:117900. https://doi.org/10.1016/j.envpol.2021.117900

Johnke R (1973) The influence of season upon the oxygen consumption of two populations of the freshwater 460 amphipod Hyalella aztecahyalella azteca. Master thesis (ocm60457213), Calif State Univ Fresno. http://hdl.handle.net/20.500.12680

Kelly BC, Gobas FAPC, McLachlan MS (2004) Intestinal absorption and biomagnification of organic contaminants in fish, wildlife, and humans. Environ Toxicol Chem 23:2324–2336. https://doi.org/10.1897/03-545

Kosfeld V, Fu Q, Ebersbach I et al (2020) Comparison of alternative methods for bioaccumulation assessment: scope and limitations of in vitro depletion assays with rainbow trout and bioconcentration tests in the freshwater amphipod Hyalellaazteca. Environ Toxicol Chem 39:1813–1825. https://doi.org/10.1002/etc.4791

Landrum PF, Scavia D (1983) Influence of sediment on anthracene uptake, depuration, and biotransformation by the amphipod Hyalellaazteca. Can J Fish Aquat Sci 40:298–305. https://doi.org/10.1139/f83-044

Landrum PF, Steevens JA, Gossiaux DC et al (2004) Time-dependent lethal body residues for the toxicity of pentachlorobenzene to Hyalellaazteca. Environ Toxicol Chem 23:1335–1343. https://doi.org/10.1897/03-164

Landrum PF, Steevens JA, McElroy M et al (2005) Time-dependent toxicity of dichlorodiphenyldichloroethylene to Hyalellaazteca. Environ Toxicol Chem 24:211–218. https://doi.org/10.1897/04-055R.1

Larisch W, Brown TN, Goss KU (2017) A toxicokinetic model for fish including multiphase sorption features. Environ Toxicol Chem 36:1538–1546. https://doi.org/10.1002/etc.3677

Larisch W, Goss KU (2018) Modelling oral up-take of hydrophobic and super-hydrophobic chemicals in fish. Environ Sci Process Impacts 20:98–104. https://doi.org/10.1039/c7em00495h

Lee JH, Landrum PF, Koh CH (2002) Toxicokinetics and time-dependent PAH toxicity in the amphipod Hyalellaazteca. Environ Sci Technol 36:3124–3130. https://doi.org/10.1021/es011201l

Lotufo GR, Landrum PF, Gedeon ML et al (2000) Comparative toxicity and toxicokinetics of DDT and its major metabolites in freshwater amphipods. Environ Toxicol Chem 19:368–379. https://doi.org/10.1002/etc.5620190217

Nuutinen S, Landrum PF, Schuler LJ et al (2003) Toxicokinetics of organic contaminants in Hyalellaazteca. Arch Environ Contam Toxicol 44:467–475. https://doi.org/10.1007/s00244-002-2127-x

OECD (2012) Test No. 305: Bioaccumulation in fish: aqueous and dietary exposure. https://www.oecd-ilibrary.org/environment/test-no-305-bioaccumulation-in-fish-aqueous-and-dietary-exposure_9789264185296-e. Accessed 30 Mar 2022

Othman MS, Pascoe D (2001) Growth, development and reproduction of Hyalellaazteca (Saussure, 1858) in laboratory culture. Crustaceana 74:171–181. https://doi.org/10.1163/156854001750096274

Regan S, Hynds P, Flynn R (2017) An overview of dissolved organic carbon in groundwater and implications for drinking water safety. Hydrogeol J 25:959–967. https://doi.org/10.1007/s10040-017-1583-3

Schlechtriem C, Kampe S, Bruckert HJ et al (2019) Bioconcentration studies with the freshwater amphipod Hyalellaazteca: are the results predictive of bioconcentration in fish? Environ Sci Pollut Res 26:1628–1641. https://doi.org/10.1007/s11356-018-3677-4

Schlechtriem C, Kosfeld V, Pandard P, Rauert C (2021) Validation of the hyalella azteca bioconcentration test (HYBIT). Abstract 4.03.13 from Setac Europe 31st Annual Meeting, Sevilla, Spain

Schlechtriem C, Kühr S, Müller C (2022) Development of a bioaccumulation test using Hyalella azteca, Forschungskennzahl 3718 67 401 0, UBA-FB000548/ENG. Umweltbundesamt

Schuler LJ, Landrum PF, Lydy MJ (2004) Time-dependent toxicity of fluoranthene to freshwater invertebrates and the role of biotransformation on lethal body residues. Environ Sci Technol 38:6247–6255. https://doi.org/10.1021/es049844z

Trowell JJ, Gobas FAPC, Moore MM, Kennedy CJ (2018) Estimating the bioconcentration factors of hydrophobic organic compounds from biotransformation rates using rainbow trout hepatocytes. Arch Environ Contam Toxicol 75:295–305. https://doi.org/10.1007/s00244-018-0508-z

Westergaard H, Dietschy JM (1976) The mechanism whereby bile acid micelles increase the rate of fatty acid and cholesterol uptake into the intestinal mucosal cell. J Clin Invest 58:97–108. https://doi.org/10.1172/JCI108465

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