Maternal nutrient supplementation counteracts bisphenol A-induced DNA hypomethylation in early development

Proceedings of the National Academy of Sciences of the United States of America - Tập 104 Số 32 - Trang 13056-13061 - 2007
Dana C. Dolinoy1,2,3, Dale Huang4,5, Randy L. Jirtle4,2,3
1*Department of Radiation Oncology and University Program in Genetics and Genomics, Duke University, Durham, NC 27710, USA.
2Integrated Toxicology and Environmental Health Program, Duke University, Durham, NC 27708
3University Program in Genetics and Genomics, Duke University, Durham, NC 27710; and
4Department of Radiation Oncology; and
5Duke University

Tóm tắt

The hypothesis of fetal origins of adult disease posits that early developmental exposures involve epigenetic modifications, such as DNA methylation, that influence adult disease susceptibility. In utero or neonatal exposure to bisphenol A (BPA), a high-production-volume chemical used in the manufacture of polycarbonate plastic, is associated with higher body weight, increased breast and prostate cancer, and altered reproductive function. This study shows that maternal exposure to this endocrine-active compound shifted the coat color distribution of viable yellow agouti ( A vy ) mouse offspring toward yellow by decreasing CpG (cytosine-guanine dinucleotide) methylation in an intracisternal A particle retrotransposon upstream of the Agouti gene. CpG methylation also was decreased at another metastable locus, the CDK5 activator-binding protein ( Cabp IAP ). DNA methylation at the A vy locus was similar in tissues from the three germ layers, providing evidence that epigenetic patterning during early stem cell development is sensitive to BPA exposure. Moreover, maternal dietary supplementation, with either methyl donors like folic acid or the phytoestrogen genistein, negated the DNA hypomethylating effect of BPA. Thus, we present compelling evidence that early developmental exposure to BPA can change offspring phenotype by stably altering the epigenome, an effect that can be counteracted by maternal dietary supplements.

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Thông tin xuất bản

Proceedings of the National Academy of Sciences of the United States of America

Tập 104 Số 32

13056-13061

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