Mapping Modifiers Affecting Muscularity of the Myostatin Mutant (<i>MstnCmpt-dl1Abc</i>) Compact Mouse

Genetics - Tập 165 Số 1 - Trang 257-267 - 2003
L. Varga1, Géza Müller2, Gyula Szabó1, O. Pinke1, Edit Korom1, Balázs Kovács1, László Patthy3, M. Soller4
1Institute for Animal Biology, Agricultural Biotechnology Center, H-2101 Gödöllö, Hungary
2EGIS Pharmaceuticals, H-1475 Budapest, Hungary
3Institute of Enzymology, Hungarian Academy of Sciences, H-1518 Budapest, Hungary
4Department of Genetics, The Alexander Silberman Life Science Institute, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel

Tóm tắt

AbstractThe hypermuscular Compact phenotype was first noted in a line of mice selected for high body weight and protein content. A new line, based on mice showing the Compact phenotype, was formed and selected for maximum expression of the Compact phenotype. Previously we mapped and identified a 12-bp deletion in the myostatin gene, denoted MstnCmpt-dl1Abc, which can be considered as a major gene responsible for the hypermuscular phenotype. Genetic analysis revealed that full expression of the hypermuscular phenotype requires the action of modifier loci in addition to MstnCmpt-dl1Abc. To map these modifier loci, an interspecific F2 population was generated between Comp9, an inbred line homozygous for MstnCmpt-dl1Abc, and CAST/Ei, an inbred line generated from Mus musculus castaneus. Selective DNA pooling and genotyping, separately by gender, was carried out within a subpopulation of the F2 consisting of individuals homozygous for MstnCmpt-dl1Abc. Significant association with hypermuscularity at a false discovery rate (FDR) of 0.05 was found for markers on chromosomes 3, 5, 7, 11, 16, and X. In all cases, the marker allele derived from the Comp9 parent showed a higher frequency in the hypermuscular group and the CAST/Ei allele in the normal group. The modifier loci apparently exerted their effects on muscularity only in the presence of MstnCmpt-dl1Abc.

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Genetics

Tập 165 Số 1

257-267

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