Matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) of membrane proteins and non‐covalent complexes

Journal of Mass Spectrometry - Tập 30 Số 10 - Trang 1462-1468 - 1995
Burkhard Rosinke1, Kerstin Strupat2, Franz Hillenkamp2, Jürg P. Rosenbusch3, Norbert A. Dencher4, Ulrike Krüger1, Hans‐Joachim Galla1
1Institute of Biochemistry, University of Münster, Wilhelm-Klemm-Str. 2, D-48149 Münster, Germany
2Institute for Medical Physics and Biophysics, University of Münster, Robert-Koch-Str. 31, D-48149 Münster, Germany
3Maurice-Müller Institute, Biozentrum Basel, Klingelbergstr. 70, CH4057 Basel, Switzerland
4Institute of Biochemistry, Technical University of Darmstadt, Petersenstr. 22, D-64287 Darmstadt, Germany

Tóm tắt

AbstractMatrix‐assisted laser desorption/ionization (MALDI) mass spectra and methods to improve their quality are reported for three hydrophobic, membrane‐bound proteins: porin from Escherichia coli, bacteriorhodopsin from Halobacterium salinarium and cholesterolesterase from Pseudomonas fluorescens. Several commonly used UV and IR matrices have been tested. In addition, the susceptibility of MALDI mass spectrometry to various neutral and ionic detergents, known usually to degrade the quality of MALDI mass spectra, has been tested systematically. For porin, consisting of three identical non‐covalently bound subunits, a new sample preparation is reported, resulting in the desorption of the intact quaternary protein structure. This leads to a better understanding of the way a given analyte is embedded into the host matrix crystals.

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Thông tin xuất bản

Journal of Mass Spectrometry

Tập 30 Số 10

1462-1468

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